Latar Belakang : Budaya Keselamatan pasien merupakan fondasi keselamatan pasien. Membangun budaya keselamatan pasien merupakan kata kunci terwujudnya pelayanan yang bermutu dan aman, terdapat beberapa faktor yang mempengaruhi penerapan budaya keselamatan pasien yaitu salah satunya yaitu kerjasama tim. Tujuan Penelitian : Untuk mengetahui hubungan kerjasama tim dengan penerapan budaya keselamatan pasien di ruang rawat inap Rumah Sakit Bhayangkara Palembang Tahun 2018. Metode Penelitian : Jenis penelitian ini adalah quantitative descriptive study dengan pendekatan cross sectional. Teknik pengambilan sampel yaitu total sampling sebanyak 42 responden. Alat pengumpulan data berupa kuesioner tentang kerjasama tim, dan penerapan budaya keselamatan pasien. Hasil : Kerjasama tim baik 30 responden (71,4%), dan penerapan budaya keselamatan pasien baik 28 responden (66,7%). Hasil uji statistik menunjukkan bahwa ada hubungan kerjasama tim dengan penerapan budaya keselamatan pasien (p value 0,009). Kesimpulan: Perlu diadakan evaluasi standar kriteria seorang leader tim dengan merumuskan niai-nilai professional perawat, perlu adanya seorang leader yang yang dapat mendrong anggota tim dalam membudayakan komunikasi yang terbuka, adanya evaluasi tim baik individu maupun kelompok melalui kegiatan informal ruang rawat, bangun kesadaran akan nilai keselamatan pasien dengan menerapkan budaya keselamatan pasien di semua lini tatanan rumah sakit mulai dari perawat pelaksana sampai ke level manajer di semua ruang rawat inap Rumah Sakit Bhayangkara Palembang. Background: The culture of patient safety is the foundation of patient safety. Building a patient safety culture is the key word for creating good and safe service, there are several factors that influence the implementation of patient safety culture, one of them is team work. Research Objectives: To find out the correlation between teamwork and the implementation of patient safety culture hospitalization room of Bhayangkara Hospital Palembang 2018. Research Method: This study was quantitative descriptive study with cross sectional approach. The total sample that has been collected through total sampling were 42 respondents. The questionnaire used in this study consisted of 19 questions of team work, and 30 questions of patient safety culture implementation. Results: Good teamwork consisted of 30 respondents (71.4%), and patient safety culture implementation consisted 28 respondents (66.7%). The result of statistical test showed that there was a correlation between teamwork and patient safety culture implementation (ρ value 0,009). Conclusions and Suggestion: It is necessary to evaluate the criteria standards of a team leader by formulating the values of professional nurses, the need for a leader who can support team members in cultivating open communication, evaluating both individual and group teams through informal activities in the ward, building awareness of the value of safety patients by applying a culture of patient safety in all lines of the hospital setting from the nurse level to the manager level at all inpatient rooms of Bhayangkara Hospital Palembang.