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All Journal ACTA VETERINARIA INDONESIANA Hemera Zoa ARSHI Veterinary Letters Jurnal Kedokteran Hewan
Aprilia Hardiati
Divisi Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Phenotypic and Genotypic Study of Antibiotics Resistance Profile in Escherichia coli Isolated from Broilers in Cianjur, Indonesia Aprilia Hardiati; Safika; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu
Acta VETERINARIA Indonesiana Vol. 9 No. 2 (2021): Juli 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.2.97-104

Abstract

This study aimed to investigate the phenotypic and genotypic of antibiotics resistance profile in Escherichia coli. The 30 samples come from cloacal swab of broilers in Cianjur, Indonesia. Isolation and identification of E. coli was performed by culture in McConkey agar, eosin methylene blue agar, Gram staining and five essential biochemical tests (IMViC). In this study, 10 isolates (33.3%) were confirmed E. coli positive. Phenotypic profile was performed by screening all isolates with 8 antibiotics of 6 antibiotic groups. The screening was carried by Kirby-Bauer disk diffusion method based on the standard of CLSI. For genotypic profile, each resistant isolate was detected antibiotic resistance-encoding gene. The result showed all isolates (100%) resistant against tetracyclin, oxytetracycline and erythromycin. Nine isolates (90%) detected nalidixic acid and enrofloxacin-resistant. The ciprofloxacin and gentamicin-resistant isolates were 70% and 40%, respectively. There was no resistant isolate for chloramphenicol. Multi drug-resistant was detected on 90% isolates. Only gyrA (100%) and tetA (80%) genes were detected. This study showed high rate of occurrence of antibiotic resistance in E. coli. Not all resistant isolates were detected in the antibiotic resistance-encoding gene in this study. Future research to detect resistance genes should use more varied target genes.
MP-14 Antibiotics Resistance Patterns of Escherichia coli Isolated from Poultry in West Java Aprilia Hardiati; . Safika; Fachriyan Hasmi Pasaribu
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.213 KB)

Abstract

Most of poultry industries use antibiotics for health management program. They use them as bacterial infection treatment and disease prevention, known as antibiotic growth promotor (AGP) [1]. Now, worldwide concern is about antibiotics resistance. Monitoring programs are done by countries in the world to protect human and animal health [2]. The monitoring programs usually use indicator bacteria such as Escherichia coli [3].Many researchers studied about antibiotics resistance in Indonesia. Escherichia coli isolated from poultry has been resistance to doxycycline (25%) and gentamycin (12.5%) [4]. Seven E. coli isolated from fecal samples shown that resistance to methicillin (85.7%), penicillin G (71.4%) and 42,9% were resistance both doxycycline hydrochloride and streptomycin [5]. Start from Januari 1st 2018 Indonesian Misintry of Agricuture banned antibiotics as AGP, based on Permentan No. 14/2017. They tried to prevent the spread of antibiotics resistance. So, it is necessary to determine antibiotics resistance patterns, especially in E. coli as indicator bacteria.
Salmonella sp. isolat ayam pedaging dari Cianjur: isolasi, identifikasi, dan resistansi antibiotika Aprilia Hardiati; Nanda Fadli Kurnia
ARSHI Veterinary Letters Vol. 5 No. 3 (2021): ARSHI Veterinary Letters - Agustus 2021
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.3.57-58

Abstract

Salmonella sp. merupakan salah satu bakteri penyebab penyakit serius pada ayam yang telah banyak mengalami resistansi antibiotik. Penelitian ini bertujuan untuk melakukan isolasi dan identifikasi Salmonella sp. dari ayam pedaging di Cianjur serta mengetahui resistansinya terhadap tujuh antibiotik. Tiga puluh swab kloaka ayam pedaging 9 diantaranya terisolasi dan teridentikasi Salmonella sp. kemudian diuji terhadap antibiotik ampisilin-sulbaktam, tetrasiklin, oksitetrasiklin, doksisiklin, eritromisin, asam nalidiksat, dan siprofloksasin menggunakan metode difusi cakram menurut Kirby-Bauer. Interpretasi hasil disesuaikan dengan Clinical and Laboratory Standards Institute 2018. Salmonella sp. berhasil diisolasi dan diidentifkasi dari swab kloaka ayam pedaging di Cianjur. Tetrasiklin, oksitetrasiklin, doksisiklin, eritromisin, dan asam nalidiksat, tidak efektif untuk menghamnbat pertumbuhan Salmonella sp. dari ayam pedaging di Cianjur.
MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA Aprilia Hardiati; Safika Safika; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.056 KB) | DOI: 10.21157/j.ked.hewan.v16i1.18944

Abstract

This study was aimed at isolating and identifying Salmonella sp. and then conducting an antibiotics susceptibility test in order to detect resistant genes.  One hundred and five chicken cloaca swab samples were used in this study. 30 samples were taken from a layer farm in Bogor, 45 from a broiler farm in Sukabumi and 30 from a broiler farm in Cianjur. In order to  isolate and identify the bacteria, a tetrathionate broth was used, which was then cultured in a Salmonella-Shigella agar, and finally a Gram stain and biochemical test was conducted. To confirm the presence of Salmonella sp., a pair of primers were used for the polymerase chain reaction (PCR) method to determine the presence of the invA gene.. An antibiotics susceptibility test was used with the Kirby-Bauer disk diffusion method. Nine antibiotics were used in this study. Each primer pair was used for the detection of tetA, blaTEM, aac(3)-IV, gyrA and ermB genes, and for genes encoding antibiotic resistance  a PCR test was used. Eight (7.6%) Salmonella sp. were  isolated in this study. All isolates showed positive results with PCR confirmation. The results of the antibiotics susceptibility test showed that Salmonella sp. isolates were resistant to tetracycline (75%), oxytetracycline (75%), amphicillin (75%), gentamycin (12.5%), nalidixic acid (100%), ciprofloxacin (12.5%), enrofloxacin (0%), erythromycin (100%), and chloramphenicol (0%). The distribution of antibiotic resistance genes in Salmonella sp. were tetA (33.3%), blaTEM (100%), aac(3)-IV (0%), gyrA (100%) and ermB (0%) positive. In conclusion, Salmonella sp. was isolated. All isolates showed positive results in the PCR confirmation. Salmonella sp. isolates were resistant to tetracycline, oxytetracycline, amphicillin, gentamycin, nalidixic acid, ciprofloxacin, and erythromycin. Only the tetA, blaTEM, and gyrA genes were detected in Salmonella sp. isolates.
MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA Aprilia Hardiati; Safika Safika; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.18944

Abstract

This study was aimed at isolating and identifying Salmonella sp. and then conducting an antibiotics susceptibility test in order to detect resistant genes.  One hundred and five chicken cloaca swab samples were used in this study. 30 samples were taken from a layer farm in Bogor, 45 from a broiler farm in Sukabumi and 30 from a broiler farm in Cianjur. In order to  isolate and identify the bacteria, a tetrathionate broth was used, which was then cultured in a Salmonella-Shigella agar, and finally a Gram stain and biochemical test was conducted. To confirm the presence of Salmonella sp., a pair of primers were used for the polymerase chain reaction (PCR) method to determine the presence of the invA gene.. An antibiotics susceptibility test was used with the Kirby-Bauer disk diffusion method. Nine antibiotics were used in this study. Each primer pair was used for the detection of tetA, blaTEM, aac(3)-IV, gyrA and ermB genes, and for genes encoding antibiotic resistance  a PCR test was used. Eight (7.6%) Salmonella sp. were  isolated in this study. All isolates showed positive results with PCR confirmation. The results of the antibiotics susceptibility test showed that Salmonella sp. isolates were resistant to tetracycline (75%), oxytetracycline (75%), amphicillin (75%), gentamycin (12.5%), nalidixic acid (100%), ciprofloxacin (12.5%), enrofloxacin (0%), erythromycin (100%), and chloramphenicol (0%). The distribution of antibiotic resistance genes in Salmonella sp. were tetA (33.3%), blaTEM (100%), aac(3)-IV (0%), gyrA (100%) and ermB (0%) positive. In conclusion, Salmonella sp. was isolated. All isolates showed positive results in the PCR confirmation. Salmonella sp. isolates were resistant to tetracycline, oxytetracycline, amphicillin, gentamycin, nalidixic acid, ciprofloxacin, and erythromycin. Only the tetA, blaTEM, and gyrA genes were detected in Salmonella sp. isolates.
Co-Authors Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu I wayan Teguh Wibawan Nanda Fadli Kurnia Safika S, Safika