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All Journal Pharmaciana: Jurnal Kefarmasian Prosiding Indonesian Journal of Chemistry CARADDE: Jurnal Pengabdian Kepada Masyarakat Molekul: Jurnal Ilmiah Kimia Jurnal Rekayasa Kimia & Lingkungan Fokus Bisnis: Media Pengkajian Manajemen dan Akuntansi

Undri Rastuti

Jurusan Kimia, Fakultas MIPA Universitas Jenderal Soedirman

Author-ID : 3035034

Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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KARAKTERISASI ENZIM AMILASE DARI BAKTERI Bacillus amyloliquefaciens Ningsih, Dian Riana; Rastuti, Undri; Kamaludin, Ridlwan
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Enzim Amilase merupakan salah satu jenis enzim yang berperan penting dalam industri. Enzim amilase digunakan untuk menghidrolisis pati menjadi molekul karbohidrat yang lebih sederhana, yaitu dekstrin, maltosa dan glukosa. Industri yang menggunakan amilase antara lain: dalam industri kertas, industry detergen, industry tekstil, industry obat dan industri roti dan kue. Bacillus amyloliquefacien merupakan salah satu bakteri yang dapat menghasilkan amilase. Tujuan dari penelitian ini adalah menentukan aktivitas enzim amylase dan mengkarakterisasi sifat biokimia enzim amylase dari B. amyloliquefaciens. Tahapan penelitian ini adalah penentuan waktu produksi optimum enzim amylase, produksi amylase dan penentuan aktivitas enzim amylase pada berbagai suhu dan pH. Penentuan aktivitas amylase menggunakan metode Nelson Somogyi. Hasil penelitian menunjukkan bahwa enzim amylase yang dihasilkan oleh B. amyloliquefaciens mempunyai waktu produksi optimum pada jam ke 24 (1.4986 U/ml), temperature optimum 30-60 oC dan pH optimum 6-7.

SKRINING SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETILASETAT DAUN WEDUSAN (Eupatorium odoratum) Purwati Purwati; Undri Rastuti
Molekul Vol 4, No 2 (2009)
Publisher : Universitas Jenderal Soedirman

Oxidation process is a natural process which always occurs in fat. The process affects and decreases the fat quality. Oxidation in fat can be hampered by the addition of antioxidant. Antioxidant activity of wedusan leaf has to be studied to know the possibility of wedusan leaf as an antioxidant. Hence, the aims of the research were to determine the antioxidant activity of ethyl acetate extract of wedusan leaf using TBA method, and to compare the antioxidant activity of wedusan leaf and that of BHT. The research method consisted of sample preparation, extraction, and determination of antioxidant activity using TBA method. Wedusan leaf was extracted by maceration using n-hexane and ethyl acetate solvents. The n-hexane extract was 2.90 gram, whereas ethyl acetate extract was 13.12 gram. Based on qualitative screening on secondary metabolites, ethyl acetate extract contained flavonoid. The results from GC-MS indicated that ethyl acetate extract contained methyl heptadecanoic, methyl-13-octadecenoic, 14,16-octadecadienal, and octadecanoic acid. The order of inhibition activity of antioxidant were 0.05% (w/v) of BHT > 0.15% (w/v) of ethyl acetate extract > 0.10% (w/v) of ethyl acetate extract > 0.05% (w/v) of ethyl acetate extract.

AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN PALA DARI BANYUMAS TERHADAP Staphylococcus aureus DAN Escherichia coli SERTA IDENTIFIKASI SENYAWA PENYUSUNNYA Undri Rastuti; Senny Widyaningsih; Dwi Kartika; Dian Riana Ningsih
Molekul Vol 8, No 2 (2013)
Publisher : Universitas Jenderal Soedirman

Staphylococcus aureus dan Escherichia coli adalah bakteri yang dapat menyebabkan infeksi. Umumnya masyarakat dalam mengobati penyakit infeksi terhadap bakteri sering menggunakan antibiotik, namun apabila digunakan secara berlebihan dan kurang terarah dapat mengakibatkan terjadinya resistensi. Untuk mengatasinya diperlukan pencarian bahan alami sebagai alternatif pengobatan, salah satunya yaitu minyak atsiri daun pala. Penelitian inibertujuan untuk mengetahui aktivitas antibakteri minyak atsiri daun pala dari Banyumas terhadap S. aereus dan E. coli serta mengidentifikasi senyawa penyusunnya. Minyak atsiri dari serbuk daun pala kering diisolasi menggunakan metode destilasi air. Minyak atsiri yang diperoleh diuji sifat fisik dan dilakukan identifikasi senyawa penyusunnya menggunakan GC-MS. Pengujian aktivitas antibakteri dilakukan dengan menggunakan metode difusi untuk mengetahui Konsentrasi Hambat Tumbuh Minimum (KHTM) terhadap S. aereus dan E. coli. Rendemen minyak atsiri daun pala yang diperoleh sebesar 1,34%. Minyak atsiri ini berwarna kuning pucat, berbau khas minyak pala dengan indeks bias sebesar 1,4779 dan bobot jenis sebesar 0,8862 g/cm3. Minyak atsiri daun pala diketahui memiliki 33 komponen kimia dan 5 komponen kimia terbesarnya adalah sabinene, terpinene-4-ol, α-pinene, β-pinene, and β-phellandrene. Minyak atsiri daun pala terbukti memiliki aktivitas antibakteri terhadap S. aureus dengan KHTM pada konsentrasi minyak atsiri 3,125% menghasilkan zona hambat sebesar 16,81 mm dan terhadap E. coli dengan KHTM pada konsentrasi minyak atsiri 1% menghasilkan zona hambat sebesar 0,54 mm.

UJI AKTIVITAS ANTIOKSIDAN HASIL DEGRADASI LIGNIN DARI SERBUK GERGAJI KAYU KALBA (Albizia falcataria) DENGAN METODE TBA (Thio Barbituric Acid) Undri Rastuti; Purwati Purwati
Molekul Vol 5, No 2 (2010)
Publisher : Universitas Jenderal Soedirman

Antioxidants are compounds that can delay, retard or inhibit the oxidation reaction. Lignin is a natural polymer consisting of monomeric substituted phenols. Wood lignin degradation Kalba (Albizia falcataria) yields substituted phenol. The purpose of this study was to test the antioxidant activity of compounds of lignin degradation products Kalba using TBA (Thiobarbituric Acid). Wood lignin degradation products Kalba tested antioxidant activity using the TBA method. Phase test phase of this antioxidant activity is sample preparation, determination of the maximum wavelength, determination of equilibrium time, absorbance measurements and determination of the percentage of inhibition. The wavelength maximum for BHT test solution was obtained at 530 nm. The stability of absorbance achieved after 80 minutes equilibrium time. BHT test solution and sample solution containing the degradation of lignin 0.10% (w/v) increased but not as sharp as the absorbance of control, this suggests that the degradation of wood lignin Kalba have activity as an antioxidant, which relative minimize 13,70 % compare with BHT.

PENERAPAN METODE ELEKTROKIMIA UNTUK PENURUNAN CHEMICAL OXYGEN DEMAND (COD) DAN TOTAL SUSPENDED SOLID (TSS) LIMBAH CAIR INDUSTRI TAHU Suyata Suyata; Irmanto Irmanto; Undri Rastuti
Molekul Vol 10, No 1 (2015)
Publisher : Universitas Jenderal Soedirman

Limbah cair industri tahu memiliki nilai COD dan TSS yang tinggi, apabila dibuang ke badan perairan akan menyebabkan pencemaran lingkungan. Oleh karena itu, perlu dilakukan penurunan nilai COD dan TSS limbah cair industri tahu tersebut sebelum dibuang ke badan perairan. Penurunan nilai COD dan TSS limbah cair industri tahu dapat dilakukan menggunakan metode elektrokimia. Penelitian ini bertujuan untuk mengetahui pengaruh voltase, jarak elektroda, pH, dan waktu elektrolisis terhadap penurunan nilai COD dan TSS limbah cair industri tahu. Eksperimen dilakukan dengan mengelektrolisis limbah cair industri tahu menggunakan anoda PbO2 dan katoda Pb. Hasil penelitian menunjukkan bahwa dibawah kondisi optimum pada voltase 12V, jarak elektroda 1 cm, pH 1 dan waktu elektrolisis selama 120 menit, penurunan nilai COD dan TSS mencapai 96,33% dan 87,87%

UJI AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN KALBA (Albizia falcataria) DENGAN METODE DPPH(1,1-Difenil-2-pikrilhidrazil) DAN IDENTIFIKASI SENYAWA METABOLIT SEKUNDERNYA Undri Rastuti* dan Purwati Undri Rastuti; Purwati Purwati
Molekul Vol 7, No 1 (2012)
Publisher : Universitas Jenderal Soedirman

Telah dilakukan penelitian uji aktivitas antioksidan ekstrak daun kalba pada pelarut n-heksana, etil asetat dan metanol serta mengidentifikasi golongan senyawa metabolit sekundernya. Hasil maserasi diperoleh ekstrak n-heksana (E1), etil asetat (E2) dan metanol (E3) daun kalba berbentuk pasta dengan rendemen masing-masing ekstrak adalah 2,49, 5,08 dan 5,32% (b/b). Uji aktivitas antioksidan E1, E2, E3 memiliki nilai ES50 masing-masing sebesar 1338,758, 473,756 dan 264,519 ppm. Ekstrak metanol daun kalba memilki aktivitas antioksidan paling tinggi. Uji senyawa metabolit sekunder pada ekstrak metanol daun kalba menunjukkan senyawa metabolit sekunder golongan terpenoid, flavonoid dan fenolat. Hasil analisis menggunakan spektrofotometer UV-Vis menunjukkan adanya gugus kromofor C=C dan hasil analisis menggunakan spektrofotometer IR menunjukkan bahwa ekstrak metanol daun kalba memiliki gugus fungsi O-H, C-H alifatik, C=C alkena dan C=C aromatic.

Perencanaan Peningkatan Kapasitas Produksi Tepung Mendoan Instan Undri Rastuti; Hartiwi Diastuti; Sutarmin Sutarmin; Widhiatmoko Herry Purnomo
CARADDE: Jurnal Pengabdian Kepada Masyarakat Vol. 2 No. 2 (2020): Februari
Publisher : Ilin Institute

Abstrak. Mendoan adalah makanan khas masyarakat Banyumas yang berbahan dasar tempe. Bagi masyarakat Banyumas makan mendoan itu sudah merupakan budaya setiap hari. Tiada hari tanpa makan mendoan. Untuk membuat mendoan yang mudah dan cepat diperlukan tepung yang praktis/instan/siap pakai. Produksi tepung mendoan instan dalam skala rumah tangga sangat terbatas dan selalu kewalahan melayani permintaan konsumen. Setiap hari hasil produksinya habis terjual, bahkan sampai menolak pesanan.. Untuk mengatasi permasalahan-permasalah tersebut tim pengabdian masyarakat Universitas Jenderal Soedirman dan Universitas Peradaban berusaha untuk membantu meningkatkan kapasitas produksi dengan cara memberikan bantuan alat-alat produksi. Selain itu juga akan dilakukan pendampingan, penyuluhan dan pelatihan meliputi: penggunaan dan perawatan alat-alat modern.

THE ACTIVE FRACTION FROM Nigella sativa AND ITS ACTIVITY AGAINST T47D CELL LINE Heny Ekowati; Eka Prasasti; Undri Rastuti
Indonesian Journal of Chemistry Vol 11, No 3 (2011)
Publisher : Universitas Gadjah Mada

Breast cancer is one of the main causes of death in women. Cancer treatment with surgery, chemotherapy, and radiology often cause undesirable side effects. Therefore, alternative cancer treatment by using plants as traditional medicine was expected to reduce side effects. Nigella sativa is one of the plants used as anticancer empirically. This study conducted to examine the cytotoxic activity of Nigella sativa seeds and identify its components on T47D breast cancer cells. Petroleum ether, chloroform, ethyl acetate, and ethanol were used to extract N. sativa seeds. The extracts were tested their cytotoxic activity on T47D cell line using MTT method. The active compound was separated using column chromatography. Cytotoxic test on T47D cell line was perform for extracts of each separation stage. Data were analyzed by probit analysis to obtain IC50 values. Components identification was performed using GC-MS analysis. The results showed that chloroform extract has cytotoxic activity better than other extracts with IC50 of 124.206 µg/mL. The third fraction has cytotoxic activity better than other fractions with IC50 of 68.568 µg/mL. The GC-MS analysis showed that in the third fraction of the chloroform extract contain linoleat acid, the major compound and tryptamine.

SYNTHESIS OF 6-NITRO VERATRYL ALCOHOL AND 6-NITRO VERATRALDOXIM FROM VANILIN AS INTERMEDIATES FOR THE PREPARATION OF C-9154 ANTIBIOTIC DERVATIVES Jumina Jumina; Undri Rastuti; Sabirin Matsjeh
Indonesian Journal of Chemistry Vol 3, No 1 (2003)
Publisher : Universitas Gadjah Mada

The synthesis of 6-nitro veratryl alcohol and 6-nitro veratraldoxim from vanilin which was required as intermediates for the preparation of C-9154 antibiotic derivatives was carried out. C-9154 antibiotic is a sufficiently potent antibiotic, but so far this is produced only in low yields through microbiological processes. The reaction steps performed were (1) methylation of vanilin, (2) nitration of the methylation product, (3) reduction of the corresponding nitro compound and (4) reaction of the nitration product with HO-NH2.HCl. Methylation of vanilin was conducted using dimethylsulfate and NaOH at 60 oC for 2 hours. Nitration of the methylation product was performed in 2 methods, i.e. using neat HNO3 and using a mixture of HNO3 and H2SO4 both at 5 oC for 2 hours. Reduction of the nitration product was conducted using NaBH4 either at room temperature and at reflux. Reaction of the nitration product with HO-NH2.HCl was carried out in ethanol 95% at 50 oC for 2 hours. The products were analyzed by means of TLC, GC, IR, 1H NMR and GC-MS spectrometers. The methylation of vanilin gave 87.7% yield of veratraldehyde which was found as a white crystal (m.p 43 oC). The nitration of veratraldehyde produced 6-nitro veratraldehyde observed as a yellow crystal having of m.p. 130 oC. Nitration using neat HNO3 gave a smaller yield (50.35%) of 6-nitro veratraldehyde than nitration with a mixture of HNO3 and H2SO4 (93.63%). Reduction of 6-nitro veratraldehyde using NaBH4 at room temperature and at reflux afforded 6-nitro veratryl alcohol which was found as brown crystal (m.p 123-127 oC) respectively in 13.47% and 56.61%. This reduction also produced 6-amino veratryl alcohol and 3,4-dimethoxy benzoic zcid as by products. 6-Nitro veratraldehyde reacts with HO-NH2.HCl to give 6-nitro veratraldoxim in 48.27% yield.

SINTESIS 6-NITRO VERATRALDEHID (3,4-DIMETOKSI-6-NITRO BENZALDEHID) DARI VANILIN DENGAN HNO3 DAN CAMPURAN HNO3-H2SO4 Undri Rastuti; Jumina Jumina; Sabirin Matsjeh
Molekul Vol 4, No 2 (2009)
Publisher : Universitas Jenderal Soedirman

The synthesis of 6-nitro veratraldehyde from vanillin was used HNO3 and a mixture of HNO3 and H2SO4. The reaction steps were (I) methylation of vanillin and (2) nitration of the methylation product. Methylation of vanillin was conducted using dimetnylsulfate and NaOH at 60 0C for 2 hours. Nitration of the methylation product was performed in two methods, which using HN03 and using a mixture of HN03 and H2SO4 both at 5 0C for 2 hours. The products were analyzed by means of TLC, GC; IR, 1H-NMR and GC-MS spectrometers.The methylation of vanillin gave 87.7 % yield of veratraldehyde which was found as a white crystal (m.p 43 oC). The nitration of veratraldehyde produced 6-nitro veratraldehyde observed as a yellow crystal having of m.p. 1300C. Nitration using neat HNO3 gave a smaller yield (50.35%) of 6-nitro veratraldehyde than nitration with a mixture of HNO3 and H2SO4 (93.63 %).

Co-Authors Dian Riana Ningsih Dian Riana Ningsih Dwi Kartika Eka Prasasti Nur Rachmani Hartiwi Diastuti Heny Ekowati Irmanto Irmanto Jumina Jumina Jumina Jumina Mochamad Chasani Purwati Purwati Purwati Purwati Ridlwan Kamaludin Ryan Aditya Mahendra Sabirin Matsjeh Sabirin Matsjeh Senny Widyaningsih Senny Widyaningsih Senny Widyaningsih Sity Khalidah Zia Sutarmin Sutarmin, Sutarmin Suyata Suyata Umi Salamah Uyi Sulaeman Uyi Sulaeman Widhiatmoko Herry Purnomo

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