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All Journal Elkawnie Agrosains: Jurnal Penelitian Agronomi Agrotechnology Research Journal Jurnal Agrotek Lestari Jurnal Agrium
Oviana Lisa
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Syiah Kuala Darussalam, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering

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Identification and Phylogenetic Analysis of Entomopathogenic Fungal Isolates Using Molecular Approach Syaukani Syaukani; Zulia Ananda; Suhartono Suhartono; Sirtina Sirtina; Oviana Lisa; Alfizar Alfizar; Samingan Samingan
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 2 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.6549

Abstract

Abstract: Entomopathogenic fungi are essential to consider as one of the biological agents to control termite populations. This research aimed to molecularly identify entomopathogenic fungi isolates in termites based on the ITS rDNA region and to determine the relationship of fungi isolates. Identification was performed by DNA extraction, PCR amplification, electrophoresis, purification, and sequencing. Phylogenetic trees were generated using MEGA X.  Molecular identification showed that the ISO1 sample was Penicillium oxalicum, the ISO2 sample was Trichoderma ghanense the ISO3 sample was Aspergillus niger, the ISO4 sample was Aspergillus fumigatus and the ISO5 sample was Aspergillus pseudonomius. The phylogenetic tree showed that the ISO1, ISO2, ISO3, ISO4, and ISO5 samples had the closest relationship with Penicillium oxalicum strain FR6-CGR12, Trichoderma ghanense isolate TM2, Aspergillus niger isolate 77, Aspergillus fumigatus, and Aspergillus pseudonomius strain DTO 267D6, respectively.Abstrak: Kelimpahan jenis fungi entomopatogen adalah hal yang terpenting untuk dipertimbangkan sebagai agen hayati bagi populasi rayap.Penelitian ini bertujuan untuk mengidentifikasi secara molekular isolat fungi entomopatogen pada rayap, berdasarkan daerah ITS rDNA dan mengetahui hubungan kekerabatan dari isolat fungi tersebut. Identifikasi dilakukan dengan cara ekstraksi DNA, amplifikasi menggunakan PCR, elektroforesis, purifikasi dan sekuensing. Selanjutnya kontruksi pohon filogenetik menggunakan aplikasi MEGA X. Berdasarkan uji molekular menunjukkan bahwa sampel ISO1 merupakan Penicillium oxalicum.ISO2 merupakan Trichoderma ghanense.ISO3 merupakan Aspergillus niger. ISO4 merupakan Aspergillus fumigatus.ISO5 merupakan Aspergillus pseudonomius.Konstruksi pohon filogenetik menunjukkan bahwa, sampel ISO1 berkerabat dekat dengan Penicillium oxalicum strain FR6-CGR12. Sampel ISO2 berkerabat dekat dengan Trichoderma ghanense isolat TM2. Sampel ISO3 berkerabat dekat dengan Aspergillus nigerisolat 77. Sampel ISO4 berkerabat dekat dengan Aspergillus fumigatus.Sampel ISO5 berkerabat dekat dengan Aspergillus pseudonomius strain DTO 267D6.
Identification of Morphology and Molecular PCR-RAPD Bactrocera spp. in the Location of Red Guava Crops, Deli Serdang District Putri Mustika Sari; Oviana Lisa; Lisdayani Lisdayani
Agrotechnology Research Journal Vol 6, No 2 (2022): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.692 KB) | DOI: 10.20961/agrotechresj.v6i2.67181

Abstract

Various fruit flies are in Indonesia, but only a few species of fruit flies have been found at the red guava plant in Deli Serdang District. Knowing the species of fruit flies are needed to do the proper control. In addition to morphological identification, it is necessary to carry out molecular characterization to obtain accurate results in characterizing species differences. This study aims to identify the fruit fly based on morphology and molecularly PCR-RAPD for mapping the genetic closeness of the relationship between individual fruit flies. Bactrocera morphologically identified at LIPI, namely B. carambolae, B. papayae, B. caudata, B. albistrigata, B. umbrosa, B. curcubitae, B. tau, and B. kinabalu. For molecular identification PCR-RAPD, shows the dendrogram results from the Neighbor-Joining analysis based on RAPD markers of DNA band characters showing the genetic proximity of eight species divided into three groups of Bactrocera based on their location. The closeness of genetic relationships between individuals was analyzed using Pairwise Distance Calculation which describes the genetic distance between species. The results of Pairwise Distance Calculation ranged from 0.13-0.42. By knowing what species there fruit flies are in red guava plantations in the Deli Serdang district, it can make it easier for farmers to monitor and control fruit flies in red guava plantations.
Penggunaan Buah Maja sebagai Pupuk Organik dan NPK Mutiara terhadap Pertumbuhan Kacang Hijau di Kabupaten Serdang Bedagai Putri Mustika Sari; Mawaddah Putri Arisma Siregar; Oviana Lisa
Agrosains : Jurnal Penelitian Agronomi Vol 24, No 2 (2022): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v24i2.65338

Abstract

Indonesia has experienced a decline in green bean production due to unsuitable green bean cultivation and low soil fertility. However, combining NPK Mutiara and organic fertilizer made from active maja fruit containing NPK Mg and Fe can increase soil fertility and quality in an environmentally friendly way. This study aims to examine the effect of combining organic fertilizers with active ingredients of maja fruit and pearl NPK in increasing the growth of green bean varieties of VIMA-1. The research was carried out in Sukajadi Village, Perbaungan, Serdang Bedagai District, from October to December 2021 using a 2-factor RAK design where the first factor was PO application with four treatment levels, namely M0 = Control; M1 = 100ml PO: 750 ml water; M2 = 100 ml PO: 1500 ml water; M3 = 100 ml PO: 2250 ml water. Then the second factor is the dose of NPK Mutiara fertilizer where P1 = 30g/plot and P2 = 40g/plot with the number of replications 3. The results showed that the administration of PO with the active ingredient of maja fruit had an insignificant effect on all parameters observed. However, the NPK fertilizer treatment had a significant impact on diameter. Stems at treatment P1 = 30g/plot.
Virulensi Cendawan Entomopatogen Aspergillus spp.Sebagai Agen Biokontrol Hama Rayap Coptotermes curvignathus (Isoptera: Rhinotermitidae) Oviana Lisa; Syaukani Syaukani; Lenni Fitri; Putri Mustika Sari; Siti Aminah; Ernilasari Ernilasari
Jurnal Agrotek Lestari Vol 9, No 1 (2023): April
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/jal.v9i1.7253

Abstract

The termite Coptotermes curvignathus is an important pest in oil palm plantations and environmentally friendly control measures are needed. The fungus Aspergillus spp. has potential as a biocontrol agent against the termite C. curvignathus. The purpose of this study aimed to obtain Aspergillus spp. which are virulent against C. curvignathus. Aspergillus spp. isolates used in this study were collected from termite nests in the primary forest at Suaq Balimbing Research Station, Gunung Leuser National Park, Sumatra. This study used an experimental method with a completely randomized design (CRD). Two isolates of Aspergillus spp. each 105 conidia/mL were applied to worker caste termites. The results of the virulence assay showed that isolate Aspergillus sp. 1 had the highest causing 82.13% mortality of C.curvignathus and with  an LT50 value of 4.18 days.
Kombinasi Pemupukan Organik dan Anorganik Terhadap Laju Pertumbuhan dan Rasio Bobot Basah Tajuk Akar Tanaman Padi Sawah (Oryza sativa L.) Siti Aminah; Muhammad Afrillah; Oviana Lisa; Muhammad Reza Aulia
Jurnal Agrotek Lestari Vol 9, No 1 (2023): April
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35308/jal.v9i1.7780

Abstract

Rice as one of the main food sources for the Indonesian people, especially North Sumatra, is still not able to meet the needs even though it has large land potential, so it is necessary to increase production through fertilization. This study aims to determine the effect of a combination of organic and inorganic fertilization on the growth of paddy rice plants. This study used a factorial randomized block design consisting of three factors, namely KCL (K) at 4 dose levels, namely K0 (0 kg/ha), K1 (25 kg/ha), K2 (50 kg/ha) and K3 (75 kg/ha). ), Urea (U) two levels namely U1(250 kg/ha) and U2 (225 kg/ha), Straw Compost (J) 2 levels namely J0 (0 tons/ha) and J1 (5 tons/ha) with a total The treatment combinations were 16 and consisted of 3 replications and 48 samples. Held in the rain-fed rice field area of Clove Turi sub-district, Binjai City. Soil sample analysis was carried out at the Analytical Laboratory of PT. Socfin Indonesia (SOCFINDO), Medan City and the Research and Technology Laboratory, Faculty of Agriculture, University of North Sumatra. The combination of inorganic and organic fertilizer treatments consisting of Potassium, Urea and Straw Compost showed no significant effect on the observed parameters of plant growth rate (cm/week) and root canopy wet weight ratio (grams). The highest plant growth rate was shown by the interaction of the three treatments, namely K3U2J0 of 10.33 cm/week, while the lowest data was shown by the interaction of the three treatments, namely K1U2J0 of 8.87 cm/week. The highest root canopy wet weight ratio was shown by the interaction of the three treatments, namely K1U1J0 of 8.69 grams while the lowest data was shown by the interaction of the two treatments between potassium and straw compost, namely K2J0 of 5.01 grams.Keywords: Fertilizer; Organic; Inorganic; Growth; Paddy
Evaluasi Keragaan Hasil Dan Skoring Keparahan Penyakit Karat Daun Pada Tanaman Sorgum Dewi Andriani; Siti Aminah; Rayhan Amadius Weihan; Ovianalisa Ovianalisa
Agrium Vol 20, No 2 (2023)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v20i2.11444

Abstract

Sorgum (Sorghum bicolor L.) merupakan tanaman pangan fungsional dan alternatif sebagai pengganti beras dan gandum karena mengandung gizi unik dan bebas gluten. Penelitian ini bertujuan untuk mengetahui keragaan hasil dan tingkat keparahan serta kriteria ketahanan penyakit karat daun pada galur F6 sorgum. Penelitian menggunakan Rancangan Augmented yang terdiri dari 20 galur F6 sorgum tanpa ulangan dan 5 varietas pembanding sebagai kontrol dengan 3 ulangan sehingga diperolah 35 satuan percobaan. Data dianalisis dengan uji F dan dilanjutkan dengan Uji BNT pada taraf 5%, kemudian dilanjutkan dengan perhitungan nilai heritabilitas dan korelasi pearson. Hasil penelitian menunjukkan bahwa nilai heritabilitas setiap karakter yang diamati tergolonng tinggi. Tingkat keparahan penyakit karat daun berkorelasi negatif terhadap bobot malai dan bobot biji malai-1  yang menunjukkan adanya tingkat penyakit rendah dengan bobot hasil yang tinggi. Terdapat 7 galur diantaranya A-19-18-3, A-21-14-3, A-21-14-2, A-16-21-5, B-15-25-3, B-1-24-2 dan B-4-6-7 yang memiliki bobot hasil yang baik dan tahan terhadap penyakit karat daun.
Co-Authors Alfizar Alfizar Dewi Andriani Ernilasari Ernilasari Lenni Fitri Lisdayani, Lisdayani Mawaddah Putri Arisma Siregar Muhammad Afrillah Muhammad Reza Aulia Putri Mustika Sari Rayhan Amadius Weihan Samingan Samingan Sirtina Sirtina Siti Aminah Siti Aminah Suhartono Suhartono Syaukani Syaukani Syaukani Syaukani Zulia Ananda