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Mashuri Masri
Department of Biology, Science and Technology Faculty, State Islamic University Alauddin, Makassar
Biochemistry, Genetics & Molecular Biology Education Engineering

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Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB Mashuri Masri; Cut Muthiadin; Masita Masita; Tri Cahyanto; Lianah Lianah; Rusny Rusny; Siska Tridesianti
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.9335

Abstract

Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of  N. sativa seed extract in inhibiting the growth of  M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan.  Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua  strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%,  tetapi resisten terhadap  ekstrak N. sativa konsentrasi 1 dan 3%.
The Potency of Bioplastic Polyhydroxyalkanoate (PHA) Producing Bacteria Isolated From Palm Oil Mill Waste Nur Haedar; Donny Suherman; Zasarwati Dwyana; Heriadi Heriadi; Mashuri Masri
Elkawnie: Journal of Islamic Science and Technology Vol 8, No 1 (2022)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v8i1.11145

Abstract

Abstract:  Polyhydroxyalkanoate (PHA) is a polymer made up of biodegradable plastics that can be synthesized by microorganisms from various substrates that contain lots of carbon sources including fatty acids and sugars. One of the substrates that contain a lot of carbon sources is waste from palm oil processing plants. PHA-producing bacteria can take advantage of excess carbon sources in palm oil waste in the form of fatty acids to be converted into PHA. This study aims to determine the potential of bacteria isolated from palm oil mill waste to produce polyhydroxyalkanoates (PHA) and to determine the optimum time required for bacteria to produce PHA. Optimization of the fermentation time was carried out at 24, 48, and 72 hours using minimum Ramsay media added with 1% palm oil and 1% glucose. The results of the study obtained 20 isolates of bacteria isolated from palm oil mill waste, and 9 of them were able to produce polyhydroxyalkanoate (PHA) qualitatively. The results of quantitative selection obtained 2 bacterial isolates capable of producing PHA, namely isolates CPS 3 and LPS2 CPS 3, with crotonic acid absorbance values of 41.6 and 5.01, respectively with a fermentation time of 72 hours. Based on the results of genotypic identification using 16S rRNA DNA sequences, isolates of CPS 3, including Bacillus sp. strain CL33 and isolate LPS 2, belonged to Bacillus flexus strain S5a. This shows that isolates from palm oil mill waste can be used as a source of isolate for PHA production.Abstrak: Polyhydroxyalkanoate (PHA) merupakan polimer penyusun plastik biodegredable yang dapat di sintesis oleh mikroorganisme dari bermacam-macam substrat yang banyak mengandung sumber karbon asam lemak dan gula. Salah satu subtrat yang banyak mengandung sumber karbon adalah limbah yang berasal dari pabrik pengolahan kelapa sawit. Bakteri penghasil PHA dapat memanfaatkan sumber karbon berlebih dalam limbah kelapa sawit berupa asam-asam lemak untuk diubah menjadi PHA. Penelitian ini bertujuan untuk mengetahui potensi bakteri yang diisolasi dari limbah pabrik kelapa sawit dalam menghasilkan Polyhydroxyalkanoate (PHA) serta mengetahui waktu optimum yang dibutuhkan oleh bakteri dalam menghasilkan PHA. Optimasi waktu fermentasi dilakukan pada 24, 48 dan 72 jam menggunakan media minimal Ramsay yang ditambahkan minyak sawit 1% dan glukosa 1%. Hasil penelitian memperoleh 20 isolat bakteri yang diisolasi dari limbah pabrik kelapa sawit dan 9 isolat diantaranya mampu menghasilkan Polyhydroxyalkanoate (PHA) secara kualitatif. Hasil seleksi secara kuantitatif diperoleh 2 isolat yang mampu menghasilkan PHA yaitu isolat CPS 3 dengan nilai absorbansi asam krotonoat sebesar 41,6 sedangkan LPS 2 memiliki nilai absorbansi asam krotonoat sebesar 5,01 dengan waktu fermentasi selama 72 jam. Berdasarkan hasil identifikasi secara genotipik dengan menggunakan sekuens DNA 16S rRNA isolat CPS 3 termasuk jenis Bacillus sp. strain CL33 dan isolat LPS 2 termasuk jenis Bacillus flexus strain S5a. Hal ini menunjukkan bahwa isolat dari limbah pabrik kelapa sawit dapat digunakan sebagai sumber isolat untuk produksi PHA.
Co-Authors Cut Muthiadin Donny Suherman Heriadi Heriadi Lianah Lianah, Lianah Masita Masita Nur Haedar Rusny Rusny Siska Tridesianti Tri Cahyanto Zasarwati Dwyana