Nanik Indah Setianingsih
Balai Besar Teknologi Pencegahan Pencemaran Industri

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Low Energy Bacteria Preservation of Extremely Halophilic Archaea Haloferax Lucentense and Haloferax Chudinovii Immobilized using Natural Zeolite Rizal Awaludin Malik; Nilawati Nilawati; Novarina Irnaning Handayani; Rame Rame; Silvy Djayanti; Ningsih Ika Pratiwi; Nanik Indah Setianingsih; Nasuka Nasuka
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 2 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no2.p16-28

Abstract

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
Influence of Operational Condition on the Performance of Halotolerant Enriched - Activated Sludge System for Treating Medium Salinity Peanut Roasted Wastewater Rustiana Yuliasni; Nanik Indah Setianingsih; Kukuh Aryo Wicaksono; Nani Harihastuti
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 2 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no2.p46-54

Abstract

This research aimed to investigate the influence of operational condition on the performance of halotolerant enriched - activated sludge system for treating high organic wastewater with medium salinity from roasted peanut industry. Roasted peanut wastewater with VLR ranged from 0.268 to 4.7 kg COD/m3.day and Chloride concentration ranged between 1582 - 4392 mg/L was treated continuously for almost 77 days. Two identical reactor with Volume 25 L, namely R1 a conventional Activated Sludge (AS) System and R2, a halotolerant enriched-AS. Both reactors were running with operational condition: HRT (9 h to 46 h) and MLSS (1000-6000 mg/L). Compared to conventional AS system, Halotolerant enriched-Activated sludge system could remove average of 86.7% COD, compared with conventional AS which was 85.7%. Average COD effluent of Halotolerant Enriched-Activated Sludge was also considerably lower, which was 127 mg/L, than conventional AS which was 150 mg/L. Halotolerant enriched-activated sludge also produced less sludge, giving a high F/M ratio (4.9) compared with conventional AS (3.5). In order to make effluent fulfilled stream standard regulation (at central java region COD was<150 mg/L), the favorable operational condition for both reactors would be at VLR 0.268 to 2.03 kg COD, HRT was 25 hours HRT, with MLSS was 2584 – 3956 mg/L and maximum chloride concentration 1920 mg/L.
Pengolahan Air Limbah Industri Bakery Menggunakan Sistem Stripper-Lumpur Aktif Rizal Awaludin Malik; Hanny Vistanty; Sartamtomo; Nanik Indah Setianingsih; Farida Crisnaningtyas; Nur Zen
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 7 No. 2 (2016)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2016.v7.no2.p89-98

Abstract

Air limbah industri bakery memiliki karakteristik kandungan organik, lemak, dan surfaktan yang tinggi. Keberadaan surfaktan pada air limbah dapat mengganggu proses degradasi kandungan organik dan lemak pada instalasi pengolahan air limbah khususnya pengolahan secara biologis/lumpur aktif. Studi ini membahas tentang performa integrasi system stripper-lumpur aktif yang diaplikasikan untuk mengolah air limbah industry bakery dalam skala full scale. Proses stripper dilakukan dengan menggunakan kolom yang diintegrasikan dengan bak aerasi pada kisaran HRT (waktu tinggal) 24 jam. Proses stripper memberikan efisiensi enurunan COD dan surfaktan tertinggi sebesar 96% dan 80%. Proses lumpur aktif digunakan untuk mengolah keluaran dari proses stripper dengan karakteristik lumpur aktif MLSS 4000-4500 mg/L, FM/ rasio 0,268, sludge age 11 hari dan HRT 24 jam, proses degradasi dengan lumpur aktif ini mampu memberikan efisiensi penurunan COD dan surfaktan sebesar 79% dan 88%. Hasil penelitian menunjukkan bahwa unit IPAL dengan menggunakan sistem stripper-lumpur aktif memiliki efisiensi penurunan COD, TSS, dan BOD sebesar 98,9%, 87,9%, dan 96,4%, sehingga sistem ini merupakan teknologi yang tepat untuk diterapkan pada pengolahan limbah industri bakery.
Perencanaan Bak Pengendap pada Sistem Lumpur Aktif dengan Metode Grafis Nanik Indah Setianingsih; Djarwanti; Moch. Syarif Romadhon
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 7 No. 2 (2016)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2016.v7.no2.p99-104

Abstract

Pengendapan merupakan suatu cara yang paling sederhana, murah, dan banyak digunakan dalam proses pengolahan air limbah. Kinerja dari suatu design bak pengendap sangat spesifik untuk setiap jenis limbah, oleh sebab itu jika suatu peralatan sedimentasi dirancang tanpa suatu eksperimen, kinerja yang dihasilkan sering tidak memuaskan. Metode grafis merupakan salah satu cara dalam menentukan desain bak sedimentasi. Metode grafis dapat menghasilkan suatu desain bak pengendap yang tepat sebagaimana sistem komputasi karena ditentukan dengan eksperimen namun dengan cara yang lebih sederhana dan biaya yang murah. Tujuan dari penelitian ini adalah merancang bak pengendap air limbah dari proses lumpur aktif dengan metode grafis disesuaikan dengan kriteria dasar-dasar perencanaan bak pengendap. Penelitian secara laboratoris dilakukan melalui pengamatan proses pengendapan dalam tabung kaca tinggi 150 cm dan luas penampang 100 cm2. Parameter yang diamati adalah tinggi endapan vs waktu pengendapan. Dengan perhitungan secara grafis didapatkan hasil sebagai berikut. Untuk mengendapkan lumpur dengan kandungan TSS awal 4325 mg/L menjadi 18.000 mg/L dengan debit 300 m3/hari diperlukan HRT (Hydraulic Retention Time) 1,87 jam, Luas area pengendapan 20,83 m2, Volume bak pengendap 31,24 m3. Perkiraan lumpur yang dihasilkan adalah sebesar 98,61 kg TSS/hari.
KEMAMPUAN BAKTERI HALOFILIK UNTUK PENGOLAHAN LIMBAH INDUSTRI PEMINDANGAN IKAN Nilawati Nilawati; Marihati Marihati; Susdawanita Susdawanita; Nanik Indah Setianingsih
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 6 No. 1 (2015)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2015.v6.no1.p23-28

Abstract

Industri pemindangan ikan dalam proses pengolahannya menghasilkan limbah cair. Limbah tersebut langsung dibuang ke sungai, hal ini dapat menyebabkan pencemaran lingkungan terutama bau akibat dari pembusukan protein.Kapasitasnya 150 m3 per-hari untuk 1 industri. Limbah cair rebusan ikan pindang mengandung beban cemaran yang cukup tinggi, dengan nilai permanganat 15.073 ppm dan BOD 5.380 ppm.Pengolahan limbah cair dalam penelitian ini menggunakan sistem batch dan kontinyu yang diamati setiap hari selama 8 hari (hari ke 0, 1, 2, 3, 4, 5, 6 dan 7). Hasil penelitian menunjukkan penurunan nilai permanganat paling optimal terjadi pada hari ke 6 sebesar 4.266 ppm atau penurunannya sebesar 2.401 ppm , atau 36,01 persen untuk sistem batch. Sistem kontinyu penurunan optimal pada hari ke-3 yaitu 5.906 ppm turun 13,47 persen.Jadi sistem batch lebih efektif daripada sistem kontinyu pada pengolahan limbah pindang dengan bakteri halofilik. Nilai BOD terjadi penurunan yang signifikan, untuk pengolahan dengan sistem batch secara keseluruhan nilai BOD sistem batch lebih rendah daripada sistem kontinyu. Penurunan yang optimal pada hari ke-6menjadi 496 ppm terjadi penurunan sebesar 1.203 ppm atau 70,81 persen, sedangkan sistem kontinyu nilai BOD tidak terjadi penurunan.
Performance of Immobilized-Selected Microorganisms in The Biodegradation of Textile Industry Waste Water Novarina Irnaning Handayani; Nanik Indah Setianingsih; Misbachul Moenir
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 9 No. 1 (2018)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2018.v9.no1.p29-37

Abstract

Waste water from textile industry contain pollutant whit in certain concentration. To protect the environment and water bodies, wastewater containing pollutant must be treated before discharging into the environment. Anaerobic biological treatment has been used as a method in treating textile industry waste water. Several factors of conventional anaerobic treatment needs to be repaired in order to improve the performance of treating wastewater. A selected microorganisms as inoculum was expected to increase effectivity of waste water biodegradation. In this study a selected microorganisms was used as inoculum in the form of immobilized and free cells in anaerobic treatment of waste water from textile industry and compared with conventional sludge. Results from this study show that selected-immobilized microorganisms achieved the best performance due to its stability and efficiency in removing pollutant in the waste water. Mean while microorganisms in the form of free cells got the lowest performance in treating waste water, estimated due to its sensitivity of environmental conditions and having low mechanical strength of biomass. Immobilized cells succesfully treated waste water from textile industry, removal of pollutant in suspended solid parameter reached on 93,78% mean while, in oil & grease, BOD5 and COD parameter reached on 99,13%, 81,54% and 64,94% respectively. Pollutant in amonia parameter has not been fully treated in this experiment due to condition of the experiment system was anaerobic instead of aerobic.
Pengolahan Air Limbah Kadar Garam Tinggi dengan Sistem Lumpur Aktif Nanik Indah Setianingsih; Danny Widyakusuma Hermawan; Nilawati Nilawati
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 6 No. 2 (2015)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2015.v6.no2.p45-50

Abstract

Air limbah perebusan dari industri kacang garing masih menjadi permasalahan karena mengandung garam yang tinggi. Pada kegiatan penelitian ini dilakukan pengolahan air limbah tersebut dengan sistem lumpur aktif dengan memanfaatkan mikroba yang sudah teradaptasi dengan kadar garam tinggi berasal dari bak equalisasi air limbah tersebut. Percobaan penelitian pengolahan limbah dilakukan dengan dua kondisi yaitu kadar MLVSS (Mixed Liquour Volatile Suspended Solid) awal 1000 mg/L dan 2000 mg/L, pengamatan sampel dilakukan setiap 24 jam selama lima hari. Hasil analisis menunjukkan bahwa terdapat penurunan nilai BOD dan permanganat pada sampel air limbah namun tidak terjadi penurunan untuk parameter nilai klorida. Penurunan nilai BOD adalah sebesar 89,89% dengan perlakuan MLVSS 1000 mg/L dan 91,01 % untuk perlakuan MLVSS 2000 mg/L. Kondisi optimal pada sistem lumpur aktif belum tercapai yang ditunjukkan dari nilai sludge volume masih kurang dari 30%. Sampel hasil pengolahan belum dapat memenuhi baku mutu yang dipersyaratkan dengan nilai BOD 352,2 mg/L, permanganat 213,4 mg/L dan klorida 8347 mg/L.
Evaluating the Performance of Three Chambers Microbial Salinity Cell (MSC) Subjected to Different Substrate Concentrations to Accomplish Simultaneous Organic and Salt Removal in the Wastewater Rustiana Yuliasni; Nur Zen; Nanik Indah Setianingsih
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 11 No. 1 (2020)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2020.v11.no1.p19-26

Abstract

This study aimed to identify the effect of substrate concentration on the performance of A Three chambers Microbial Salinity Cell (a three chambers MSC). In this study, 3 three chambers MSC was made of plexy glass with total volume of 200 ml. Alumunium wrapped with with platinum on vulcan carbon cloth were used as electrodes,with each working area 63 cm2. The results showed that a Three chambers Microbial Salinity Cell was able to generate electricity and at the same time removed salinity. The degree of electricity deneration and salinity removal were influenced by initial substrate concentration in the anode chamber. The higher substrate concentration, the better performance of MSC. The best performance of MSC achieved when COD was 2034 mg/L, resulted in maximum voltage of 0. 44 V, and maximum current density of 0.29 mA/m2. With % CE was 5.4%. The maximum conductivity increase in salinity chamber was from 11.2 µS/cm to 1027 µS/cm (salinity 0.57% ppt)
Full Scale Application of Integrated Upflow Anaerobic Filter (UAF)-Constructed Wetland (CWs) in Small Scale Batik Industry Wastewater Treatment Novarina Irnaning Handayani; Rustiana Yuliasni; Nanik Indah Setianingsih; Agung Budiarto
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 11 No. 1 (2020)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2020.v11.no1.p27-35

Abstract

This research aimed to evaluate the implementation of integrated Upflow Anaerobic Filter (UAF)-Constructed Wetlands (CWs) in real condition of wastewater treatment plant in batik small scale industry. The full scale reactor consisted of equalization chamber with HRT of 2 days; Upflow Anaerobic Filter (UAF) with HRT of 6 days, and Horizontal Subsurface Constructed Wetlands (HSSCWs) with HRT of 1.5 days. The UAF- CWs integrated technology was used to treat batik wastewater with COD inlet of 1339 – 2034 mg/L and pH of 9.0 – 9.4. This study showed that single UAF technology alone was able to reduced 56 – 78%, while the integration UAF –wetland technology improved the performance to 85% and reduced the pH into 7.5 – 7.8
Biogas Production from Sugarcane Vinasse: A Review Rustiana Yuliasni; Rieke Yuliastuti; Nanik Indah Setianingsih
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 12 No. 2 (2021)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2021.v12.no2.p34-44

Abstract

Biogas is a renewable energy sources that could replace the role of fossil fuel. Biogas could be produced from biomass or agro-industrial wastewater. Sugarcane vinasse has potential of biogas production due to its high BOD concentration (10–65 g BOD/l). However, the biogas production from sugarcane vinasse has several drawbacks that hinders the maximum biogas yield, such as: acidic pH (pH 3.5 – 5.0), high temperature (80–90°C) and high concentration of sulfuric acid (> 150 mg/L). Theoretically, the methane potential per gram COD is 0.35 L/gr COD, containing of 60% methane. However, up to date, the maximum biogas production from vinasse was less then its theoretical value. To get the full potential of biogas production from vinasse wastewater as well as to reduce the capital cost for full scale application, combination of suitable pre-treatment, selected microorganisms and bioreactor design-configuration are the most important parameters to be considered. This paper aims to explore the potential of sugarcane vinasse to produce biogas, by elaborating the aforementioned key parameters. In this review the basic characteristic and the potency of sugarcane vinasse wastewater will be elaborated.  Furthermore, the effect of key parameters such as pH, temperature, and organic load to biogas production will also be discussed. The biogas technology will also be explored. Lastly, conclusion will be determined