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All Journal Jurnal Riset Teknologi Pencegahan Pencemaran Industri
Ningsih Ika Pratiwi
Balai Besar Teknologi Pencegahan Pencemaran Industri
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science

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Low Energy Bacteria Preservation of Extremely Halophilic Archaea Haloferax Lucentense and Haloferax Chudinovii Immobilized using Natural Zeolite Rizal Awaludin Malik; Nilawati Nilawati; Novarina Irnaning Handayani; Rame Rame; Silvy Djayanti; Ningsih Ika Pratiwi; Nanik Indah Setianingsih; Nasuka Nasuka
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 2 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no2.p16-28

Abstract

The methods of microbial cells preservation were already known by liquid drying, freeze-drying, and freezing. Those methods could preserve bacteria cells in a long period of time but its survivability was relatively low and used relatively high energy during preservation. Immobilization was known as entrapping, attaching or encapsulating bacterial cells in a suitable matrix. This research was conducted to know the suitability of zeolite as immobilization carrier and also as preservation matrix of two halophilic archaea Haloferax chudinovii and Haloferax lucentense. Variable of this research was the type of the carrier which was raw zeolite, 110oC and 300oC heat-activated zeolite carrier, parameters measured in this study was physical and chemical of zeolite such as chemical content, Si/Al ratio, surface area and pore volume, and biochemical assay, bacterial cells numbers after immobilization and bacterial cells after preservation as bacterial response to the immobilization and preservation. Heat activation was significantly affecting the chemical composition, carrier surface area, and pore volume. Highest surface area, pore volume, and Si/Al ratio were obtained in 110oC pretreated zeolite followed by 300oC pretreated zeolite. The bacterial cells obtained after immobilization process was 1,8x107 cfu/g, 3,0 x 107 cfu/g, and 2,1x107 for raw zeolite, 110oC pretreated zeolite and 300oC zeolite respectively. After 4 months preservation, the slight reduction of the bacterial cells was observed. Immobilization halophilic archaeae using zeolite as carrier was proven as low cost and effective preservation method due to relatively simple process and unspecific preservation temperature requirements.
Adsorption And Regeneration Of Sungai Pasak Pumice As An Adsorbent For Ammonium Removal In Water Ningsih Ika Pratiwi; Annisa Huwaida; Shinta Indah; Denny Helard
Jurnal Riset Teknologi Pencegahan Pencemaran Industri Vol. 10 No. 1 (2019)
Publisher : Balai Besar Standardisasi dan Pelayanan Jasa Pencegahan Pencemaran Industri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21771/jrtppi.2019.v10.no1.p38-46

Abstract

Pumice is known as a low-cost material with a porous structure, has the potential as an adsorbent to remove various kinds of pollutant compounds. The abundance of pumice is found in Sungai Pasak area and it never been utilized. This study was performed to see the ability of Sungai Pasak pumice as an adsorbent for ammonium removal in water along with its ability to be regenerated. The study was carried out triplo in batches to obtain optimum adsorption conditions which were then used in desorption experiments using HCl 0.1 M agent for regeneration process. The optimum conditions obtained: adsorbate pH 6, adsorbent dose 0.3 g/l, contact time 30 minutes, adsorbent diameter <63 µm and adsorbate concentration 4 mg/l. The results showed that pumice has ability to remove ammonium with adsorption capacity at optimum conditions were 47.06% and 6.27 mg/g with Freundlich's isotherm equation (R2=0.997). Acid agents are able to adsorb ammonium from pumice with an average desorption percentage 88.89% after 2 times of reuse, proving that acidic agents are able to regenerate pumice adsorbents so they can be reused. The potential for adsorption and regeneration of the Sungai Pasak pumice can be utilized to treat water with ammonium such as groundwater and waste water.
Co-Authors Annisa Huwaida Denny Helard Nanik Indah Setianingsih Nasuka Nasuka Nilawati Nilawati Novarina Irnaning Handayani Rame Rame Rizal Awaludin Malik Shinta Indah Silvy Djayanti