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All Journal Jurnal Perlindungan Tanaman Indonesia
Susamto Somowiyarjo
Faculty of Agriculture Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry

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Propagation and Purification of Baculovirus oryctes Huger Susamto Somowiyarjo; YB Sumardiyono; Sedyo Hartono; Triharso Triharso; Jun Kobayashi
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4408.918 KB) | DOI: 10.22146/jpti.9317

Abstract

An isolate of Baculovirus oryctes, a possible biological control agent for coconut beetle (Oryctes rhinoceros Huger) from East Java was propagated and purified. The virus could be transmitted by feeding the imago with 10% sucrose containing virus from homogenate of infected beetles. Effectivity of virus to 9 healthy females by sexual copulation. Virus be succesfully purified by a method of Payne.
Properties of Oryctes Baculovirus Isolated in Indonesia Jun Kobayashi; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7632.018 KB) | DOI: 10.22146/jpti.9318

Abstract

An Indonesian isolate of Oryctes baculovirus was purified from infected midguts of the rhinoceros beetle (Oryctes rhinoceros) by centrifugation on a 10–40% (w/v) sucroese gradient. Morphological features of  nucleocapsid including a tail-like projection were very same as those previously reported. Both protein components of purified particles and restriction fragment electrophoresis profiles of viral DNA were similar to those of their isolates of Oryctes baculovirus, although there were some differences.
Co-Authors Jun Kobayashi SEDYO HARTONO Triharso Triharso YB Sumardiyono