I. Hartana
Asosiasi Penelitian Perkebunan Indonesia

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Pengendalian Hayati Penyakit Layu Bakteri Tembakau: 2. Percobaan di Rumah Kaca Triwidodo Arwiyanto; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 1 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9967

Abstract

Tobacco bacterial wilt caused by Ralstonia solanacearum has been controlled biologically in the greenhouse by the use of rhizosphere microorganisms, i.e. fluorescent pseudomonads and Bacillus spp. Root dipping for 30 minutes in the antagonist suspension (108 cfu/ml) suppressed bacterial wilt development. The best results were obtained with fluorescent pseudomonads Pf-20 and Bacillus sp. ba-118 isolates but the repression was annulled with the use of its combination. Disease severity were higher when the plants were treated with the combination between fluorescent pseudomonads. Treatment combinations between Pf-28, Pf-31, Pf-33, and Ba-118 could reduce the disease index.
Deteksi Strain Pseudomonas solanacearum Penghasil Bakteriosin Triwidodo Arwiyanto; Sudarmadi Sudarmadi; I. Hartana
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 2 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12890

Abstract

The capability of Pseudornonas solanacearum to produce bacteriocin was investigated with theuse oj Casamino-acid Peptone Glucose Agar. All strains tested produce bacteriocm withvarious spectra. Strain 6, 36 and ./9 inhibited almost all of the other strains. The avirulenttype of those three strains. i.e. 6-op. 36-op and -19-op, still produced bacteriocln on the samemedium. The.concentratlon of carbon source and acidity of the medium did not have any effecton the bacteriocin production. The bacterioctn produced by strains 6-op, 36-op and 49-opwere inactivated at 70°C for 30 minutes.