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Sri Noegrohati
Departement of Chemistry Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
Medicine & Pharmacology

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CLEANING EFFECTIVITY OF SEVERAL SURFACTANS TO PESTISIDES RESIDUES ON FRESH APEL FRUITS Endang Lukitaningsih; B.S. Ari Sudarmanto; Sri Noegrohati
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (65.324 KB) | DOI: 10.14499/indonesianjpharm0iss0pp200-206

Abstract

Intensification efforts in farming to increase productivity must consider the pesticide utilization, especially insecticide and herbicide. Several pestisides which are still used include carbofuran and organochlorine, some of them have lipofilic properties and might harm to human health. Therefore, an effort is required to washing off pesticides from farming products is one of the effort which can be performed. Since pesticides has lipofilic properties, therefore cleaning pesticides with water is not sufficient. Surfactant is required to increase washing off ability of water. Wash off ability of several surfactants circulated on the market i. e. SL, ML and A were investigated. The result showed that the wash off ability values of surfactants to DDT residues on fresh apples were 79.18 %, 75.19 % and 67.49 % for SL, A and ML respectively. The wash off effectiveness of surfactant A, SL and ML to -metrine were 85.29 %, 80.48 % and 64.47 % respectively.Key words: pesticide, cleaning efficiency surfactant, DDT, -metrine.
ESTIMATION OF PROTEIN CONCENTRATION IN FOOD BY TOTAL NITROGEN AND AMINO ACID ANALYSES Sumarno .; Sri Noegrohati; Narsito .; Iip Izul Falah
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.079 KB) | DOI: 10.14499/indonesianjpharm0iss0pp34-43

Abstract

Since protein is the principal constituent of biological organs, a continuous supply is needed in food for growth and repair. Protein quality in food is determined by its assortment of amino acids, whether it is essentially needed or not. The aim of this study is to develop an accurate and precise simple method of protein estimation for food and feed. The validity of simple protein estimation by multiplying the N-total concentration with a constant factor: of 6.25, should be clarified. Protein could be estimated more specifically by summing up the determined amino acids. In this study, the amino acids of some substantially high protein food were analysed by HPLC-ninhydrin, HPLC-o-phthalaldehyde, and HPLC-dansyl techniques, by gas chromatograph after derivation with TFA-butanol, and by formol titration. Method based on HPLC-ninhydrin techniques shows significant correlation (r=0-9992) with its amino acids content and N-total content. The protein as amino acids - N-total conversion factor obtained was 6.95 for N-total content less than 15.5%, and 8.59 for N-total content higher than 15.5%.Key words: food, protein and analytical methods
Co-Authors B.S. Ari Sudarmanto Endang Lukitaningsih Iip Izul Falah Narsito . Sumarno .