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All Journal Research Journal of Life Science Samakia: Jurnal Ilmu Perikanan
Suleman Suleman
Aquaculture Department, Faculty Of Fisheries And Marine Science, University Of Brawijaya
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other

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Potensi Ekstrak Kasar Ulva lactuta dalam Meningkatkan Total Haemocyte Count (THC) dan Aktivitas Fagositosis pada Udang Vaname (Litopenaeus vannamei) Suleman Suleman; Sri Andayani; Ating Yuniarti
Samakia : Jurnal Ilmu Perikanan Vol 10 No 1 (2019): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (939.21 KB) | DOI: 10.35316/jsapi.v10i1.230

Abstract

Acauses of the decrease in vaname shrimp production was vibriosis. Vibriosis is caused by vibrio bacteria. A vibrio bacteria that plays a role in vibriosis is vibrio harveyi. Managing of diseases caused by vibrio harveyi is one of the best that provides immunostimulant assistance from natural ingredients derived from seaweed. Ulva lactuta is a green seaweed that has different substances that contain immune. Some of the compositions contained in Ulva Lactutawere Flavonoid Compositions, and the Composition of Polysaccharides that are Combined with Immunimulants The purpose of this study was to determine the effect and dosage used to improve the immune system in vaname shrimp. Combined with THC and Fagocytosis in the hemolim on vaname shrimp before infection and after infection with Vibrio harveyi bacteria.A dose of 2 ppm showed a significant difference (p <0.05) for all treatments.
The Effect of Ulva lactuta Polysaccharide Extract on Total Haemocyte Count and Phagocytic Activity of L. vannamei Suleman Suleman; Sri Andayani; Ating Yuniarti
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.393 KB) | DOI: 10.21776/ub.rjls.2018.005.03.3

Abstract

This research aimed to evaluate the effect of polysaccharide extract of Ulva lactuta on the total haemocytes count (THC) and the phagocytic activities (PA) on Litopenaeus vannamei. The concentration of the extract used in this research was 5 ppm and it was applied through the immersion. The haemolymph was collected after the administration in the different periods at hour of 4 and 24. The results showed that the administration of polysaccharide extracts increased THC and PA in L. vannamei significantly (p<0.05). THC and PA of white-leg shrimp with polysaccharide extract was higher than that without the administration of the extract. The enhancement of THC was found after the administration at hour 4 from 5.00 cells. ml-1 to 5.26 x 104 cells. ml-1. However, the THC level decreased after 24 hours at the value of 5.19 x 104 cells. The increasing pattern was also true for PA of L. vannamei, from 40.44% to 53.67% (hour 4). At the hour of 24, the PA was found decreased to the level and then decreased 45.67 ±1.53. On the contrary, there were no enhancement of THC and PA in the treatment without polysaccharide extract of Ulva lactuta.
Co-Authors Ating Yuniarti Sri Andayani Sri Andayani