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All Journal STOMATOGNATIC- Jurnal Kedokteran Gigi UNEJ e-Proceeding Jurnal Kimia Terapan Indonesia Media Farmasi Indonesia
Dian Agung Pangaribowo
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Arcangelisia flava LEAVES ETHANOLIC EXTRACT SUPPRESSES CANCER CELL LINES VIA NON APOPTOTIC PATHWAY Endah Puspitasari; Dian Agung Pangaribowo; Yora Utami; Ika Yanuar Isparnaning
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Arcangelisia flava is a potential candidate to bedeveloped as cancer chemoprevention agent. A.flava was proven to exhibit antioxidant and cytotoxicactivity against MCF-7 breast cancer cell line. Theseability were suggested to be related to its alkaloidcontent: berberine, palmatine, and jatrorrhizine(Keawpradub et al., 2005). Although this plant isstated as a rarely found species (Koran Jakarta,2012), we could find it abudantly in Meru BetiriNational Park, Jember.Our previous studies showed that A. flava leavesincrease the immune system in doxorubicin-treatedrats (Puspitasari et al., 2014b) with no signs oftoxicity based on sub chronic toxicity assay(Puspitasari et al., 2014a). The A. flava leavesethanolic extract (EEAfL) had been proven to havecytotoxic activity on HeLa, MCF-7, and WiDr cancercell lines with the IC50 value of 467 + 70; 136 + 17;and 213 + 79 μg/ml, respectively. The activity wasselective on MCF-7 and WiDr, but not likely on HeLacell line (Puspitasari et al., 2015).OBJECTIVESThis study was conducted to determine whether thecytotoxic activity of EEAfL was occur via apoptotic ornecrotic pathway using flowcytometry annexin VFITCmethod. The concentration used for the assaywere concentration approx. the IC50 and the IC75based on previous study (Puspitasari et al., 2015).
SINTESIS DAN AKTIVITAS ANTIOKSIDAN 3-(3,4-dimetoksifenil)-1-(4-metilfenil)-2-propen-1-on Dian Agung Pangaribowo; Indah Purnama Sary; Dwi Koko Pratoko
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 11 No 2 (2014)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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A new compound from chalcone derivate has been synthesized from 4-methylacetophenone and 3,4-dimethoxybenzaldehyde by Claisen-Schmidt condensation with catalyst of NaOH 60%. This compound is 3-(3,4-dimethoxyphenyl)-1-(4-methylphenyl)-2-propen-1-one. Structureelucidation was conducted by FTIR and 1H-NMR analysis. The compound is relatively pure and this can be identified by only one spot on TLC analysis and the range of melting point is narrow. Antioxidant activity evaluation of the compound with DPPH method showed that IC50 is 455.312 ppm, lower than vitamin C as the standard.
SINTESIS, UJI AKTIVITAS SITOTOKSIK IN VITRO DAN MOLECULAR DOCKING SENYAWA 1-(4-KLOROBENZOIL)-1,3-DIMETILUREA Dian Agung Pangaribowo; Siswandono Siswandono; Bambang Tri Purwanto
Jurnal Kimia Terapan Indonesia Vol 16, No 1 (2014)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1134.674 KB) | DOI: 10.14203/jkti.v16i1.6

Abstract

Senyawa 1-(4-klorobenzoil)-1,3-dimetilurea telah dirancang, disintesis, diidentifikasi struktur, dan diuji aktivitas sitotoksik secara in vitro. Simulasi docking dilakukan dengan memposisikan senyawa ke dalam sisi aktif reseptor Checkpoint kinase 1 (Chk1) untuk menentukan model pengikatan ligan reseptor. Sintesis 1-(4-klorobenzoil)-1,3-dimetilurea dilakukan lewat reaksi asilasi antara 1,3-dimetilurea dan 4-klorobenzoil klorida. Kemurnian produk hasil sintesis ditentukan dengan metode Kromatografi Lapis Tipis (KLT).Identifikasi struktur dilakukan dengan spektrofotometer UV, FT-IR dan spektrometer NMR. Hasil uji antiproliferatif menunjukkan bahwa senyawa 1-(4-klorobenzoil)-1,3-dimetilurea memiliki aktivitas sitotoksik terhadap sel HeLa yang lebih baik dibandingkan dengan kontrol positif yaitu hidroksiurea. Senyawa 1-(4-klorobenzoil)-1,3-dimetilurea dengan potensi aktivitas sitotoksik ini dapat menjadi agen antikanker yang potensial. Kata kunci: 1-(4-klorobenzoil)-1,3-dimetilurea, molecular docking, sintesis, aktivitas sitotoksik, hidroksiurea A novel 1-(4-chlorobenzoyl)-1,3-dimethylurea has been designed, synthesized, structurally determined, and the in vitro cytotoxic activity was evaluated. Docking simulation was performed to position this compound into the Checkpoint kinase 1 (Chk1) active site to determine the probable binding model. Synthesis of 1-(4-chlorobenzoyl)-1,3-dimethylurea was completed by acylation reaction between 1,3-dimethylurea and 4-chlorobenzoyl chloride. The purity of synthesized product was determined by Thin Layer Chromatography. Structure identification was performed by UV spectrophotometer, FT-IR and NMR spectrometer. Antiproliferative assay result demonstrated that this compound possessed good cytotoxic activity against HeLa cells, which is comparable to the positive control, hydroxyurea. This compound with potent cytotoxic activity might be a potential anticancer agent. Keywords: 1-(4-chlorobenzoyl)-1,3-dimethylurea, molecular docking, synthesis, cytotoxic activity
MOLECULAR DOCKING, SINTESIS DAN UJI AKTIVITAS SITOTOKSIK SENYAWA 1-(3-KLOROBENZOIL)-1,3-DIMETILUREA Dian Agung Pangaribowo
Media Farmasi Indonesia Vol. 8 No. 2 (2013): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

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Abstract

A novel 1-(3-chlorobenzoyl)-1,3-dimethylurea has been designed, synthesized, structurally determined, and the in vitro cytotoxic activity was evaluated. Docking simulation was performed to the position of this compound into the Checkpoint kinase 1 (Chk1) active site to determine the probable binding model. Synthesis of 1-(3-chlorobenzoyl)-1,3-dimethylurea was completed by acylation reaction between 1,3-dimethylurea and 3-chlorobenzoyl chloride. The purity of synthesized product was determined by Thin Layer Chromatography and melting point measurement. Structure identification was performed by UV and FTIR spectrophotometer, NMR spectrometer. Antiproliferative assay result demonstrated that this compound possessed good cytotoxic activity against HeLa cells, which is comparable to the positive control. This compound with potent cytotoxic activity might be a potential anticancer agent.
Co-Authors Bambang Tri Purwanto Dwi Koko Pratoko Endah Puspitasari Ika Yanuar Isparnaning Indah Purnama Sary Siswandono, Siswandono Yora Utami