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BAMBANG MARWOTO
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. ROFIQ SUNARYANTO; BAMBANG MARWOTO; TUN TEDJA IRAWADI; ZAINAL ALIM MAS'UD; LIESBETINI HARTOTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6996.369 KB) | DOI: 10.5454/mi.4.2.7

Abstract

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out. Marine sediment samples were obtained from six different places at Banten West Coast. Isolation was conducted using two pretreatment methods, acid and heat shock pre-treatment. A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134. Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp. Production of active compound from A11 isolate used yeast peptone medium. Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC. A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil YUDHIE ISTIANTO; RADEN SETYO ADJI KOESOEMOWIDODO; YOSHIO WATANABE; HARDANING PRANAMUDA; BAMBANG MARWOTO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.249 KB) | DOI: 10.5454/mi.6.1.7

Abstract

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.
Co-Authors Hardaning Pranamuda Liesbetini Hartoto RADEN SETYO ADJI KOESOEMOWIDODO Rofiq Sunaryanto Tun Tedja Irawadi YOSHIO WATANABE YUDHIE ISTIANTO Zainal Alim Mas'ud