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All Journal Pharmaciana: Jurnal Kefarmasian Majalah Obat Tradisional JURNAL FARMASI GALENIKA Jurnal Riset Kefarmasian Indonesia
Ana Mardiyaningsih
D3 Diploma of Pharmacy, Poltekkes Bhakti Setya
Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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AKTIVITAS SITOTOKSIK EKSTRAK ETANOLIK DAUN ALPUKAT (Persea americana Mill.) PADA SEL KANKER LEHER RAHIM HeLa Mardiyaningsih, Ana; Ismiyati, Nur
Majalah Obat Tradisional Vol 19, No 1 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (697.394 KB) | DOI: 10.14499/mot-TradMedJ19iss1pp%p

Abstract

Kanker leher Rahim merupakan salah satu kanker yang paling terjadi pada wanita. Angka kematian yang tinggi menunjukkan bahwa kemoterapi tidak memberikan hasil yang baik sehingga perlu dikembangkan strategi untuk mengatasinya. Daun alpukat (Persea americana Mill) adalah salah satu produk alami yang memiliki aktivitas antioksidan dengan menangkap radikal bebas dan memiliki aktivitas sitotoksik. Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas sitotoksik dari ekstrak etanolik daun alpukat pada sel kanker leher Rahim HeLa dan mengidentifikasi senyawa aktif  yang ada pada daun alpukat. Senyawa aktif dari daun alpukat diekstraksi dengan metode maserasi menggunakan etanol 96%. Uji kromatografi dilakukan untuk mengidentifikasi senyawa aktif. Aktivitas sitotosik ekstrak etanolik daun alpukat dilakukan dengan uji MTT. Hasil skrining fitokimia menunjukkan bahwa daun alpukat mengandung senyawa golongan flavonoid, alkaloid dan saponin. Ekstrak etanol daun alpukat menghambat pertumbuhan sel HeLa dengan nilai IC50 sebesar360 µg/ml. 
PENGEMBANGAN POTENSI EKSTRAK DAUN PANDAN (Pandanus amaryllifolius Roxb) SEBAGAI AGEN ANTIBAKTERI Mardiyaningsih, Ana; Aini, Resmi
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.377 KB) | DOI: 10.12928/pharmaciana.v4i2.1577

Abstract

Pandanus amayllifolius Roxb which commonly used as a flavoring and food coloring waspotentially have antibacterial activity. It should be an effort to develop a safe naturalpreservatives to reduce cases of food poisoning (foodborne disease) which mainly caused bybacterial pathogens. This study aims to determine the antibacterial potency of the water extract,ethanol, ethyl acetate, and a mixture of ethanol-ethyl acetate (1:1 v/v) extract againstStaphylococcus aureus and Escherichia coli based on the value of MIC (Minimum InhibitoryConcentration) and MBC (Minimum Batericidal Activity). The antibacterial activity wasevaluated by the Kirby-Bauer diffusion method by making the extract concentration of 25 and50 %, as well as the loading dose tested was 2.5 mg and 5 mg/disc. MIC and MBC wasevaluated by solid dilution method. The results showed that the ethanol extract and water extractdon’t have antibacterial activity against Staphylococcus aureus and Escherichia coli, whereasthe ethyl acetate extract and a mixture of ethanol-ethyl acetate (1:1 v/v) have an antibacterialactivity. Ethyl acetate extract showed the highest inhibitory potency. The MIC and MBC was 186 Pharmaҫiana, Vol. 4, No. 2, 2014: 185-192obtained at a level of 1.1% w/v and 6.7% w/v against Staphylococcus aureus, and 0.5% w/v and4.5% w/v against Escherichia coli.
UJI TOKSISITAS SUBKRONIS FRAKSI KLOROFORM DAUN PANDAN (Pandanus amaryllifolius ROXB) SEBAGAI AGEN KOKEMOTERAPI DOKSORUBISIN TERHADAP FUNGSI JANTUNG Ismiyati, Nur; Mardiyaningsih, Ana; Trilestari, Trilestari; Mursyidi, Achmad; Yuwono, Tedjo
JURNAL FARMASI GALENIKA Vol 4 No Edisi Khus (2017): Jurnal Farmasi Galenika Volume 4 Edisi Khusus SemNas Tanaman Obat Indon
Publisher : Sekolah Tinggi Farmasi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.611 KB)

Abstract

Fraksi kloroform daun pandan (Pandanus amaryllifolius) diketahui memiliki efek sinergis sebagai kokemoterapi doksorubisin pada sel kanker payudara MCF-7 dan T47D. Penelitian ini untuk mengetahui efek toksik subkrosis fraksi kloroform daun pandan dikombinasi dengan doksorubisin. Penelitian dilakukan dengan menggunakan tikus Sprague Dawly 1-2 bulan sebanyak 40 ekor dibagi dalam 8 kelompok yang terdiri dari kelompok sehat, kelompok pelarut, kelompok doksorubisin, dan empat kelompok perlakuan kombinasi doksorubisin dan fraksi kloroform. Setelah 30 hari dilakukan pemeriksaan biokimiawi darah  trigliseride, LDL, HDL dan kolesterol. Hasil penelitian menunjukkan kombinasi doksorubisin dengan fraksi kloroform daun pandan tidak menyebabkan toksisitas terhadap jantung dan mampu menurunkan efek toksik doksorubisin terhadap jantung.
PERBEDAAN KUALITAS TANAMAN MINT (Mentha spicata L) HIDROPONIK DAN KONVENSIONAL BERDASARKAN MORFOLOGI TANAMAN, PROFIL KROMATOGRAM, DAN KADAR MINYAK ATSIRI Pratiwi, Pramita Yuli; Mardiyaningsih, Ana; Widarti, Emi
Jurnal Riset Kefarmasian Indonesia Vol 1 No 2 (2019): Jurnal Riset Kefarmasian Indonesia
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.9 KB) | DOI: 10.33759/jrki.v1i2.18

Abstract

Mint is one of the essential oil-producing plants. Essential oils of Mentha spicata L or spearmint are widely used as raw materials in the food, beverage and pharmaceutical preparations industries. The main components of spearmint are karvon, limonen, cineol, dihydrocarvol, myrcene, and 0.5% menthol. The components contained in spearmint have properties as herbal remedies. Plant cultivation greatly determined the results of standardized drug ingredients. Cultivation with the right planting system can affect the quality of simplicia. This research were a descriptive study to differentiate the quality of plants. This study aimed to determine differences in plant morphology, chromatogram profile and levels of spearmint essential oil between hydroponic and conventional planting systems. Observation of plant morphology includes the size, color of the stem and leaves. Essential oil content is calculated from refined oil by steam and water distillation method. Spearmint oil was analyzed on thin layer chromatography methode using the silica gel F254 as a stationary phase and hexane-ethyl acetate (8: 2) as a mobile phase, with spot visualization under UV254 and anisaldehyde-H2SO4 . The results showed that the morphology of Mentha spicata L with hydrophonic planting systems had a bigger size in stem and leaves, but the leaves had more bright green than a conventional plant. The chromatogram profile did not show differences in the number of spots and Rf. Mentha spicata L with conventional planting systems had a sharper intensity of color on one of the volatile oil spot, and suggested due to menthol. The levels volatile oil of Mentha spicata L hydroponic and conventional were determined as 0.0326% and 0.0323% . The Independent T-test analysis showed that there were not a significant differences.
Co-Authors Achmad Mursyidi Nur Ismiyati Pratiwi, Pramita Yuli Resmi Aini Tedjo Yuwono Trilestari, Trilestari Widarti, Emi