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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Molekul: Jurnal Ilmiah Kimia
Asrul Sahri Siregar
jenderal soedirman university
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Veterinary

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EFFECTS OF PHOTOPERIODS ON THE CLONED GTH GENES IN HARD-LIPPED BARB (Osteochillus hasseltii) Norman Arie Prayogo; Taufik Budi Pramono; Asrul Sahri Siregar; Purnama Sukardi; Masashi Kawaichi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 3 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.3.1100

Abstract

Photoperiod is one factor that regulates the endocrine gland’s production of hormones necessary for gonadal growth and development, gametogenesis and reproductive cycles in fish reproduction. However, studies on the influence of photoperiod on tropical fishes are still limited. Hence, this study investigates the effects of photoperiod on the hard-lipped barb, particularly, the genetic expression of its cloned gonadotropin hormones I (GtH-I) and II (GtH-II) genes. Experiment was designed using three treatments; 14L: 10D (control), 8L: 16D (short photoperiods), and 18L: 6D (long photoperiods). Four aquariums with nine fishes/tank were used, serving as replicates. The fishes were kept under these different photoperiod lengths for eight weeks.  Pituitary activities were observed by measuring the GtH genetic expression.  The length of cDNA GtH-I was 222 bp, and the cDNA GtH-II was 354 bp. The GtH-I precursors encoded by cDNA consisted of 67 amino acids, including mature peptide. The level of GtH gene expression significantly increased as longer photoperiods were administered. The results indicated that photoperiod lengths affect the hard-lipped barb reproductive performance.
The Effect of Endosulfan in GnRH and GtH Genes Expression of Male Hard-Lipped Barb (Osteochilus vittatus C.V.) asrul sahri siregar; pudji astuti; yulia sistina; norman arie prayogo
Molekul Vol 15, No 3 (2020)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.261 KB) | DOI: 10.20884/1.jm.2020.15.3.612

Abstract

The presence of pollutants in water makes the environment toxic to all living biota and affects reproduction in fish. One of such pollutants is endosulfan, which is an off-patent organochlorine insecticide, widely used in various agricultural activities. Endosulfan is extremely toxic to fish and all other aquatic organisms, showing a range of chronic effects, including genotoxicity, and reproductive issues. Therefore, the aim of the research was to find the effect of endosulfan on cGnRH, sGnRH, GtH-I and GtH-II gene expression inmale hard-lipped barb fish. These fish were kept in aquarium containing several levels of endosulfan (0 mg/L [control]; 0.88 mg/L [low level]; 1.76 mg/L [medium level]; and 2.64 mg/L [high] level) for 60 days. The effects of endosulfan on GnRH performance on the fishwere evaluated by the expressions of these genes; cGnRH-II, sGnRH, GtH-I, and GtH-II. In the 8th week, there was a decrease in the expression of all the four genes in fish with high dose of endosulfan compared with the control group (P < 0.05). These findings showedthat endosulfan inhibits the expression of cGnRH, sGnRH, GtH-I and GtH-II genes in male hard-lipped barb.
Co-Authors Masashi Kawaichi norman arie prayogo Norman Arie Prayogo pudji astuti Purnama Sukardi Taufik Budi Pramono Yulia Sistina