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Martinius Martinius
Department of Plant Protection, Faculty of Agriculture, Andalas University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education

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Karakterisasi Genetik TRICHODERMA SPP Indigenus Rizosfir Pisang yang Berpotensi Pengendalian FUSARIUM OXYSPORUM F. SP. CUBENSE Penyebab Penyakit Layu Fusarium pada Pisang Nurbailis, Nurbailis; Martinius, Martinius
Sainstek Vol 11, No 1 (2008): Vol. XI No. 1, September 2008
Publisher : Lembaga Penelitian Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.116 KB)

Abstract

The objective of the research was to characterize the genetic variation of Trichoderma spp. Study of genetic characters used Random Amplified Polymorphic DNA-Polymerase Chain-reaction (RAPD-PCR) that amplicated with four primers (OPA 2, OPA 17, OPB- 05 and Primer 2).  Analysis result of genetic variation of Trichoderma spp make as dendrogram. The result of the research indicated that : 1) Trichoderma spp. had the great genetic variation, 2)Trichoderma isolate from the same region did not always have the same genetic profile, 3) S6 and T1 isolates were effective to inhibit the growth of Fusarium oxysporum f. Sp. cubense  than the others but  had a different genetic profile. Keywords : Trichoderma spp, Fusarium oxysporum f.sp.cubense, karakterisasi genetik
Pengaruh Kolonisasi Trichoderma spp. pada Akar Bibit Pisang terhadap Perkembangan Penyakit Layu Fusarium (Fusarium oxysporum f. sp. cubense) Nurbailis, Nurbailis; Martinius, Martinius
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.5 KB) | DOI: 10.31258/jnat.13.3.220-225

Abstract

The purpose of the research was to obtain the superior Trichoderma that had ability to colonize root with the resultbeing effective to supress Fusarium wilt desease and promote banana seedling growth. This experiment consistedof 2 factors and 4 replications. The first factor was Trichoderma spp. namely : A. Trichoderma koningii strain S6sh(TK-S6sh), B Trichoderma viride strain T1sk (TV-T1sk) and Trichoderma harzianum strain S10sh (TH-S10sh). Thesecond factor was the kind of banana namely a.Cavendis, b. Barangan and c. Kepok. The observation werecolonization ability, Fusarium wilt desease development and the banana seedling growth. The result showed thatTV-T1sk was the best spesies to colonize all banana seedling root. The highest colonization in Barangan bananaseedling root reached 80%. Trichoderma colonization in banana seedling root could suppress Fusarium wilt diseasedevelopment and increase banana seedling weight. Higher ability of Trichoderma to colonize banana seedling rootcaused lower disease incidence of Fusarium wilt and greater biomass of banana seedling. Interaction betweenTV-T1Sk and Barangan banana was the best in colonization, so they were effective to suppress Fusarium wiltdesease and increase banana seedling biomass.
Co-Authors Eka Candra Lina Nurbailis Supriadi, Adventus Yunisman, Yunisman