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Karakterisasi Genetik TRICHODERMA SPP Indigenus Rizosfir Pisang yang Berpotensi Pengendalian FUSARIUM OXYSPORUM F. SP. CUBENSE Penyebab Penyakit Layu Fusarium pada Pisang Nurbailis, Nurbailis; Martinius, Martinius
Sainstek Vol 11, No 1 (2008): Vol. XI No. 1, September 2008
Publisher : Lembaga Penelitian Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.116 KB)

Abstract

The objective of the research was to characterize the genetic variation of Trichoderma spp. Study of genetic characters used Random Amplified Polymorphic DNA-Polymerase Chain-reaction (RAPD-PCR) that amplicated with four primers (OPA 2, OPA 17, OPB- 05 and Primer 2).  Analysis result of genetic variation of Trichoderma spp make as dendrogram. The result of the research indicated that : 1) Trichoderma spp. had the great genetic variation, 2)Trichoderma isolate from the same region did not always have the same genetic profile, 3) S6 and T1 isolates were effective to inhibit the growth of Fusarium oxysporum f. Sp. cubense  than the others but  had a different genetic profile. Keywords : Trichoderma spp, Fusarium oxysporum f.sp.cubense, karakterisasi genetik
Pengaruh Kolonisasi Trichoderma spp. pada Akar Bibit Pisang terhadap Perkembangan Penyakit Layu Fusarium (Fusarium oxysporum f. sp. cubense) Nurbailis, Nurbailis; Martinius, Martinius
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.5 KB) | DOI: 10.31258/jnat.13.3.220-225

Abstract

The purpose of the research was to obtain the superior Trichoderma that had ability to colonize root with the resultbeing effective to supress Fusarium wilt desease and promote banana seedling growth. This experiment consistedof 2 factors and 4 replications. The first factor was Trichoderma spp. namely : A. Trichoderma koningii strain S6sh(TK-S6sh), B Trichoderma viride strain T1sk (TV-T1sk) and Trichoderma harzianum strain S10sh (TH-S10sh). Thesecond factor was the kind of banana namely a.Cavendis, b. Barangan and c. Kepok. The observation werecolonization ability, Fusarium wilt desease development and the banana seedling growth. The result showed thatTV-T1sk was the best spesies to colonize all banana seedling root. The highest colonization in Barangan bananaseedling root reached 80%. Trichoderma colonization in banana seedling root could suppress Fusarium wilt diseasedevelopment and increase banana seedling weight. Higher ability of Trichoderma to colonize banana seedling rootcaused lower disease incidence of Fusarium wilt and greater biomass of banana seedling. Interaction betweenTV-T1Sk and Barangan banana was the best in colonization, so they were effective to suppress Fusarium wiltdesease and increase banana seedling biomass.
VIRULENSI BERBAGAI ISOLAT JAMUR ENTOMOPATOGEN METARHIZIUM SPP. TERHADAP HAMA PENGGEREK BUAH KAKAO CONOPOMORPHA CRAMERELLA SNELL. (LEPIDOPTERA: GRACILLARIIDAE) . Trizelia; . Nurbailis; Dina Ernawati
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.742 KB) | DOI: 10.23960/j.hptt.213151-158

Abstract

Virulence of various Metarhizium spp. (Deuteromycotina: Hyphomycetes) isolates on cocoa pod borrer pest, Conopomorpha cramerella Snell. (Lepidoptera: Gracillariidae). In Indonesia, Conopomorpha cramerella Snell. (Lepidoptera : Gracillariidae) is a serious pest of cocoa. Entomopathogens such as Metarhizium spp. (Deuteromycotina: Hyphomycetes) can be used to control this pest as an alternative to chemical insecticides. The purpose of this research was to study the virulence of isolates of Metarhizium spp. to pupae of C. cramerella. Nine isolates of Metarhizium spp each with 107 conidia/ml rate were used in the experiment. Pupal mortality was recorded daily. The results showed that isolate MetApCi from chili rhizosphere had the highest virulence causing 96.67% mortality of C. cramerella pupae and with an LT50 of 3.04 days.
KESINTASAN BEBERAPA JAMUR ANTAGONIS PADA BUAH CABAI DAN POTENSINYA DALAM MENEKAN PENYAKIT ANTRAKNOSA YANG DISEBABKAN OLEH COLLETOTRICHUM GLOEOSPORIOIDES Naipinta, Rizka; ., Martinius; ., Nurbailis
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.069 KB) | DOI: 10.23960/j.hptt.217162-169

Abstract

Persistence of several antagonistic fungus on chilli and its potential to suppress anthracnose disease caused by Colletotrichum gloeosporioides. Anthracnose disease caused by C. gloeosporioides is one of the important diseases on chilli because its directly gives negative impact on chilli production. The aim of this research was to obtain the superior antagonistic fungi that have ability to persist on chili fruit and potential to control anthracnose disease caused by C. gloeosporioides  The experiment consist of two units: 1. Testing of persistence ability of antagonistic fungi on chilli fruit, 2. Testing the potential of antagonistic fungi to suppress anthracnose disease on chilli fruit. Both of the test used Randomized Block Design (RBD) with 10 treatments and 4 replication, each of replication consist of 4 chilies fruit. Those treatments were Trichoderma-PP1, Trichoderma-PP3, Trichoderma-AG2, Trichoderma-PYK3, Paecilomyces-PP6, Paecilomyces-PP7, Paecilomyces-AG4, Paecilomyces-PYK4, Aspergillus PP2 and without antagonistic fungi (control). The result indicated that all antagonistic fungi isolate could persist on chili fruit. The highest persistence were Trichoderma-PP3 and TrichodermaAG2 (95.83%) and the lowest belonged to Paecilomyces-PP7 (50%). Trichoderma-PP3 and Trichoderma-AG2 were the best isolates for suppressing anthracnose disease caused by C. gloeosporioides.
Keanekaragaman Jamur pada Rizosfer Tanaman Cabai Sistem Konvensional dan Organik dan Potensinya sebagai Agen Pengendali Hayati Colletotrichum gloeosporioides Nurbailis .; Martinius .; Verry Azniza
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.828 KB) | DOI: 10.23960/j.hptt.11416-24

Abstract

ABSTRACTFungal diversity of chili rhizosphere at conventional and organic cropping system and its role as biocontrol agent of Colletotrichum gloeosporioides. The aim of this research was to study fungal diversity in chili rhizosphere at conventional and organic system; to ditermine and identify the fungi that had antagonistic activity against C. gloeosporioides. Diversities of rhizosphere fungi in chili of conventional and organic system were determined by counting the amount of propagule and isolates. Antagonistic activity was examined by dual cultures and slide culture. Identification was determind up to genus level. Propagule density and the amount of isolates in chili rhizosphere of organic system were higher than those in conventional system. Fifty two fungal isolates were found from chili rhizosphere at conventional and organic system, 28 isolates from organic system and 24 isolates from conventional. Ten fungal isolates from chili rhizosphere in organic system and 4 fungal isolates from that in conventional system had antagonistic activity against C. gloeosporioides which cause antracnose on chili. The antagonistic isolates were: Trichoderma, Paecilomyces, Aspergillus and unidentified isolate (X isolate).
PENGGUNAAN TRICHODERMA SPP UNTUK PENGENDALIAN PENYAKIT DAN PENINGKATAN PERTUMBUHAN TANAMAN CABAI DI NAGARI TAEH BARUAH KECAMATAN PAYAKUMBUH KABUPATEN LIMAPULUH KOTA Yulmira Yanti; Nurbailis Nurbailis; Hasmiandy Hamid; Trizelia Trizelia; Haliatur Rahma; My Syahrawati; Noveriza Hermeria
Jurnal Hilirisasi IPTEKS Vol 4 No 1 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i1.491

Abstract

Jorong Parit Dalam Nagari Taeh Baruah, Payakumbuh District, Limapuluh Kota Regency is one of the villages that has various problems such as drought, high number of idle lands and remote access. The main crop planted during the rainy season is lowland rice, but most of the land becomes idle land during the dry season. In terms of fertilization, farmers in this location still use artificial fertilizers (chemical fertilizers) and in terms of pest and disease control they still use chemical pesticides. The solutions that can be offered are planting chili plants which are more resistant to drought, processing agricultural waste into compost as an alternative to reduce dependence on the use of chemical fertilizers, use of Trichoderma and vegetable pesticides as agents for controlling pests and diseases, and processing chili production to increase value. selling, competitiveness and community income. The purpose of this farmer group empowerment activity is to increase public knowledge about the use of Trichoderma which can increase the growth of chili plants, and can also be used as a biological agent to control pests and diseases that often attack chili plants when cultivated. The method used in this activity is socialization and counseling, structured training and direct practice in the field of Trichoderma's influence on the growth and production of chili plants. The results obtained from this activity are that the community can recognize that there are alternative controls that are more environmentally friendly than the use of synthetic pesticides. The public can also gain new understanding regarding the increased growth and yield of chili plants by utilizing the Trichoderma biological agent. So that in the end it can reduce dependence on synthetic pesticides obtained from outside. Therefore, the environment and the balance of the ecosystem in the Nagari Taeh Baruh area are maintained. The conclusion of this activity is that plant growth applied using Trichoderma spp is higher than plants that are not treated with Trichoderma spp (control). The use of Trichoderma spp is highly recommended because besides being easy to obtain, it also provides positive values for plant growth and is good for environmental technology around cultivated plants.
Eksplorasi Jamur-Jamur yang Berasosiasi dengan Penyakit Mati Meranting pada Tanaman Kakao di Kota Padang Nurbailis Nurbailis; Rummah Yulisyah; Novri Nelly
JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Vol 5 No 1 (2021): Juni 2021
Publisher : Jurusan Hama dan Penyakit Tumbuhan Faperta Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.1.1-11.2021

Abstract

Branch dieback is one of the primary diseases in cacao plants. The disease affects young shoots and twigs, causes leaves to die, and leaves them bare, interfering with photosynthesis. This study aimed to determine the causative pathogens in Padang City. The locations were: Sungai Sarik Kuranji, Lubuk Minturun Koto Tangah, and Teluk Kabung Bungus. The locations have the following criteria: age ? three years, the number of plants at the location ? 50 individuals, and found symptoms of branch dieback. The research used descriptive methods. The sample represented 10% of the total number of plants at the location, was collected randomly. The results found four species of fungi associated with the disease, namely Oncobasidium theobromae, Botrydiplodia theobromae, Fusarium decemcellulare, and Fusarium sp1.
PEMANFAATAN AGEN HAYATI UNTUK PENGELOLAAN OPT CABAI PADA KELOMPOK TANI SIMABUR SUKSES MAKMUR DI KECAMATAN PARIANGAN KABUPATEN TANAH DATAR Trizelia Trizelia; Nurbailis Nurbailis; Yulmira Yanti; Winarto Winarto; Haliatur Rahma; Martinius Martinius; Yenny Liswarni; Rusdi Rusli; Yunisman Yunisman; Darnetty Darnetty; Eri Sulyanti
Jurnal Hilirisasi IPTEKS Vol 2 No 3.b (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (940.813 KB) | DOI: 10.25077/jhi.v2i3.b.331

Abstract

Cabai merah merupakan sumber pendapatan utama bagi petani di daerah Tanah Datar. Masalah utama yang selalu dihadapi oleh petani cabai di daerah ini adalah serangan hama dan penyakit yang dapat menyebabkan kehilangan hasil yang cukup tinggi. Hingga saat ini, pengendalian hama dan penyakit sayuran masih mengandalkan pestisida sintetik yang telah menimbulkan dampak negatif. Untuk itu, perlu dicari alternatif pengendalian yang dapat mengurangi dampak negatif pestisida tersebut, yaitu menggunakan agens hayati seperti cendawan Beauveria bassiana dan Trichoderma sp.Tujuan dari kegiatan pengabdian kepada masyarakat ini adalah untuk memperkenalkan dan memasyarakatkan pengendalian hama dan penyakit menggunakan agens hayati Beauveria bassiana dan Trichoderma sp. Metode kegiatan pengabdian adalah penyuluhan, pelatihan dan praktek lapangan. Minat petani untuk menggunakan agens hayati terutama Beauveria bassiana dan Trichoderma cukup tinggi dan telah memotivasi mereka untuk mengurangi penggunaan pestisida sintetis. Petani juga telah berhasil memperbanyak agens hayati dan membuat kompos limbah pertanian menggunakan Trichoderma.
Profil GCMS Senyawa Kimia Ekstrak Metanol Isolat Cendawan Entomopatogen Beauveria Bassiana Dan Akar Cabai Sebagai Pemacu Pertumbuhan Cabai Saragih, Magdalena; Trizelia, Trizelia; Nurbailis, Nurbailis; Yusniwati, Yusniwati
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol 4, No 2 (2020): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, Juni
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.232 KB) | DOI: 10.31289/agr.v4i2.3840

Abstract

The aim of this study was to identify the chemical compound methanol extract of entomopathogenic fungus Beauveria bassiana from insect walang sangit and the chemical compound roots of red chili plants that were able to stimulate the growth of chilli plants after being applied with entomopathogenic fungus   B. bassiana through seed immersion inoculation using GCMS method. The chemical compound identified as a growth booster in   B. bassiana fungus isolates from the insect walang sangit is an Acetic acid Ethanoic acid Ethylic acid Glacial acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methylpalmitate Uniphat A60, n-Hexadecanoic acid Hexadecanoic acid n-Hexadecoic acid Palmitic acid, Dianhydromannitol and Ergosta-5,7,22-trien-3-ol, (3.beta., 22E) - (CAS) Ergosterol (CAS), while the chili root contains Acetic acid Ethanoic acid Ethylic acid Glacials acetic acid CH3COOH, Hexadecanoic acid, methyl ester (CAS) Methyl palmitate Uniphat A60, n -Hexadecanoic acid Hexadecanoic acid Palmitic acid, 8, 11- octadecadienoic acid, methyl ester (CAS) METHYL 8, 11-OCTADECADIENOATE, (23S)-ethylcholest-5-en-3.beta.-ol.s It can be concluded that some of the chemical compounds in methanol extracts identified in chili roots have similarities with chemical compounds that exist in   B. bassiana entomopathogenic fungus which are potential as stimulers of chili plant growth
PENINGKATAN PRODUKSI BAWANG MERAH MELALUI APLIKASI YUYAOST DAN TRICHODERMA DI KELOMPOK TANI NGUNGUN JORONG GANTIANG UTARA Yulmira Yanti; Hasmiandy Hamid; Trimuti Habazar; Reflin Reflin; Nurbailis Nurbailis; Yaherwandi Yaherwandi; Muzilatul Nilisma; Annisya Diadinni
Jurnal Hilirisasi IPTEKS Vol 2 No 4.a (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.872 KB) | DOI: 10.25077/jhi.v2i4.a.303

Abstract

Tanaman bawang merah memiliki potensi pengembangan sangat baik. Permintaan pasar akan komoditi tanaman bawang merah cenderung meningkat berkaitan dengan pertumbuhan jumlah penduduk,. tujuan dari kegiatan pengabdian masyarakat ini ialah meningkatkan produksi tanaman bawang merah ngungun jorong Gantiang Utara dengan cara yang efektif dan efisien melalui pemberian YUYAOST dan Trichoderma. Metode pelaksanaan kegiatan meliputi survei pendahuluan pendekatan sosial, penyuluhan, pelatihan, dan pembuatan demplot. Kegiatan ini sangat bermanfaat bagi masyarakat khususnya kelompok tani ngungun jorong Gantiang Utara. Antusias dan partisipasi aktif dari masyarakat sangat menunjang keberhasilan dari kegiatan ini. Pertumbuhan dan hasil produksi yang terbaik didapatkan pada perlakuan YUYAOST. Dalam penerapan teknologi menggunakan YUYAOST dan Trichoderma sangat membantu percepatan tumbuhan tanaman bawang merah. Penggunaan YUYAOST sangat disarankan karena selain mudah untuk didapatkan juga memberikan nilai positif terhadap pertumbuhan tanaman dan baik untuk teknologi lingkungan sekitar tanaman budidaya.