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Pendampingan Entrepreneurship Budidaya dan Pemasaran Ikan Cupang (Betta sp.) oleh Alumni Jurusan Akuakultur, Universitas Bangka Belitung Kurniawan, Andri
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 4, No 1 (2021): Januari 2021
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33633/ja.v4i1.143

Entrepreneurship assistance to alumnus's business ideas is a practice that cannot be separated from the obligations and devotion of a lecturer as a form of dedication to the independence and existence of alumnus in society. This service activity aimed to provide assistance to alumni of the Aquaculture Department, Bangka Belitung University in pioneering and building a betta fish (Betta sp.) cultivation and marketing business. This activity was carried out through the stages of introducing the potential for ornamental fish cultivation, building relationships with ornamental fish cultivators and marketers, and formulating promotional strategies in starting businesses, marketing, and developing alumni businesses. This mentoring activity resulted in a pilot business for ornamental fish cultivation and marketing, especially for betta fish, namely the Exotic Betta. The business that was initiated within three months has shown business prospects with an increase in sales and expansion of the marketing area, both in Pangkalpinang City and regencies in Bangka Island to areas outside Bangka Island. The promotion and marketing of the Exotic Betta betta fish through online media and social media has made this business unit quickly recognized by betta fish hobbyists. Business development needs to be done by diversifying commodities to increase and develop marketing business for ornamental fish.

Chronosequence Effect of Post Tin Mining Ponds to Metals Residu and Microecosystem Change Andri Kurniawan
Journal Omni-Akuatika Vol 13, No 1 (2017): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

The ecological change caused by tin mining activity is among the most important sorts of problem in the environment, including heavy metals contamination and change of microecosystem. This article aim to identify speciation of metals and heavy metals in post tin mining aquatic ecosystem, particularly, disused tin mining ponds in West Bangka, Indonesia in a chronosequence effect study. Speciation of metals and heavy metals are detected by X-Ray Fluorescence. X-Ray Fluorescence analysis show that heavy metals in post tin mining ponds like Fe, Sn, and Cu and also some metals such as Mg, Si, Al, S, and P were detected with a different numbers. There is a speciation of heavy metals and metals in post tin mining ponds with chronosequence effect. It can become an important and basic study about potential bioremediator in the research locations. Further, researches about microecosystem change and interaction of microorganisms and heavy metals there can become an information to design an ecological management tool to manage this ecosystem so it can be used for primay or secondary activity.

The Phosphorus and Sulphur Distribution and Culturable Bacterial In Time Chronosequence of Ex-Tin Mining Ponds Andri Kurniawan; Oedjijono Oedjijono; Tamad Tamad; Uyi Sulaeman
Journal Omni-Akuatika Vol 14, No 3 (2018): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

The tin mining had caused ecological changes that can be occured to macro- and microecosystem. This article aims to study the pattern distribution of element of phosphorus (P) and sulphur (S) and also to identify culturable bacterial that were isolated form ex-tin mining ponds in time chronosequence. The elemets of P and S were detected by X-Ray Fluorescence (XRF) and the bacterials was isolated in medium agar and biochemistry identification by microbact. The concentration of element of P and S showed the average of P concentration increased in time chronosequnce of ex-tin mining ponds, whereas the average of S concentration showed dynamic pattern. In ex-tin mining pond with age < 1 year the average concentration of P was 33,725 mg.L-1 and S was 311,45 mg.L-1. In ex-tin mining pond with age 5-10 years were P (59,8 mg.L-1) and S (451,75 mg.L-1). In ex-tin mining pond with age > 15 years were P (67,44 mg.L-1) and S (386,125 mg.L-1). While, the culturable bacterials were Kurthia spp; Actinobacillus equuli; Bacillus amyloliquefaciens; Bacillus spp; Micrococcus sp; Enterobacter gergoviae; Veillonella sp; Enterobacter aerogenes; Moraxella bovis; Nitrobater spp; and Enterobacter agglomerans.

Short Communication: Biochemistry Analysis and Molecular Approach to Identify the Cultured Bacterial from Ex-Tin Mining Lakes Andri Kurniawan; Oedjijono Oedjijono; Tamad Tamad; Uyi Sulaeman
Jurnal Ilmu Lingkungan Vol 20, No 3 (2022): July 2022
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.20.3.563-569

There are two methods to identify the bacterial characteristic, namely biochemical analysis and the 16S ribosomal ribonucleic acid gene (16S rRNA) sequencing analysis. The research aimed to identify the cultured bacterial from ex-tin mining lakes by biochemistry analysis and molecular approach. Nine bacterial were cultured and isolated in nutrient agar and then biochemically characterized by microbact™ 12A and 24E (Oxoid) identification kits. In addition, molecular analysis by 16S rRNA gene was sequenced primer 1492R and primer 27F. Based on biochemistry analysis, these bacterial were identified as belonging to species of Bacillus amyloliquefaciens; Enterobacter gergoviae; Enterobacter aerogenes; Enterobacter agglomerans; and Nitrobacter spp. The sequence analysis in gene bank of NCBI indicated that these species had similarity with Klebsiella variicola strain F2R9 (Accession NR_025635.1); Enterobacter cloacae subsp. dissolvens strain LMG 2683 (Accession NR_044978.1); Serratia marcescens strain NBRC 102204 (Accession NR_114043.1); Bacillus marisflavi strain TF-11 (Accession NR_118437.1); Falsibacillus pallidus strain CW 7 (Accession NR_116287.1); Klebsiella pneumoniae strain DSM 30104 (Accession NR_117683.1); and Nitrobacter winogradskyi strain Nb-255 (Accession NR_074324.1). However, phylogenetic tree was constructed by Neighbor-Joining Test showed the cultured bacterial were not in the same clade and also with Salmonella enterica subsp. enterica strain LT2 (Accession NR_074910.1); Bacillus amyloliquefaciens strain BCRC 11601; and Escherichia coli strain NBRC 102203 (Accession NR_114042.1) as in group species and Micrococcus luteus strain NCTC 2665 (Accession NR_075062.2); Chloroflexus islandicus strain isl-2 (Accession NR_148571.2); Flavobacterium gondwanense (Accession M92278.1); and Cytophaga aurantiaca strain JM110 (Accession MN758870.1) as their out group.ABSTRAKTerdapat dua metode untuk mengidentifikasi karakteristik bakteri, yaitu analisis biokimia dan analisis sekuensing gen 16S ribosomal ribonucleic acid (16S rRNA). Karakterisasi bakteri telah dilakukan melalui analisis morfologi dan biokimia dan dikonfirmasimelalui pendekatan molekuler menggunakan sekuensing gen 16S ribosomal ribonucleic acid (16S rRNA). Penelitian ini bertujuan untuk mengidentifikasi bakteri yang dapat dikultur dari danau pascatambang timah melalui analisis biokimiawi dan pendekatan molekuler. Sembilan bakteria berhasil dikultur dan diisolasi di media nutrient agar dan kemudian secara biokimiawi dikarakterisasi menggunakan microbact™ 12A and 24E (Oxoid) identification kits. Lebih lanjut, analisis molekuler menggunakan gen 16S rRNA dilakukan sekuensing dengan primer 1492R dan primer 27F. berdasarkan analisis biokimia, bakteri-bakteri tersebut termasuk ke dalam spesies Bacillus amyloliquefaciens; Enterobacter gergoviae; Enterobacter aerogenes; Enterobacter agglomerans; dan Nitrobacter spp. Analisis blasting pada gene bank di NCBI mengindikasikan bahwa spesies-spesies tersebut memiliki kemiripan atau similaritas dengan Klebsiella variicola strain F2R9 (Accession NR_025635.1); Enterobacter cloacae subsp. dissolvens strain LMG 2683 (Accession NR_044978.1); Serratia marcescens strain NBRC 102204 (Accession NR_114043.1); Bacillus marisflavi strain TF-11 (Accession NR_118437.1); Falsibacillus pallidus strain CW 7 (Accession NR_116287.1); Klebsiella pneumoniae strain DSM 30104 (Accession NR_117683.1); dan Nitrobacter winogradskyi strain Nb-255 (Accession NR_074324.1). Namun, pohon filogenetik yang dikonstruksikan dengan Neighbor-Joining Test menunjukkan bahwa bakteri yang dikultur tersebut tidak berada pada clade dan juga dengan Salmonella enterica subsp. enterica strain LT2 (Accession NR_074910.1); Bacillus amyloliquefaciens strain BCRC 11601; dan Escherichia coli strain NBRC 102203 (Accession NR_114042.1) yang digunakan sebagai spesies in group species maupun Micrococcus luteus strain NCTC 2665 (Accession NR_075062.2); Chloroflexus islandicus strain isl-2 (Accession NR_148571.2); Flavobacterium gondwanense (Accession M92278.1); dan Cytophaga aurantiaca strain JM110 (Accession MN758870.1) sebagai out groupnya.

Review Tentang Kemampuan Ikan Ekstremofil Untuk Hidup Di Perairan Asam Dan Terkontaminasi Logam Berat Pascapenambangan Timah Andri Kurniawan; Diah Mustikasari
Jurnal Ilmu Lingkungan Vol 19, No 3 (2021): November 2021
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.19.3.541-554

Ikan ekstremofil (extremophile fishes) telah muncul sebagai model untuk kajian biologi integratif. Ikan-ikan ini tidak hanya memberikan wawasan tentang proses biologis, biokimia, fisiologis, dan perkembangan kehidupan organisme, tetapi juga penjelasan tentang kapasitas dan keterbatasan hidup untuk beradaptasi dan bertahan hidup dalam kondisi lingkungan ekstrem. Beberapa ikan ekstremofil dapat bertahan hidup di bawah kondisi habitat yang dianggap tidak ramah bagi sebagian besar ikan karena adanya stresor lingkungan. Ikan ekstrofil sering mengembangkan mekanisme adaptasi yang kompleks untuk mengatasi faktor stresor. Salah satu lingkungan yang ekstrim adalah kolong bekas penambangan timah yang terletak di Provinsi Kepulauan Bangka Belitung sebagai penghasil timah. Beberapa ikan yang ditemukan dapat beradaptasi dengan kondisi ekstrem habitat ini; pH rendah, oksigen terlarut rendah, nutrisi rendah, dan kontaminasi logam berat tinggi. Habitat ekstrem yang terjadi secara alami dapat dianggap sebagai penelitian evolusioner yang memungkinkan mempelajari kemampuan ikan untuk beradaptasi dan bertahan hidup terhadap kondisi ekologi yang berubah. Telaah artikel ini bertujuan untuk memberikan gambaran tentang karakteristik perairan kolong bekas penambangan timah dan fisiologi ikan ekstremofil ditinjau dari modifikasi fisikokimia dan biokimianya. Hasil kajian makalah penelitian menunjukkan beberapa jenis ikan seperti Aplocheilus sp., Betta sp., Gambusia sp., Rasbora sp., Belontia sp., Brevibora sp., Oryzias sp., Puntius sp., Anabas sp., dan Trichogaster sp. mampu toleran pada kondisi ekstrem perairan kolong pascapenambangan timah yang terbengkalai. Kemampuan ikan untuk beradaptasi dan bertahan hidup di lingkungan ekstrem seperti perairan kolong pascapenambangan timah didukung oleh kemampuan untuk melakukan penundaan penetasan telur dengan memasuki fase diapause. Kemampuan ikan lainnya adalah pengaturan mekanisme osmoregulasi atau homeostatis tubuh terhadap kondisi pH asam maupun kontaminasi logam berat di lingkungan tersebut.ABSTRACTExtremophile fishes have emerged as veritable models for investigations in integrative biology. These fishes not only provide insights into biological, biochemical, physiological, and developmental processes of organism’ life, but also the explanation of life’s capacity and limitation to adapt and survive in the extreme environmental conditions. Some extremophile fishes can survive under habitat conditions considered inhospitable for most fishes due to the presence of the environment stressors. The extremophile fishes have often evolved complex adaptation mechanisms to cope the stressor factors. One of the extreme environments is abandoned tin mining ponds, located in Bangka Belitung Archipelago Province as a tin producer. Some fishes have found can adapt to the extreme conditions of this habitat; low pH, low dissolved oxygen, low nutrition, and highly heavy metals contamination. Naturally occurring extreme habitat can be regarded as evolutionary researches that allow studying the ability of fishes to adapt and survive to altered ecological conditions. This paper review aimed to provide an overview about water characteristics of abandoned tin mining pits and physiology of extremophile fishes in terms of modification of physicochemical and biochemical. The result of research papers review indicated some species of fish such as Aplocheilus sp., Betta sp., Gambusia sp., Rasbora sp., Belontia sp., Brevibora sp., Oryzias sp., Puntius sp., Anabas sp., and Trichogaster sp. able to tolerate to the extreme conditions of abandoned tin mining pit waters. The ability of fish to adapt and survive in extreme environments such as abandoned tin mining pit waters is supported by the ability to delay hatching of eggs by entering the diapause phase. Another ability is the regulation of the body's osmoregulation or homeostatic mechanism against acidic pH conditions and heavy metal contamination in the environment.

Determinasi nilai pH untuk memprediksi kualitas perairan pada kolong pascatambang timah di Pulau Bangka Euis Asriani; Andri Kurniawan
Journal of Aquatropica Asia Vol 1 No 2 (2015): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar

The study in predicting quality of water in ex-tin mining lakes in Bangka Island aims to identify the condition of the waters through the measurement of various parameters of waters, formulating models of a possible relationship between various parameters of water at the pit measured and formulate the relationship parameters of pH with other water quality parameters. Sampling conducted research sites in each district / city in Bangka Island with cluster sampling. Measurement of water quality parameters include measurements of pH, dissolved oxygen (DO), temperature, turbidity, and the heavy metals polluted level. The relationship of all the results mathematically analyzed using multiple linear regression as a model of post-tin mining water quality. In the old lakes, obtained fairly linear relationship between temperature parameters pH, DO, and brightness with a correlation coefficient of R = 0.81 and coefficient of determination R2 = 65%. In both types of lakes, relationship model between pH with other parameters is not significant in linearity. Based on test results of heavy metals, some metals were detected in water samples was Pb, Cd and Fe.

The Pattern of Heavy Metals Distribution in Time Chronosequence of Ex-Tin Mining Ponds in Bangka Regency, Indonesia Andri Kurniawan; Oedjijono Oedjijono; Tamad Tamad; Uyi Sulaeman
Indonesian Journal of Chemistry Vol 19, No 1 (2019)
Publisher : Universitas Gadjah Mada

The heavy metals distribution of ex-tin mining ponds were investigated. The time chronosequence was determined at the pond of age < 1 year (Station A), the pond of age 5–10 years (Station B), and the pond of age > 15 years (Station C). The results showed sixteen heavy metals of As, Co, Cu, Cr, Fe, Ga, Hf, Sn, Ta, Te, Th, Mn, Ni, Pb, Zn, and V could be detected in the ponds. The metals such as As, Co, Cu, Ga, Mn, Ni, Pb, Th, and Zn in Station C showed higher concentration compared to the Station A and Station B. The metals such as Cr, Fe, Hf, Sn, Ta, Te, and V in Station A and Station B showed higher concentration compared to the Station C. The positive, negative, and dynamic correlation pattern could be found in distribution of heavy metal to time chronosequence. The concentration of Ta and V showed a positive correlation because their concentration decrease, whereas concentration of As, Cu, Ga, Mn, and Zn showed a negative correlation because their concentration increase along in time chronosequence. The dynamic correlation could be found that concentration of Co, Ni, Pb, Sn, and Th decrease from Station A to Station B and then increase in Station C, whereas concentration of Cr, Fe, Hf, and Te increase from Station A to Station B and then decrease in Station C.

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