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Tias Widyastuti
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KONDROGENESIS ADIPOSE-DERIVED STEM CELLS MENGGUNAKAN PLATELET-RICH PLASMA PADA SCAFFOLD SUTRA Imam Rosadi; Karina Karina; Komang A. Wahyuningsih; Iis Rosliana; Tias Widyastuti; Siti Sobariah; Irsyah Afini; Anggraini Barlian
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2666.248 KB) | DOI: 10.15408/kauniyah.v13i1.12053

Abstract

AbstrakStudi tentang kemampuan adipose-derived stem cells (ADSCs) sebagai sel punca yang dapat berdiferensiasi menjadi kondrosit menggunakan platelet-rich plasma (PRP) sebagai subtitusi fetal bovine serum (FBS) telah banyak dilaporkan. Penggunaan medium pertumbuhan dengan kombinasi ADSCs, PRP dan scaffold sutra masih belum banyak dipelajari dalam rekayasa jaringan kartilago. Studi ini bertujuan untuk mempelajari pengaruh medium yang mengandung 5%, 10% dan 20% PRP terhadap proses kondrogenesis ADSCs manusia yang dikultur pada scaffold sutra Bombyx mori Indonesia. Metode penelitian diawali dengan pembuatan scaffold sutra menggunakan metode salt-leaching, isolasi dan kultur ADSCs manusia dari jaringan lemak, uji pertumbuhan ADSCs pada scaffold sutra dengan variasi konsentrasi PRP pada medium serta analisis kadar glikosaminoglikan (GAG). Hasil penelitian menunjukkan bahwa ADSCs yang dikultur menggunakan PRP lebih tinggi laju pertumbuhannya dibandingkan dikultur menggunakan FBS selama 7 hari pengamatan. Kadar GAG yang disekresikan ADSCs kelompok PRP juga lebih tinggi dibandingkan kelompok FBS. Kadar GAG tertinggi pada hari ke-21 pengamatan adalah medium yang mengandung 20% PRP kemudian 10% dan 5%, sedangkan kadar GAG kelompok kontrol cenderung stabil pada kadar yang rendah. Berdasarkan hasil tersebut, medium yang mengandung PRP memiliki potensi dalam menginduksi kondrogenesis ADSCs yang dikultur pada scaffold sutra.Abstract The studies on adipose-derived stem cells (ADSCs) differentiation into chondrocytes using platelet-rich plasma (PRP) as a substitute for fetal bovine serum (FBS) have been reported. However, the combination of ADSCs, PRP and silk fibroin scaffold has not been widely studied for developing cartilage engineering. Therefore, this research aims to study the effect of medium containing 5%, 10% and 20% PRP towards chondrogenesis of human ADSCs cultured on silk fibroin scaffold from Indonesia Bombyx mori. At first, the silk fibroin scaffold was fabricated using a salt-leaching method, then ADSCs were isolated and cultured from adipose tissues. The assays of growth curve and biocompatibility of silk fibroin scaffold toward ADSCs supplemented by PRP as well as glycosaminoglycans (GAG) concentration were conducted later. The results showed that higher absorbance of proliferation rate was on ADSCs supplemented by various PRP concentrations compare to FBS control group for seven days of observation. Level of GAG, which secreted by ADSCs supplemented by a various concentration of PRP, was also higher than the FBS group. The highest level of GAG on day 21 was observed in 20% PRP group then 10% and 5% PRP, while a group of GAG level is stable at low levels. This study concludes that PRP has the potential to induce chondrogenesis ADSCs which cultured on silk fibroin scaffold.
EXPRESSION OF PROTEIN CD73/CD90/CD105/CD34/CD45/CD11b/CD19/HLA-DR ON STEM CELLS FROM HUMAN FAT TISSUE, USING CYTOMETRY FLOW Imam Rosadi; Karina Karina; Iis Rosliana; Siti Sobariah; Irsyah Afini; Tias Widyastuti; Anggraini Barlian
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.707 KB) | DOI: 10.15408/kauniyah.v12i2.8751

Abstract

AbstrakSel punca merupakan sel yang dapat membelah dan berdiferensiasi menjadi sel jenis lainnya. Sel punca asal jaringan lemak potensial dikembangkan sebagai salah satu alternatif sel punca yang bersumber dari limbah sedot lemak manusia. Sel punca asal jaringan lemak akan mengekspresikan protein spesifik penanda permukaan CD73, CD90, CD105 dalam persentase yang tinggi dan CD34/CD45/CD11b/CD19/HLA-DR dalam persentase yang rendah. Studi ini bertujuan untuk memanfaatkan limbah sedot lemak manusia dengan melakukan isolasi sel punca asal jaringan lemak dan menguji protein penanda permukaan spesifik sel punca. Beberapa tahapan dalam studi ini adalah isolasi stromal vascular fraction (SVF) dan kultur sel punca asal jaringan lemak manusia, population doubling time (PDT) serta analisis protein penanda permukaan CD7Ee3, CD90, CD105, dan CD34/CD45/CD11b/CD19/HLA-DR pada pasase ke-1 dari 3 donor. Hasil dari studi ini menunjukkan bahwa sel dari jaringan lemak berhasil dikultur dengan durasi pembelahan sel adalah 3,3 hari. Sel mengekspresikan CD73 (99,79%), CD90 (94,17%), CD105 (48,75%), dan CD34/CD45/CD11b/CD19/HLA-DR (kurang dari 2%). Ekspresi CD105 yang rendah dari ketiga donor diduga berkaitan dengan tingkatan pasase sel yang digunakan. Berdasarkan hasil tersebut dapat disimpulkan bahwa sel punca asal jaringan lemak pasase ke-1 telah mengekspresikan ketiga marker protein penanda permukaan sel punca, yaitu CD73, CD90 dan CD105.Abstract Stem cells are cells that can divide into other different types of similar cells. Stem cells from fat tissue potential have been developed as an alternative stem cell from human liposuction. Stem cells from fat tissue will express high protein-specific markers on CD73, CD90, CD105 and CD34/CD45/CD11b/CD19/HLA-DR in a low percentage. This study aims to utilize human liposuction waste by isolating stem cells from fat tissue and testing protein-specific stem cell surface markers. Some stages in this study are isolation of stromal vascular fraction (SVF) and stem cell culture from human fat tissue, population doubling time (PDT) and protein analysis of surface markers CD73, CD90, CD105, and CD34/CD45/CD11b/CD19/HLA-DR on the 1st passage of 3 donors. The results of this study showed that cells from fat tissue were successfully cultured with cell division duration of 3.3 days. Cells expressed CD73 (99.79%), CD90 (94.17%), CD105 (48.75%), and CD34/CD45/CD11b/CD19/HLA-DR (less than 2%). The low expression of CD105 from all three donors is thought to be related to the level of cell passage used. Based on these results, it can be concluded that the stem cells from first passage fat tissue have expressed the three protein markers of stem cell surface markers, namely CD73, CD90 and CD105.
Co-Authors Anggraini Barlian Anggraini Barlian Iis Rosliana Imam Rosadi Irsyah Afini Karina Karina Komang A. Wahyuningsih Siti Sobariah