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Lies Setijaningsih
Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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KINERJA PERBEDAAN SALINITAS TERHADAP RESPON PERTUMBUHAN DAN GAMBARAN DARAH BENIH IKAN TAMBAKAN (Helostoma temminckii) Lies Setijaningsih; Imam Taufik; Deni Radona; Mulyasari Mulyasari
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3003

Abstract

Water salinity is a crucial factor in Kissing Gourami (Helostoma temminckii) culture. Therefore, information regarding suitable and optimum medium for the ecophysiological requirement of fish is needed. Salinity is one physiological factor that affects both the survival and growth rate of fish as it is related to the osmoregulation process. The purpose of this study was to analyze the effect of different water salinity on, growth, survival rate, osmotic activity and blood picture of Kissing Gourami seed nursery. Fish was cultured in various salinity levels: 0 ppt (A), 1 ppt (B), 3 ppt (C), and 5 ppt (D). Salinity level significantly affected the survival rate, absolute weight growth, absolute length growth, specific growth rate, and blood picture of Kissing Gourami seed nursery. It was found that the Kissing Gourami seeds had the optimum growth on the salinity of 3 ppt, that was further shown by 82,00% of survival rate, 2,42% ±2,81 g/day of specific growth rate obtained. The results showed a positive correlation of the biological value of fish with the content of osmotic gradient, blood glucose, hematocrit, hemoglobin, erythrocytes amount, and water quality parameters (dissolved oxygen, temperature, pH, and ammonia). 
PERFORMA PEMIJAHAN IKAN BELIDA (Notopterus chitala) MELALUI INDUKSI HORMON HCG DAN LHRH Lies Setijaningsih; Jojo Subagja; Deni Radona; Brata Pantjara; Anang Hari Kristanto; Yohanna Retnaning Widyastuti
Jurnal Riset Akuakultur Vol 13, No 2 (2018): (Juni, 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.251 KB) | DOI: 10.15578/jra.13.2.2018.115-122

Abstract

Permintaan ikan belida cukup tinggi untuk memenuhi kebutuhan konsumsi dan bahan baku olahan makanan yang masih mengandalkan tangkapan dari alam. Tingginya eksploitasi mengakibatkan populasi ikan dari tahun ke tahun semakin berkurang dan terancam punah. Teknologi pembenihan ikan belida dengan penggunaan hormon diduga dapat meningkatkan produksi larva untuk keperluan budidaya. Penelitian dilakukan untuk mengevaluasi performasi pemijahan ikan belida secara alami dan buatan melalui induksi hormon HCG dan LHRH analog. Pemijahan alami dan buatan dilakukan menggunakan induk betina sebanyak enam ekor dan jantan tiga ekor (rasio 2:1). Induk yang digunakan berukuran panjang 46 ± 5 cm dan bobot 2.209 ± 623 g. Pemijahan buatan dilakukan dengan induksi hormon HCG dosis 500 IU/kg dan LHRH analog dosis 0,5 mL/kg. Penyuntikan HCG dan LHRH diberikan dengan interval 24 jam. Hasil penelitian menunjukkan pada pemijahan buatan (induksi hormon) diperoleh induk yang memijah sebanyak dua ekor dengan nilai diameter telur (3,1 ± 0,3 mm); fekunditas (282-907 butir); derajat pembuahan (21%-40%); derajat penetasan (56%-75%); dan sintasan (30%-50%); sedangkan pada pemijahan alami diperoleh satu ekor induk yang memijah dengan nilai diameter telur (3,5 ± 0,3 mm); fekunditas (1.616 butir); dan derajat pembuahan (86,7%); selama tiga bulan pengamatan secara intensif dengan frekuensi pemijahan enam kali. Aplikasi teknologi pemijahan dengan menggunakan induksi hormon dapat menghasilkan produk larva pada ikan belida.The demand for fish consumption of Clown Knifefish is considerably high. However, the fish supply for consumption and processed food still relies on wild-caught. Such exploitation has decreased the population of Clown Knifefish to a point that it can become an endangered species. Efforts on the breeding technology of Clown Knifefish through natural and artificial propagation using of hormones have shown promising results. The current study was conducted to evaluate the performance of Clown Knifefish in natural and artificial spawning conditions using the induction of HCG hormone and LHRH analogues. The natural and artificial spawning treatments were conducted using six fish females and three fish males (a ratio of 2:1). The average Broodstocks’ size was 46 ± 5 cm in length and 2,209 ± 623 g in weight. The artificial spawning was conducted with HCG hormone induction (dose of 500 IU/kg body weight) and LHRH analogue (dose: 0.5 mL/kg). HCG and LHRH injections were given within 24 hours interval. The results showed that the artificial spawning (hormone induction) had successfully caused two broodstock to spawn producing eggs with the diameter of 3.1 ± 0.3 mm; fecundity of 282-907 eggs; fertilization rate of 21%-40%; hatching rate of 56%-75%; and survival rate of 30%-50%. In the natural spawning, one broodstock had spawned with the egg diameter of 3.5 ± 0.3 mm; fecundity of 1,616 eggs; and fertilization rate of 86.7%. This study concludes that spawning technology applications using hormonal induction can increase the production larvae of Clown Knifefish broodstock.
Co-Authors Anang Hari Kristanto Brata Pantjara Deni Radona Imam Taufik Jojo Subagja Mulyasari Mulyasari Yohanna Retnaning Widyastuti