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All Journal Jurnal Kimia Riset
Dwi Putri Mashfufatur Rohmah
Departemen Kimia, Fakultas Sains dan Teknologi Universitas Airlangga, Surabaya
Chemistry

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PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya Dwi Putri Mashfufatur Rohmah; Sofijan Hadi; Afaf Baktir
Jurnal Kimia Riset Vol. 4 No. 2 (2019): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v4i2.16902

Abstract

AbstractThis research has done partial purification by fractionation and optimization crystallization of papain from Carica papaya latex with the addition of ammonium sulphate. The enhancement of purification factor was determined by its specific activity. The fractionation results show that papain fraction of Carica papaya can be obtained by adding 40-80% saturated ammonium sulphate, with the highest specific activity, i.e. 2,0819 U/µg and the factor purification increase of 6,27 fold than papain extract. Meanwhile, the highest total activity, i.e. 10,7780 U of papain crystals can be obtained by presipitant agent addition of ammonium sulphate in the level / concentration 80% saturated at 15 °C. Microscopycally papain crystals profile in this condition have cube and tetragonal shape.Key words: crystallization, fractionation, ammonium sulphate, papain
Co-Authors AFAF BAKTIR Sofijan Hadi