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All Journal Folia Medica Indonesiana Majalah Obstetri dan Ginekologi
Kuntaman Kuntaman
Department of Medical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr Soetomo Hospital, Surabaya
Health Professions Medicine & Pharmacology Other

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Pattern of disease and type of operation of Surgical Site Infection in obstetrics and gynecology at Dr Soetomo Hospital, Surabaya, Indonesia Ardi Eko Marsanto; Kuntaman Kuntaman; Hari Paraton; Budi Prasetyo
Majalah Obstetri dan Ginekologi Vol. 27 No. 2 (2019): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.374 KB) | DOI: 10.20473/mog.V27I22019.49-55

Abstract

Objectives: to find correlation between risk factors and classification of SSI, the distribution of SSI, and cost in Soetomo Hospital.Materials and Methods: descriptive retrospective observational with correlation analysis between risk factors and SSI classification according to Centers for Disease Control (CDC) definition with univariate analysis. Sub-group descriptive analysis on microbiology result, length of stay (LOS) and cost was also performed. All patients was diagnosed SSI between January 2015 until June 2017.Results: age, referral cases, Body Mass Index, Hemoglobin and Albumin results, types of surgery, types of case are the risk factors we included. Analysis result shows no strong and significance correlation in all risk factors (r 0.053 – 0.447; p 0.072 – 0.971). According to SSI types, the distribution are: superficial (7; 13.2%), deep (26; 49.1%), organ/space (20; 37.7%). Extended-Spectrum Beta-Lactamase (ESBL) was obtained in 19 (14 Obstetrics; 5 Gynecology) from 28 patients with microbiology results. Five deaths occured in ESBL patients (1 Obstetrics; 4 Gynecology; ratio 1 : 11.2). Mean length of stay in Obstetrics and Gynecology was 41.7 and 19.2 days, respectively. Mean cost per day in Obstetrics and Gynecology was 1.2 amd 2.6 million rupiah, respectively.Conclusions: no strong and significant results in all risk factors. Death rate in SSI and malignancy with positive ESBL result are high. Less cost needed for SSI patients, but with longer LOS. Comprehensive approaches are needed to patients at risk of SSI, further study with larger sample are needed.
The Correlation between icaA and icaD Genes with Biofilm Formation Staphylococcus epidermidis In Vitro Dian Rachmawati; Kuntaman Kuntaman; Lindawati Alimsardjono
Folia Medica Indonesiana Vol. 55 No. 4 (2019): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.506 KB) | DOI: 10.20473/fmi.v55i4.24388

Abstract

This study was conducted to identify the presence of icaA and icaD genes in S. epidermidis and to analyze the relationship between the presence of icaA and icaD genes with the ability of in vitro biofilm formation in S. epidermidis. S. epidermidis isolates from patients and healthy people were collected and PCR was examined to detect icaA and icaD genes. which then continued to examine the ability of biofilm formation by the method of Congo Red Agar. The results of this genotypic and phenotypic examination were then tested for correlation with statistical tests using SPSS 23.0. A total of 40 S. epidermidis isolates were collected, consisting of 20 clinical isolates and 20 isolates of normal flora. The icaA gene was positive in 5 isolates (12.5%), and 8 isolates (20%) were positive for the icaD gene, 3 isolates with icaA and icaD were both positive. One hundred percent of isolates with icaA or icaD positively formed biofilms, but there were 15 isolates (42.9%) who did not have the icaA gene but showed the ability to form biofilms, while 12 isolates (37.5%) who did not have the icaD gene also formed biofilms. Fifty percent of S. epidermidis isolates showed the ability to form biofilms at CRA. The Fisher Exact test showed a significant relationship between the icaA gene and the ability of biofilm formation (p=0.047 (p<0.05)) as well as the icaD gene (p=0.03 (p<0.05)). The icaA and icaD genes have a significant relationship to biofilm formation in S. epidermidis. There was another mechanism in the formation of biofilms that are not dependent on the ica gene.
Co-Authors Alimsardjono, Lindawati Ardi Eko Marsanto Budi Prasetyo Dian Rachmawati Hari Paraton