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Zona hambat ekstrak etil asetat daun kemangi (Ocimum basilicum) terhadap Enterococcus faecalis ATCC 29212Inhibition zone of ethyl acetate extract of basil leaves (Ocimum basilicum) against Enterococcus faecalis ATCC 29212 Rahmi Wastri; Ame Suciati Setiawan; Dani Rizali Firman; Diani Prisinda; Fajar Fatriadi
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 1 (2021): April 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i1.28836

Pendahuluan: Enterococcus faecalis (E. faecalis) merupakan bakteri yang persisten pada saluran akar gigi, sehingga dapat menyebabkan infeksi sekunder pada saluran akar gigi yang telah diobturasi. Bahan irigasi dan medikamen harus diberikan pada saluran akar gigi untuk menunjang keberhasilan perawatannya. Daun kemangi (Ocimum basilicum/O. basilicum) merupakan tanaman obat tradisional yang diketahui memiliki potensi antibakteri dan berpeluang untuk dikembangkan sebagai bahan irigasi saluran akar gigi. Penelitian ini bertujuan untuk mengetahui zona hambat ekstrak etil asetat daun O. basilicum terhadap E. faecalis ATTC 29212. Metode:Jenis penelitian eksperimental laboratori. Pengujian zona hambat ekstrak etil asetat daun O. basilicum yang diperoleh dari proses maserasi dilakukan dengan metode difusi cakram dengan menggunakan klorheksidin 0,1% sebagai kontrol positif dan dilakukan tiga kali pengulangan terhadap E. faecalis ATCC 29212. Ekstrak pekat dilarutkan menggunakan DMSO 10% dan dilakukan pengenceran dua tingkat sehingga diperoleh konsentrasi ekstrak 512.000 – 1.000 ppm. Hasil: Ekstrak etil asetat daun O. basilicum pada konsentrasi uji 512.000 ppm telah memperlihatkan zona abu-abu seluas 1 mm di luar cakram uji, sementara konsentrasi uji lainnya tidak memperlihatkan adanya penghambatan. Beragam faktor dalam penelitian ini dapat mempengaruhi hasil pengujian, diantaranya adalah kelarutan ekstrak uji dalam pelarut yang digunakan, prosedur sebelum ekstraksi, dan keterbatasan metoda pengujian. Simpulan: Ekstrak etil asetat daun O. basilicum tidak memiliki zona hambat terhadap E. faecalis ATTC 29212.Kata kunci: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zona hambat. ABSTRACTIntroduction: Enterococcus faecalis (E. faecalis) is a bacteria that persist in root canals, cause secondary infection in obturated root canals. Irrigation material and medicament must be administered to the root canal to support the treatment success. Basil leaves (Ocimum basilicum/O. basilicum) is a traditional medicinal plant known with antibacterial potential and has the opportunity to be developed as a root canal irrigation agent. This study was aimed to determine the inhibition zone of the ethyl acetate extract of O. basilicum leaves against E. faecalis ATTC 29212. Methods: This study was an experimental laboratory. The inhibition zone test of the ethyl acetate extract of O. basilicum leaves obtained from the maceration process was conducted with the disc diffusion method using 0.1% chlorhexidine as a positive control and three replications E. faecalis ATCC 29212. The concentrated extract was dissolved using 10% DMSO, and two dilution levels were carried out to obtain an extract concentration of 512,000 - 1,000 ppm. Results: The ethyl acetate extract of O. basilicum leaves at the test concentration of 512,000 ppm showed a grey zone of 1 mm outside the test disc, while other test concentrations did not show any inhibition. Various factors in this study can affect the test results, including the solubility of the test extract in the solvent used, the procedure before extraction, and the limitations of the test method. Conclusion: O. basilicum leaf ethyl acetate extract had no inhibition zone against E. faecalis ATTC 29212.Keywords: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zone of inhibition.

Perbedaan pH saliva sesudah konsumsi kismis Thompson seedless raisinDifferences in the salivary pH after consumption of Thompson seedless raisin Ayu Vidya Putri; Sri Tjahajawati; Ame Suciati Setiawan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 30, No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Pendahuluan: Kismis Thompson seedless raisin (Vitis vinifera sultanina) mengandung fruktosa dan glukosa yang dapat meningkatkan rangsang manis. Rangsang manis akan meningkatkan sekresi saliva yang berpengaruh pada pH saliva. Tujuan penelitian ini untuk mengetahui perbedaan pH saliva sebelum dan sesudah konsumsi kismis Thompson seedless raisin. Metode: Jenis penelitian eksperimental semu dengan analisis statistik uji Wilcoxon. Jumlah sampel 38 mahasiswa Kedokteran Gigi Universitas Padjadjaran berusia 18-24 tahun. Pengukuran pH saliva dilakukan sebelum dan sesudah mengonsumsi kismis Thompson seedless raisin. Hasil: Nilai rata-rata pH saliva sebelum mengonsumsi kismis Thompson seedless raisin adalah 6,87 dan sesudah mengonsumsi kismis Thompson seedless raisin adalah 7,20. Analisis statistik menunjukan peningkatan pH saliva yang bermakna (p-value < 0,05) antara sebelum dan sesudah mengonsumsi kismis Thompson seedless raisin. Simpulan: Terdapat peningkatan nilai pH saliva sebelum dan sesudah konsumsi kismis Thompson seedless raisin.Kata kunci: Kismis Thompson seedless raisin, pH saliva. ABSTRACT Introduction: Thompson seedless raisin (Vitis vinifera sultanina) contains fructose and glucose which can increase sweet stimulation bud. Sweet stimulation will increase salivary secretion which affects the salivary pH. The purpose of this study was to determine differences in the salivary pH before and after consumption of Thompson seedless raisin. Methods: This research type was quasi-experimental research with Wilcoxon test statistical analysis. The number of samples was as much as 38 dentistry students from Universitas Padjadjaran aged 18 – 24-years-old. Measurement of the salivary pH was performed before and after consuming Thompson seedless raisin. Result: The average value of salivary pH before consuming Thompson seedless raisin was 6.87 and after consuming Thompson seedless raisin was 7.20. Statistical analysis showed a significant increase in the salivary pH (p-value < 0.05) before and after consuming Thompson seedless raisin. Conclusion: There was a significant increase in the salivary pH value before and after consumption of Thompson seedless raisin.Keywords : Thompson seedless raisins, salivary pH.

The inhibitory effectiveness of ethanol extract of selasih leaves towards Streptococcus sanguis growth Ivhatry Rizky Octavia Putri Susilo; Mieke Hemiawati Satari; Ame Suciati Setiawan
Padjadjaran Journal of Dentistry Vol 27, No 1 (2015): March 2015
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol27no1.26694

Introduction: Selasih is one of tropical and subtropical plant used as traditional medicine. It has antibacterial activity especially toward Gram-positive bacteria because it contains volatile oils, tannin, flavonoid and tertepenoid. Method: The study was performed as an experimental laboratory study used Kirby Bauer agar diffusion applied to eight samples of Streptococcus sanguis which was done by twice repetition for each sample. The concentrations of ethanol extract were 16%, 8%, 4%, 2% and 1%.The result was analysed using ANOVA method for single factor of experimental design. The result of the study showed that there was evidence of the antibacterial effect contained in the ethanol extract of selasih leaves inhibiting the growth of Streptococcus sanguis . The average of inhibitory zone for each concentration in every sample, were: 5,25 mm for 16%, 3,22 mm for 8%, 1,84 mm for 4%, 1,53 mm for 2% and 0,19 mm for 1%. The conclusion of the study indicates that there is an antibacterial effects in ethanol extract of selasih leaves (Ocimum basilicum Linn) which inhibits the growth of Streptococcus sanguis. This antibacterial strength is caused by the active content of selasih leaves extract.Conclusion: The extract of basilicum ethanol (Ocimum basilicum L) effectively inhibits the growth of Streptococcus sanguis.

Antibacterial effect of cinnamon essential oil (Cinnamon cassia) in different concentration towards Streptococcus sanguis Hanizul Hajanajumudin; Mieke Hemiawati Satari; Ame Suciati Setiawan
Padjadjaran Journal of Dentistry Vol 22, No 2 (2010): July 2010
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no2.26845

Introduction: Streptococcus sanguis is a type of normal bacteria found in the oral cavity, particularly in plaque on tooth surfaces and can also be found in saliva, buccal mucosa, tongue and gingival sulcus. At present, the usage of herbal remedy for reducing plaque in oral cavity has been implemented widely and one of the herbal remedy used is cinnamon essential oil. The purpose of this study is to prove that there are differences in the antibacterial activity of cinnamon essential oil with different concentrations against Streptococcus sanguis. Methods: The study was performed using laboratory experiments, in which the sensitivity of the Streptococcus sanguis bacteria from the saliva of 10 KPBI batch 2006 dental students were tested in vitro against cinnamon essential oil by using Kirby-Bauer method. Results: The study shows that the cinnamon essential oil has different antibacterial abilities in inhibiting the growth of Streptococcus sanguis in different concentrations. This is due to the element cinnamaldehyde and eugenol that they posses as their main constituent. There is a significant difference between the antibacterial effects of Cinnamon cassia essential oil in different concentrations towards Streptococcus sanguis. Conclusion: Cinnamaldehyde and eugenol has been proved to have antibacterial effect towards wide variety of bacteria including Streptococcus sanguis.

The difference of inhibitory zone between Katuk (Sauropus androgynous L. Merr.) leaf infusion and Roselle (Hibiscus sabdariffa L.) petals towards oral Candida albicans Kadek Seruni Kusumanegara; Emma Rachmawati; Ame Suciati Setiawan
Padjadjaran Journal of Dentistry Vol 29, No 2 (2017): July 2017
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Katuk (Sauropus androgynus L. Merr.) leaf and roselle (Hibiscus sabdariffa L.) are part of plants that have antifungal activity against Candida albicans. The purpose of this study was to examine the inhibitory zone of katuk leaf’s infusion compared to roselle petals towards the growth of oral Candida albicans. Methods: The research methods was experimental laboratory. Each of katuk leaf and roselle’s infusion were respectively made in four concentration, i.e 5, 10, 20, and 40%. The positive control was 2% ketoconazole, while negative control was sterile aquadest. Results: The result of the study showed that the mean of inhibitory zones of katuk leaf 5%, was 17.90±0.8 mm, katuk leaf of 10% was 19.67±1.78 mm, katuk leaf of 20% was 19.67±1.78 mm, and katuk leaf consentration of 40% was 22.93±1.00 mm, meanwhile the mean of inhibitions zones of roselle petals infusion of 5% shows its mean of 18.53±0.67 mm, roselle petals of 10% was 22.40±1.28 mm, roselle petals of 20% was 26.20±0.87 mm, and roselle petals of 40%was 29.47±2.87 mm. Conclusion: It can be concluded that the mean of inhibitions zones of katuk leaf’s infusion was smaller than roselle petals toward the growth of oral Candida albicans.

Correlation of premature loss and molar relationship of mixed dentition children with a very high def-t index Hanna Widyawati; Yuliawati Zenab; Ame Suciati Setiawan
Padjadjaran Journal of Dentistry Vol 33, No 2 (2021): July 2021
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no2.23621

Introduction: Premature loss mainly occurs in mixed dentition periods in their maxillary deciduous and/or mandibular canine, first molar, and/or second molar. The mixed dentition stage occurs in elementary school-aged children, mark with malocclusions and tooth decays, which can be observed from a very high def-t index. This condition will also lead to an abnormal molar relationship. This study was aimed to analyse the correlation of premature loss and molar relationship of mixed dentition children with a very high def-t index. Methods: An analytical observational study with a cross-sectional approach was conducted towards 43 elementary children of Ciawi, Cikeruh 1, and Cikeruh 2 Public Elementary School (SDN), Jatinangor Regency, West Java, Indonesia, aged 7-9 years. The sample was chosen using the purposive sampling method, based on the sample criteria and their willingness to be research subjects. The premature loss of deciduous teeth and primary first molar relationship were observed through extraoral and intraoral examination. The correlation of the premature loss and molar relationship was analysed using Spearman’s rank correlation test. Results: Premature loss significantly affected the permanent first molars relationship in children with no succedaneous teeth (p=0.02, p<0.05), however, it was showed no significant effect in children with succedaneous teeth (p=0.07, p>0.05). The Spearman’s rank correlation test results showed a positive correlation between premature loss and molar relationship of mixed dentition children with a very high def-t index. Conclusion: Premature loss significantly affected the permanent first molars relationship in children with no succedaneous teeth. However, it was showed no significant effect in children with succedaneous teeth. A positive correlation is found between premature loss and molar relationship of mixed dentition children with a very high def-t index. The higher the premature loss, the more abnormal molar relationship will be found in mixed dentition children with a very high def-t index.

Exposure time of virgin coconut oil against oral Candida albicans Lui Dwen Tjin; Ame Suciati Setiawan; Emma Rachmawati
Padjadjaran Journal of Dentistry Vol 28, No 2 (2016): July 2016
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Virgin Coconut Oil (VCO) is an oil which is purely made as a product of the coconut palmand has been used for centuries in the culinary industry and the medical field. Currently, VCO is used widely in medical field due to its effectiveness as antibacterial, antiviral and antifungal properties. Objective of this experimental study was to prove the effectiveness of virgin coconut oil by determining its exposure time at concentration of 25% to inhibit growth of oral Candida albicans. Methode: The research was done by quasi experiment design using the serial dilution method. The exposure time test was performed using a spread plate technique with virgin coconut oil at concentration of 25 % against oral Candida albicans isolated from saliva. Intervals of day 0, 1, 2 and 3 were used. Result: shows that virgin coconut oil inhibited the growth of oral C. albicans cells at day 2. Conclusion: virgin coconut oil with concentration of 25% can inhibit the growth of oral C. albicans with the exposure time of 2 days, which is similar with other antifungals.

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