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All Journal Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Natur Indonesia
Chairul Muluk
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences

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Aktivitas Promoter â-aktin Ikan Medaka Jepang (Oryzias latipes) pada Ikan Mas (Cyprinus carpio) ', Alimuddin; Purwanti, Lola Irma; Ath-thar, MH. Fariduddin; Muluk, Chairul; Carman, Odang; Sumantadinata, Komar
Jurnal Natur Indonesia Vol 11, No 2 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.918 KB) | DOI: 10.31258/jnat.11.2.70-77

Abstract

This study was conducted to examine activity of medaka (Oryzias latipes) â-actin promoter (mBP) in common carp(Cyprinus carpio) as the first step towards development of common carp transgenic in country. Gene constructpmBP-hrGFP that consists of mBA promoter and humanized Renilla reniformis green fluorescent protein gene(hrGFP) was injected into cytoplasm of one cell stage of common carp by using microinjector. PmBP-hrGFPconcentration used for microinjection was 50 μg/mL aquabides. Parameters observed were survival rate of embryo(SRe), hatching rate (HR) and expression of hrGFP gene. SRe was calculated before eggs hacthed, while hatchingrate (HR) was after all of eggs hatched. The activity of mBA promoter was analyzed by observation of hrGFP genetransient expression using a fluorescence microscope. The results of experiment showed that SRe (87,5%) andHR (79.2%) of control was respectevily higher than that of injected treatment (75.0% & 61.7%). Expression of hrGFPwas observed firstly at blastula (12 hours after fertilization) to 1-day-old larval stages (24 hours after hatching)with higher gene expression at blastula to late gastrula stages. Percentage of micronjected larvae expressinghrGFP at 6 hours after hatching reached 71.6 ± 6.7%. Conclusion was that mBA promoter could drove hrGFPexpression in common carp, hence it can be used to produce common carp transgenic by changing hrGFP withgenes correlated with important traits in aquaculture.
TINGKAT PEMANFAATAN PAKAN DAN KELAYAKAN KUALITAS AIR SERTA ESTIMASI PERTUMBUHAN DAN PRODUKSI UDANG VANAME (Litopenaeus vannamei, Boone 1931) PADA SISTEM INTENSIF Tatag Budiardi; Chairul Muluk; Bambang Widigdo; Kardiyo Praptokardiyo; Dedi Soedharma
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 15 No. 2 (2008): Desember 2008
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.405 KB)

Abstract

ABSTRAKPenelitian tingkat pemanfaatan pakan dan kelayakan kualitas air serta estimasi pertumbuhan dan produksi udang vaname (Litopenaeus vannamei) pada sistem intensif telah dilakukan di Pelabuan Ratu, Jawa Barat pada bulan Mei sampai Agustus. Tujuan penelitian ini adalah untuk mengevaluasi tingkat pemanfaatan pakan dan kelayakan kualitas air pada sistem budidaya udang vaname intensif. Penelitian ini didasarkan pada observasi enam tambak selama satu masa pemeliharaan (100 hari) dengan desain kausal dan metode ex postfactountuk mendapatkan data kualitas air dan produksi udang. Hasil penelitian menunjukkan, bahwa penurunan kualitas air mulai terjadi pada pemeliharaan hari ke-40 dan terus menurun sampai akhir pemeliharaan. Tingkat pemanfaatan pakan yang tinggi menghasilkan kelayakan kualitas air dan laju pertumbuhan yang tinggi sehingga menghasilkan produksi biomassa udang yang tinggi. Model hubungan jumlah pakan yang diberikan (x) dan biomassa yang dihasilkan (y) berupa regresi kuadratik y = 0.00006x2 + 1.3506x + 7.3864 (R2 = 0.9801) sehingga biomassa maksimum tercapai pada 7 593 kg dengan pemberian pakan sebanyak 11 255 kg atau FCR sebesar 1.48.Kata kunci: sistem intensif, udang, pakan, kualitas air, biomassa, tambak.ABSTRACTA study on feed utilization, and the suitability of water quality and the estimation of vaname shrimp (Litopenaeus vannamei) growth and production of an intensive system was conducted in Pelabuan Ratu, West Java during Mei-Agustus. The research was aim at evaluating feeding practices, and suitability of its water quality obtained. This study was based on observations six ponds during one growout period (100 days) with causal design and ex post-facto method to obtain data on water quality and production. The result showed that degradation of water quality occurred not until the 40th day of cultivation, and progressively decreased up to the end of the growout period. The high level of feed utilization produced suitable water quality, and high shrimp growth rates, thus, yielding high shrimp biomass. Feed-shrimp biomass relationship could be expressed by the following quadratic regression: y = 0.00006x2 + 1.3506x + 7.3864 (R2 = 0.9801), from which the maximum shrimp biomass was reached at 7 593 kg on 11 255 kg feed, giving a feed conversion ratio of 1.48.Keywords: intensive system, shrimp, feed, water quality, biomass, tambak.
Co-Authors ', Alimuddin Dedi Soedharma Kardiyo Praptokardiyo Komar Sumantadinata Lola Irma Purwanti MH. Fariduddin Ath-thar Odang Carman Tatag Budiardi