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Novarina Sulsia Ista'In Ningtyas
Universitas Pendidikan Mandalika
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Resistensi Escherichia coli Terhadap Berbagai Macam Antibiotik pada Pasien Kucing Rumah Sakit Hewan Pendidikan Universitas Pendidikan Mandalika Alfiana Laili Dwi Agustin; Novarina Sulsia Ista'In Ningtyas
Media Kedokteran Hewan Vol. 33 No. 2 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i2.2022.63-71

Abstract

Kurang efektifnya antibiotik yang digunakan dalam terapi pada pasien yang terinfeksi bakteri salah satu indikasi adanya resistensi terhadap antibiotik. Resistensi antibiotik merupakan permasalahan yang berkaitan dengan kesehatan manusia, hewan dan lingkungan. Hewan beserta lingkungan dianggap sebagai salah satu reservoir munculnya bakteri resisten yang dapat berpindah ke manusia baik secara langsung maupun tidak langsung, dapat pula melalui makan dan lingkungan. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik bakteri Escherichia coliterhadap antibiotik Penicillin G, Tetracyline, dan Cefotaxime. Pengujian dilakukan pada 12 sampel kucing yang satang untuk diperiksa di Rumah Sakit Hewan Pendidikan, Universitas Pendidikan Mandalika. Hasil pegujian menujukan dari 12 sampel tersebut menunjukan bahwa bakteri Escherichia coli yang resisten terhadap penicillin G sebesar 91,7%, resisten terhadap tetracycline 58,3% dan resisten terhadap antibiotik cefotaxime sebesar 33,3%.
Resistensi Antibiotik terhadap Bakteri Escherichia coli yang Diisolasi dari Ayam Layer di Desa Sesaot Kabupaten Lombok Barat Alfiana Laili Dwi Agustin; Novarina Sulsia Ista'In Ningtyas; Kunti Tirtasari
Media Kedokteran Hewan Vol. 33 No. 2 (2022): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v33i2.2022.87-95

Abstract

Resistensi antibiotik saat ini menjadi ancaman terbesar bagi kesehatan masyarakat global, sehingga WHO mengkoordinasi kampanye global untuk meningkatkan kesadaran masyarakat terhadap antibiotik. Resistensi merupakan kemampuan bakteri untuk menghilangkan ataupun melemahkan daya kerja antibiotik. Munculnya kemampuan bakteri, khususnya Escherichia coli untuk bersifat resisten terhadap penggunaan senyawa antibiotik tentunya menimbulkan masalah yang besar bagi manusia, hewan, dan lingkungan. Kecamatan Narmada yang ada di Lombok Barat merupakan salah satu Kecamatan yang memiliki populasi ternak ayam yang cukup banyak, salah satu penyakit infeksi yang sering meyerang unggas dan diobati menggunakan antibiotik adalah Escherichia coli. Penelitian ini bertujuan mengatahui data resistensi antibiotik pada Escherichia coli, data resistensi tersebut dapat digunakan sebagai salah satu upaya pencegahan resitensi antibiotik terhadap bakteri Escherichia coli di peternakan ayam layer di desa Sesaot Kabupaten Lombok barat. Jenis penelitian deskriptif. Dengan menggunakan delapan isolat Escherichia coli, diujikan dengan antibiotic Tetracyline, Penicllin G dan Oxytetracyline. Uji resistensi bakteri dilakukan setelah diperoleh hasil dari identifikasi bakteri, bakteri Escherichia coli distreak menggunakan cotton bud dan dioleskan ke media Mueller Hilton Agar (MHA) kemudian disc cakram antibiotik ditempelkan pada media tersebut dan diinkubasi selama 24 jam dengan suhu 37oC pada inkubator. Hasil yang didapatkan dari enam isolat Escherichia coli yang diuji resistensi antibiotik, antibiotik dikategorikan supceptible Tetracycline (0.0%), Penicillin G (0.0%), dan Oxytetracycline (0.0%). Antibiotik dikategorikan intermediet terdiri dari Tetracycline (33.3%), Penicillin G (0.0%), dan Oxytetracycline (0.0%). Kemudian antibiotik dikategorikan resisten terdiri dari Tetracycline (66.6%), Penicillin G (100%), dan Oxytetracycline (100%).
A CYTOKINE RESPONSE IN BRUCELLA ABORTUS BOVINE INFECTION: LITERATURE REVIEW septyana eka rahmawati; RIAN ANGGIA destiawan; novarina sulsia ista'in ningtyas; Mutia Hariani Nurjanah
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.1-12

Abstract

Brucella Abortus infection in cattle is an infection that has a strategic effect on society, especially the economic sector. This disease is caused by gram-negative bacteria, namely Brucella abortion. Treatment of Brucella Abortus Infection in cattle is still not effective. Therapy based on the immune response caused by Brucella Abortus Infection, especially the cytokine response, is an effective choice considering that each type of cow has a different immune response in dealing with Brucella Abortus Infection. The purpose of writing this literature review is to identify cytokines that play a role in Brucella Abortus Infection in cattle. Method: automated PUBMED database search using keywords “Brucella Abortus Bovine”, “Cytokine”, and “Brucellosis”. Results: 11 articles that are relevant and meet the inclusion criteria to analyze the cytokines that play a role in Brucella Abortus Infection in cattle. Conclusion: Cytokines that play a role in Brucella Abortus infection in cattle are generally IL-1, IL-6, IL-12, IL-8, TNF-α, and IFN-γ.
DETECTION Escherichia coli IN DRINKING WATER SOURCES IN CHICKEN FARMING IN NARMADA DISTRICT, LOMBOK BARAT REGENCY novarina sulsia ista'in ningtyas; alfiana laili dwi agustin; septyana eka rahmawati
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.74-83

Abstract

Water plays an important role in human life. One of the sources of water used by the community is a source of water that comes from wells. The water source in the poultry area in Narmada is not only used for livestock needs but is utilized and consumed by the community especially farmers around the water source. The distance between the water source and the poultry area is less then 15 meters which is the cause of contamination. Research the aimed to detect contaminating bacterial in water source in Poultry of Narmada Regency. The study used a survey method with cross sectional study approach which was carried out in September 2022. Sample were taken using sterile bottles which were then cultured in EMBA, NA and biochemical test. The result of this study showed that there were four samples contaminated with E.coli which was marked with a metallic green on EMBA and the show basil and red colors under microscope.
POTENTIAL OF SEAWEED (Eucheuma cottonii) EXTRACT AS A HEPATOPROTECTOR IN RABBIT (Oryctolagus cuniculus) INDUCED ORGANOPHOSPHATE PESTICIDES Alza Hamdi Putra; kholik kholik; novarina sulsia ista'in ningtyas
Jurnal Biosains Pascasarjana Vol. 24 No. 1SP (2022): JURNAL BIOSAINS PASCASARJANA: SPECIAL ISSUE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v24i1SP.2022.21-29

Abstract

The use of organophosphate pesticides in developing countries is already widespread and can contaminate animal feed or human food ingredients which has an impact on liver damage and other organs. The use of seaweed containing polyphenols as a hepatoprotector for organophosphate poisoning has not been widely used by the public. The purpose of this study was to determine the potential of seaweed extract as a hepatoprotector in organophosphate-induced rabbits as seen from serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) levels. This study used a complete randomized design with 20 male rabbits as experimental animals which were divided into 4 treatments with 5 repetitions, namely: P0 was the control group which was only given distilled water, P1 was the group given seaweed extract at a dose of 200 mg/Kg BW, P2 is the group given seaweed extract at a dose of 400 mg/KgBB and P3 is the positive control group which is not given seaweed extract and distilled water. On the 15th day the P1, P2, and P3 groups were given profenofos pesticide at a dose of 50 mg/kg BW. The results showed a very significant difference (p-value = 0.02, α = 0.05) in SGOT levels between the P0 control group (135.4 ± 67.00 U/L) and the P1 treatment group (41.8 ± 17.45 U/L), P2 (25.8 ± 8.75 U/L), and the positive control group P3 (70 ± 29.04). The results of the study also found that there was no significant difference in SGPT levels in the P0 control group and the P1, P2, and P3 control groups. Giving seaweed extract with different doses provides a hepatoprotector effect by reducing the release of SGOT into the blood.
Co-Authors . Kholik Alfiana Laili Dwi Agustin Alfiana Laili Dwi Agustin Alza Hamdi Putra Kunti Tirtasari Mutia Hariani Nurjanah Rahmawati, Septyana Eka RIAN ANGGIA DESTIAWAN