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All Journal Folia Medica Indonesiana Medula
Yuyun Wirasasmita
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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NEUROGENIC MODULATION BY NEUROKININ-1 RECEPTOR ANTAGONIST, CP-96,345 TO INHIBIT RHEUMATOID ARTHRITIS DEVELOPMENT IN ADJUVANT INDUCED ARTHRITIS RAT MODEL Yuyun Wirasasmita; Mahardian Rahmadi; Imam Susilo; Junaidi Khotib
Folia Medica Indonesiana Vol. 52 No. 2 (2016): APRIL - JUNE 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.021 KB) | DOI: 10.20473/fmi.v52i2.5216

Abstract

Rheumatoid arthritis (RA) is a chronic form of persistent inflammation. Meanwhile, Substance P is the most associated neuropeptide in neurogenic inflammation and hyperalgesia commonly found in chronic pain. Substance P act by binding to neurokinin-1 receptor. The present study was conducted to evaluate the effect of neurokinin-1 receptor antagonist (CP-96,345) on Adjuvant Induced Arthritis rat model, induced by Complete Freund’s Adjuvant (CFA). The objective is to attenuate neurogenic inflammation which in turn will increase the latency time of hyperalgesia response, decreases neurokinin-1 receptor expression, and inhibits the development of RA in AIA rat model. Rats were intra-articularly injected with CFA 1 hour after the administration of CP-96,345 either by 0.63 µg/gr; 1.25 µg/gr; or 2.5 µg/gr also intra-articularly. Caliper measurements and hot-plate test were performed on day 0, 3, 5, 7, 9, 11, and day 13. Expression of neurokinin-1 receptor in joint tissue were evaluated by immunohistochemistry, and RA progress in joint tissue were observed hystopathologically. CP-96,345 at 2.5 µg/gr significantly increases the latency of hyperalgesia response time on CFA induced rats (p=0.044) and decreased the neurokinin-1 receptor expression in joint tissue (p=0.029) compared to CFA induced rats. There was no significant difference for caliper measurements and RA progress between CFA incduced rats and treated group. Conclusively, CP-96,345 increases the latency of hyperalgesia response time and decreases the NK-1 receptor expression in rat joint but could not inhibit RA progression.
The Formulation and Antioxidant Activity Test of Binahong (Anredera cordifolia) Leaves Extract Lotion Hasty Hamzah; Yuyun Wirasasmita; Wa Ode Syafriah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 9, No 2 (2022)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46496/medula.v9i2.24014

Abstract

Background: With current wide range of scientific research regarding the benefits of antioxidants, many has tried to invent new sources of this compound. The use of plant-based source for antioxidants recently become more and more popular, and one of these plants is Binahong (Anredera cordifolia). The leaves of these plant had been analyzed before to contain antioxidant compound. Objective: This research aimed to test, based on previous claim of antioxidant activity of Binahong (Anredera cordifolia) leaves. Methods: The lotion used was formulated using Tween 60 and Span 60 as its emulgator. After test, it was determined that the emulsion type is water in oil (w/o). Before Binahong Leaves extract were incorporated, the lotion was tested for physical stability including organoleptics, emulsion type, pH determination, homogeneity, and viscosity. Results: The analysis for antioxidant activity by DPPH method of Binahong leaves extract with various concentrations of 12,5 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, yield a value of IC50 822.22 µg/mL (mild antioxidant activity level). This result corroborates previous findings with the same result. Meanwhile, the lotion containing Binahong leaves extract with various concentrations of 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL were analyzed with same method and yield a value of IC50 27041.50 µg/mL. Conclusion: The result proved there was a further loss of antioxidant activity after the extract were incorporated into lotion. Keywords: Formulation, Anredera cordifolia, Antioxidant, Antioxidant Lotion, Binahong, 
Co-Authors Hasty Hamzah Imam Susilo Junaidi Khotib Mahardian Rahmadi Syafriah, Wa Ode