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Tomohiro Kotaki
Center for Infectious Disease, Kobe University Graduate School of Medicine, Japan
Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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Molecular Surveillance of Dengue Virus Serotype Using Polymerase Chain Reaction in Surabaya 2013 Teguh Hari Sucipto; Amaliah Labiqah; Siti Churrotin; Nur Ahwanah; Kris Cahyo Mulyatno; Soegeng Soegijanto; Tomohiro Kotaki; Masanori Kameoka; Eiji Konishi
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 1 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.228 KB) | DOI: 10.20473/ijtid.v5i1.207

Abstract

Dengue is one of the infectious diseases which is endemic in the tropical and sub-tropical country. The disease found in Indonesia Surabaya, 1968. The symptoms of Dengue virus infections are two kinds, first DF (Dengue Fever), second DHF (Dengue Hemorrhagic Fever). This infectious disease transmitted by Aedes aegypti mosquito. Mosquitoes breed in clean water areas. More than 100,000 cases of DF/DHF ccurred in Indonesia every year. The purpose of this study were to provide information and the spread of dengue virus types in Surabaya from January 2013 to September 2013. The nalysis technique used to determine the type of dengue virus nfectionwas used PCR (Polymerase Chain Reaction). The results obtained 69% DENV-1, 27% DENV-2 isolates, 4% isolates DENV-3, and 0% DENV-4 isolates.
ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL Teguh Hari Sucipto; Siti Churrotin; Harsasi Setyawati; Tomohiro Kotaki; Fahimah Martak; Soegeng Soegijanto
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 4 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.829 KB) | DOI: 10.20473/ijtid.v6i4.3806

Abstract

Infection of dengue virus (DENV) was number of globally significant emerging pathogen. Antiviral dengue therapies ar importantly needed to control emerging dengue. Dengue virus (DENV) is mosquito-borne arboviruses responsible for causing acute systemic diseases and grievous health conditions in humans. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Copper and copper compounds have more effective in inactivation viruses, likes an influenza virus and human immunodeficiency virus (HIV). Purpose in this project was investigated of Copper(II)chloride Dihydrate antiviral compound were further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measures by Enzyme linked Immunosorbent Assay (ELISA) with selectivity index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for compound. The maximal inhibitory concentration (IC50) of Copper(II)chloride Dihydrate against dengue virus type-2 was 0.13 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was 5.03 μg/ml. The SI values for Copper(II)chloride Dihydrate 38.69. Result of this study suggest that Copper(II)chloride Dihydrate demonstated significant anti-DENV-2 inhibitory activities and not toxic in the Vero cells. Copper mechanisms play an important role in the prevention of copper toxicity, exposure to excessive levels of copper can result in a number of adverse health effects, as a result increased reactive oxygen species and oxidative damage to lipid, DNA, and proteins have been observed in human cell culture models or clinical syndromes of severe copper deficiency and inhibition was attributed to released cupric ions which react with cysteine residues on the surface of the protease.
Co-Authors Amaliah Labiqah Eiji Konishi Fahimah Martak Harsasi Setyawati Kris Cahyo Mulyatno Masanori Kameoka Nur Ahwanah Siti Churrotin Siti Churrotin Soegeng Soegijanto Teguh Hari Sucipto Teguh Hari Sucipto