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All Journal Microbiology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA)
Nining Betawati Prihantini
Department of Biology, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok 16424, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics

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The Role of Indonesian Indigenous Cyanobacteria Culture Collection as An Ex-situ Conservation Effort and Microalgae Biodiversity Study Material Nining Betawati Prihantini
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 3 (2023): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i3.2763

Abstract

Exploratory research has been carried out on Indonesian indigenous Cyanobacteria in an effort to collect these isolates to be used as learning materials for microalgae biodiversity. Cyanobacteria are known as photosynthetic bacteria and prokaryotic microalgae. The members of the cyanobacteria found were isolated, cultured, identified, and collected. The location for sampling cyanobacteria is the UI Depok campus area which consists of water from small lakes (situ) and soil. The results showed that 22 strains of Cyanobacteria were isolated and cultured properly, and consisted of 12 genera/species, namely Merismopedia sp. (1 isolate), Synechococcus sp. (1 isolate), Limnothrix redekei (1 isolate), Oscillatoria sp.1 (1 isolate), Oscillatoria sp.2 (1 isolate), Pseudanabaena sp. (3 isolates), Nostoc sp. (4 isolates), Anabaena sp. (1 isolate), Scytonema sp. (1 isolate), Fischerella sp. (1 isolate), Stigonema sp. (1 isolate), and Hapalosiphon sp. (6 isolates). Twenty-two (22) strains were then collected and treated by subculture method. Of the 11 genera found, there are 3 genera that have been known as genera that have potential as sources of biobased materials, biofuels, biofertilizers, and others, namely Synechococcus, Nostoc, and Anabaena
Influence of Temperature Variations on Growth of Nostoc (Cyanobacteria) HS-5 and HS-20 Isolated from Indonesian Hot Springs NINING BETAWATI PRIHANTINI; CAHYA GUSLYANI; RATNA YUNIATI; WELLYZAR SJAMSURIDZAL
Microbiology Indonesia Vol. 12 No. 2 (2018): June 2018
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1761.766 KB) | DOI: 10.5454/mi.12.2.1

Abstract

The research aims to know the effect of variation temperature to the growth of Nostoc HS (Hot Spring)-5 and HS-20. Strain of Nostoc HS-5 was isolated from Ciseeng hot spring which has habitat temperature range of 30-43 °C, and Nostoc HS-20 was isolated from Pancar Mountain hot spring which has temperature range of 46-69 °C. The research was done by measuring biomass weight and chlorophyll content on day-1, 2, 3, 4, 7, 10, 14, 17, and 21. The temperatures used were 20 °C, 35 °C, and 50 °C. The growth medium used was Bold Basal Medium (BBM) with pH 6.6. Each treatment was made in four replications. Non-parametric statistical analysis used were the Friedman test (a=0.05) and Spearman test (a=0.01).The result showed there were significant differences on the biomass weight of Nostoc HS-5 and HS-20 grown at temperature of 20 °C, 35 °C, and 50 °C. The average amount of biomass highest weight for Nostoc HS-5 and HS-20 occurred in both strains were grown at 35 °C.  Besides that, there was no correlation between the weight of biomass and chlorophyll content of Nostoc HS-5 and HS-20.
Co-Authors Arya Widyawan Berta Putri CAHYA GUSLYANI Dian Hendrayanti Dini Damayanti Ratna Yuniati Ronny Rianto Wellyzar Sjamsuridzal Wisnu Wardhana Yuni Ariyani