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COMBINATION OF DECIDUAL DENTAL STEM CELL AND PLATELET RICH PLASMA IN FIBROBLAST FORMATION IN THE JAWBONE REGENERATION OF WISTAR RATS Ade Ismail; Rahmawati Sri Praptiningsih; Muhammad naufal
ODONTO : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.0.155-161

Background: Orthodontic tooth movement is caused by mechanichal forces that cause pseudo-inflammation with the result that FGF (Fibroblast Growth Factors) was activated. The combination of SHED (Stem Cell From Human Exfoliated Deciduous Theeth) and PRP (Platelet Rich Plasma) hydrogels can affect the formation of fibroblast in the alveolar bone regeneration. The purpose of this study is the effect determination of SHED and PRP hydrogels for fibroblast formation in alveolar bone regeneration in wistar rats.Method: An experimental study was held using post-test only control group design. There were four groups, namely the PRP hydrogel group, the SHED hydrogel group, the SHED and PRP hydrogel group, and the povidone iodine group (n=8) treated with orthodontic tooth movement. Their mandibular tissue was made as histologycal slide, and the FGF expression were observed microscopically with 100x magnification. The data analyzed by ANNOVA and LSD test.Result: There was a significant effect of the combination of SHED and PRP hydrogel in ordering fibroblast in alveolar bone regeneration by ANNOVA test 0.001 (<0.05). This is because SHED and PRP are able to stimulate FGF expression through external stimulation.Conclusion: It concluded that the combination of SHED and PRP hydrogel affected fibroblast formation on alveolar bone regeneration in wistar rats

The Effectiveness of α-Mangostin in Reducing the Streptococcus Mutans Biofilm Thickness Rahmawati Sri Praptiningsih; Arlina Nurhapsari; Rifqi Wahyu Febrian
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 11, No 2 (2022): November
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v11i2.15809

Dental caries occurs due to the demineralization of tooth structure caused by microorganisms in colonies called biofilms. One of the microorganisms involved in dental caries is Streptococcus mutans. Oral mouthwash, in addition to mechanical cleaning, is known to prevent the growth of oral microorganisms. Mangosteen is known as an anti-cancer ingredient with high anti-bacterial properties. This study aims to identify the effectiveness of mangosteen skin extract (Garcinia mangostana L.) in decreasing Streptococcus mutans biofilm. The study is experimental research with a post-test control group design. The research sample was divided into five groups; 3 treatment groups and 2 control groups. The biofilm thickness test was carried out with OD (Optical density) with a wavelength of about 620 nm using an ELISA reader. Kruskal Wallis analysis was employed as a non-parametric statistical test analysis. Statistical Kruskal Wallis indicated significant differences in the thickness of 5 test groups of Streptococcus mutans. The lowest average yield of biofilm thickness was in the α-mangosteen group at 12.5 g/ml. Conclusion: α-mangosteen in mangosteen skin extract effectively reduced the thickness of Streptococcus mutans biofilm.

The effect of brown algae (Sargassum sp.) gel on the number of osteoclasts in periodontitis rats Muhamat Muhtar S. Abdurrohman; Rahmawati Sri Praptiningsih; Yulistinawati Yulistinawati
Padjadjaran Journal of Dentistry Vol 34, No 3 (2022): November 2022
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no3.39285

ABSTRACTIntroduction: Alveolar bone resorption in periodontitis is caused by increased activation of osteoclasts, causing an imbalance of bone remodeling. Periodontitis treatment can be done mechanically with Scaling and Root Planing and accompanied by metronidazole. Antibiotics have long-term drawbacks and can cause allergies, thus requiring an alternative to natural topical drugs such as brown algae gel. This study aimed to to analyzed the effect of brown algae gel (sargassum sp) on the number of osteoclasts of alveolar bone in periodontitis rats. Methods: The research method was a True Experimental with a pretest-posttest-only control group design. Rats were divided into three groups; negative control (K-) was not given any treatment, positive control (K+) was given metronidazole ties plus 25% concentration, and the treatment group (KP) was given brown algae gel (Sargassum sp) 75% concentration. Data were analyzed by Friedman and Kruskal Wallis test. Results:There was a significant difference in the number of osteoclasts on days 8, 10, and 12, the decrease in the number of osteoclasts was higher in the brown algae treatment group than the negative control group (p=0.051). In the positive control group, there was a significant difference in the decrease of the number of osteoclasts compared to the brown algae treatment group (p=0.029). The number of osteoclasts was significantly different on day 12 (p=0.026).Conclusion: Adhering brown algae (Sargassum sp.) gel decreases osteoclasts in periodontitis rats.Keywords: bone remodeling; bone resorption; brown algae gel (sargassum sp.); osteoclasts; periodontitis.

COMPARISON OF THE EFFECTIVENESS OF FERACRYLUM 1% AND FERACRYLUM 4% IN STOPING BLOODING AFTER GINGIVA INCISION Adilah Tsamarah; Erwid Fatchur Rahman; Rahmawati Sri Praptiningsih
Jurnal Medali Vol 4, No 2 (2022): Media Dental Intelektual September 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.2.163-169

Background: Bleeding is often found in dental practice. Excessive bleeding can hinder wound healing. For the surgeon to achieve rapid and effective hemostasis is important. Therefore, a topical hemocoagulation solution is given to help control surface bleeding by applying it locally. One such topical agent is Feracrylum. Feracrylum is a polymer of polyacrylic acid containing 0.05 to 0.5% iron. Feracrylum iron element will react with albumin and then convert water-soluble fibrinogen into water-insoluble fibrin which then forms a coagulum. This study aims to compare the effectiveness of Feracrylum 1% and Feracrylum 4% in stopping bleeding after gingival incision.Method: This study is a true experimental study that includes 3 treatment groups, namely the group without being given hemostatic agents, the group being given Feracrylum 1%, and the group being given Feracrylum 4%, each group consisting of 9 rats. Data were collected by calculating the bleeding time from the start of bleeding until the bleeding stopped after the gingival incision using a stopwatch and then recorded. Data analysis using Shapiro-Wilk, homogeneity test using Levene Test, nonparametric test using Kruskal-Wallis and Mann Whitney.Result: The results showed that the average bleeding time in the group without hemostatic agents, the group receiving 1% Feracrylum, and the group receiving Feracrylum 4% was 221 seconds, 103 seconds, and 50 seconds. The results of the Kruskal-Wallis and Mann Whitney showed that there was a significant difference in bleeding time between groups.Conclusion: The conclusion of this study is that the application of Feracrylum 4% was faster in stopping bleeding compared to the application of Feracrylum 1% after gingival incision in wistar rats.

COMPARISON OF THE NUMBER OF MACROPHAGES IN THE RAT REVERSIBLE PULPITIS AFTER APPLICATION OF BIODENTIN AND SIWAK EXTRACT Andina Rizkia Putri Kusuma; Rahmawati Sri Praptiningsih; Arlina Nurhapsari
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.6-11

Background: Oral disease still need special attention, especially in dentistry. Inflammation of the pulp is caused by the presence of irritants. One of the mechanical irritants is iatrogenic factors caused by operator error during preparation so that roof of pulp chamber is exposed. This condition is called reversible pulpitis/inflammation of the pulp. Cells that play a role during inflammation are macrophages which are the second defense cell after neutrophil apoptosis. A direct pulp capping procedure is indicated for this condition. The material commonly used is biodentin, but because the price tends to be expensive, alternative materials are needed. Siwak contains flavonoids which are known to play an important anti-inflammatory role in the healing of exposed pulp. The purpose of this study was to compare the number of macrophages in the dental pulp of rats with reversible pulpitis after administration of biodentin and siwak extract.Method: This research is a true experimental with a post test only group design, consisting of 2 treatment groups, namely the 75% siwak extract group and the biodentin group. The material is applied after the tooth has been prepared. The research sample used male wistar rats and sacrificed on the 3rd day. Samples were stained with Hematoxylin Eosin to see macrophage cells.Result: The data obtained were analyzed using the independent T-test. The test results showed a significant difference, with a value of p = 0.008 (p <0.05).Conclusion: The conclusion of this study that the siwak extract group showed a lower mean number of macrophage cells than the biodentin group.

COMBINATION OF DECIDUAL DENTAL STEM CELL AND PLATELET RICH PLASMA IN FIBROBLAST FORMATION IN THE JAWBONE REGENERATION OF WISTAR RATS Ade Ismail; Rahmawati Sri Praptiningsih; Muhammad naufal
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Orthodontic tooth movement is caused by mechanichal forces that cause pseudo-inflammation with the result that FGF (Fibroblast Growth Factors) was activated. The combination of SHED (Stem Cell From Human Exfoliated Deciduous Theeth) and PRP (Platelet Rich Plasma) hydrogels can affect the formation of fibroblast in the alveolar bone regeneration. The purpose of this study is the effect determination of SHED and PRP hydrogels for fibroblast formation in alveolar bone regeneration in wistar rats.Method: An experimental study was held using post-test only control group design. There were four groups, namely the PRP hydrogel group, the SHED hydrogel group, the SHED and PRP hydrogel group, and the povidone iodine group (n=8) treated with orthodontic tooth movement. Their mandibular tissue was made as histologycal slide, and the FGF expression were observed microscopically with 100x magnification. The data analyzed by ANNOVA and LSD test.Result: There was a significant effect of the combination of SHED and PRP hydrogel in ordering fibroblast in alveolar bone regeneration by ANNOVA test 0.001 (<0.05). This is because SHED and PRP are able to stimulate FGF expression through external stimulation.Conclusion: It concluded that the combination of SHED and PRP hydrogel affected fibroblast formation on alveolar bone regeneration in wistar rats

The Effectiveness Of Black Cumin Nanoemulsion Gel Concentration 10% And 15% On Staphylococcus Aureus Biofilm Thickness (In Vitro) Rahmawati Sri Praptiningsih; Anggun Feranisa; Jafifah Mega Nur Istighfah; rizqi Amalina
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.136-145

Background: Periodontal disease is a disease that often occurs in the oral cavity with prevalence in Semarang City increasing in 2016 by 67.18% to 89.53%. One of the causes of periodontal disease is the buildup of Stapylococcus aureus bacterial biofilm during the initial colonization of the tooth pellicle formation. One herbal ingredient that can be used as an antibacterial is black cumin (Nigella sativa). Gel nanoemulsion technology has the advantage of increasing material stability. This research aims to determine the effectiveness of black cumin gel nanoemulsion on the growth of Stapylococcus aureus biofilm thickness. Methods:The treatment group consisted of black cumin gel nanoemulsion with a concentration of 10% and a concentration of 15% and the control group, namely 0.2% chlorhexidine and distilled water. Stapylococcus aureus biofilm thickness was measured using an ELISA-reader. Statistical tests were carried out using the One Way Anova test. Results: The average thickness of Stapylococcus aureus with the addition of black cumin gel nanoemulsion with a concentration of 15% was the lowest, namely 0.046, while the thickness of Stapylococcus aureus with the addition of distilled water was the highest, namely 0.158. The One Way Anova test obtained a significance figure of 0.000 (p<0.05) so it could be concluded that there were significant differences in the 4 Stapylococcus aureus thickness test groups.Conclusion: Black cumin gel nanoemulsion concentrations of 10% and 15% have antibacterial properties which can reduce the thickness of Stapylococcus aureus biofilm.

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