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Pengaruh Pemberian Asam Amino Metionin-Sistin Pada Pakan Yang Terkontaminasi Aflatoxin B1 Terhadap Mortalitas Dan Kinerja Organ Dalam Ayam Broiler Listya Purnamasari; Ali Agus; Cuk Tri Noviandi
Jurnal Ilmu Ternak Vol 20, No 1 (2020): June
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v20i1.27564

Kondisi iklim di Indonesia dengan suhu dan kelembapan yang tinggi merupakan kondisi optimal bagi perkembangan jamur Aspergillus flavus penghasil toksin alflatoksin. Aflatoksin dapat menurunkan produktivitas ternak. Penelitian ini bertujuan untuk mengetahui efek penambahan asam amino metionin-sistin untuk menurunkan efek toksin dari aflatoksin B1 (AFB1) pada pakan ayam broiler terhadap mortalitas dan kinerja organ dalam ayam broiler. Sebanyak 240 ekor ayam broiler mixed sex diteliti dengan 9 macam perlakuan dengan pola rancangan faktorial 3×3 dengan faktor kadar asam amino metionin-sistin (75,100, dan 125%) dan kadar AFB1 (0, 200, dan 400 ppb). Variabel yang diamati adalah bobot karkas, mortalitas, bobot hati dan bobot bursa fabrisius. Hasil penelitian menunjukkan interaksi tidak terjadi bobot relatif hati, bobot relatif bursa fabrisius, bobot relatif karkas. Mortalitas tertinggi terjadi pada perlakuan T3 (M+C 125% dan AFB1 0 ppb). Kombinasi pemberian asam amino metionin-sistin sebesar 75, 100, dan 125% serta AFB1 0, 200 dan 400 ppb yang dikonsumsi ayam broiler, belum mampu memperbaiki kinerja organ dalam ayam broiler.

MPPA (Most Probable Producing Ability) Estimation of Kebumen Ongole Crossbred Cattle based on Offsprings Weaning Weight Sumadi Sumadi; Nono Ngadiyono; Diah Tri Widayati; Cuk Tri Noviandi; Akhmad Fathoni; Mukhamad Khusnudin
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Tujuan dari penelitian ini yaitu untuk menghitung nilai MPPA induk pada sapi PO Kebumen. Penelitian ini telah dilakukan pada bulan Juli sampai dengan Oktober 2015 di wilayah Urut Sewu, Kebumen. Materi penelitian yang digunakan adalah data recording tiga tahun terakhir (2013-2015) terdiri dari 41 ekor pejantan, 51 induk dan 244 pedet. Data berat sapih anak sebelumnya telah dikoreksi berdasarkan umur induk, jenis kelamin dan umur penyapihan 120 hari. Ripitabilitas dihitung menggunakan metode korelasi antarkelas. Nilai MPPA induk dihitung berdasarkan nilai ripitabilitas berat sapih keturunan. Hasil penelitian menunjukkan bahwa nilai ripitabilitas berat sapih anak termasuk dalam kategori tinggi (0.32 ± 0.15). Nilai MPPA induk sapi PO Kebumen kemudian dihitung menggunakan nilai tersebut dan juga rata-rata performan populasi (83.45 kg). Dihasilkan 10 besar induk sapi PO Kebumen dengan nilai MPPA tertinggi. Nilai MPPA kemudian diurutkan dari nilai tertinggi ke terendah. Nilai MPPA tertinggi dimiliki oleh SAJ0315078 (110.69) dan terendah dimiliki oleh TA1112020 (97.20). Hasil dari penelitian ini kemudian dapat digunakan sebagai dasar dalam seleksi induk sapi PO Kebumen di dalam populasinya.

Pengaruh Penggunaan Minyak Lemuru dan Minyak Sawit dalam Ransum terhadap Kinerja dan Kualitas Telur Puyuh Cuk Tri Noviandi; Ali Mas'adi; Zuprizal (Zuprizal); Indratiningsih (Indratiningsih); Tri Yuwanta; Sri Harimurti
Buletin Peternakan Vol 27, No 3 (2003): Buletin Peternakan Vol. 27 (3) Agustus 2003
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v27i3.1475

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ADDITION OF CASSAVA IN THE BASAL FEED BASED ON RICE STRAW FERMENTATION TO IMPROVE THE DIGESTIBILITY IN VITRO ON THE BALI CATTLE John Bernhard Sendow Christofel; Cuk Tri Noviandi; Ristianto Utomo
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.8807

INTISARI Tujuan penelitian untuk mengetahui daya cerna sapi Bali secara in vitro terhadap penambahan ubi kayu pada waktu yang berbeda terhadap pakan basal jerami padi fermentasi. Penelitian ini selama 4 bulandan dilaksanakan di Laboratorium Teknologi Makanan Ternak, Biokimia Nutrisi, Uji Teknologi Pangan dan Hasil, Universitas Gadjah Mada, Yogyakarta. Persiapan alat dan bahan yakni sapi Bali berfistula rumen, jerami padi fermentasi, ubi kayu, seperangkat bahan dan alat laboratorium untuk analisis proksimat in vitro.Uji in vitro tahap pertama untuk mengetahui kecernaan bahan kering (BK), bahan organik (BO), konsentrasi NH3, protein mikrobia dan VFA, penelitian menggunakan metodein vitroTilley dan Terry dengan memodifikasi pada bagian tutup tabung reaksi. Data dianalisis dengan menggunakan analisis variansi completely randomized design (CRD) one-way ANOVA dengan bantuan XLSTAT. Analisis dilanjutkan menggunakan Duncan’s new multiple range test (DMRT) jika terdapat perbedaan. Hasil uji in vitro menunjukkan perbedaan nyata (P<0,05) dengan perlakuannya yaknitanpa penambahan 10% (0 jam) danpenambahan 10%ubi kayu kering pada jam ke 2, 4, dan 6sebagai berikut BK(35,35%, 46,01%, 45,27%,41,52%). BO (44,74%, 53,57%, 50,49%, 51,04%),NH3 (6,77 mg/100 ml, 4,85 mg/100 ml, 5,66 mg/100 ml, 5,39 mg/100 ml), protein mikrobia (0,17 mMol/ml/g, 0,15 mMol/ml/g, 0,14 mMol/ml/g, 0,14 mMol/ml/g), VFA : asetat, (22,98 mM, 20,18 mM, 30,70 mM, 35,21 mM), propionat (5,51 mM, 4,87 mM, 5,40 mM, 7,46 mM), butirat (1,38 mM, 2,51 mM, 3,23 mM, 3,42 mM). Kesimpulannya yakni penambahan ubi kayu pada waktu berbeda setelah diberi pakan basal jerami padi fermentasi dapat meningkatkan kecernaan sapi Bali secara in vitro. (Kata Kunci : In Vitro, Jerami Padi Fermentasi, Sapi Bali, Ubi Kayu).

Kajian Produksi Aflatoksin B1 Kasar dari Isolat Kapang Aspergillus Flavus Lokal pada Media Jagung dan Jagung+Kacang Tanah listya purnamasari; Ali Agus; Cuk Tri Noviandi
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9354

Aflatoxin, which known as toxigenic compound, is a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin is found in both food and feed stuffs, such as: corn and peanut. Aflatoxin standard is needed in every research of aflatoxin contamination. But it is rather difficult to get. It is imported, high costs, and take times. The aim of the research is to assess the potential local isolate of A. flavus to producing aflatoxin that can be used to be the alternative aflatoxin standard. Local mold of A. flavus FNCC 6122 and FNCC 6109 are got from PAU University of Gadjah Mada. Isolate was enriched on PDA medium for 5 days and move to corn medium of corn+peanut combine medium for 10-15 day at 25ºC. The variables was the content of AFB1 by ELISA test. The result is shown that isolat FNCC 6122 produced higher AFB1 than isolate FNCC 6109. The combine of corn and peanut medium stimulated FNCC 6122 to produce higher AFB1 and has potency to be the candidate of standard aflatoxin. In conclusion, the peanut addition on the medium would initiate increasing of the aflatoksin B1 level.

Growth, Production and Seed Quality of Brachiaria brizantha cv. Mg 5 Under Different Planting Space Nafiatul Umami; Asrie Hardianing Indra Kusuma; Cuk Tri Noviandi
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.12803

The research aimed to investigate the effect of different planting space on the seed production of Brachiaria brizantha cv. MG 5.This study used three dimensions of spacing: 75 x 75 cm, 100 x 100 cm, and 150 x 150 cm. The seed was germinated before planted on the 3 x 3 m plot with three replications. Fertilization was performed twice at the age of 30 and 90 days with NPK fertilizer 400 kg ha-1 and given the same amount of water. The variables observed were the plants’ height, length, number of tiller, inflorescence, raceme, spikelet, the seed purity, the seed production (kg ha-1), and viability. The data obtained were analyzed statistically by using the Completely Randomized Design (CRD), and if any difference existed, it was continued with Duncan's new multiple range test. The results showed that the different planting space was significant (P<0.05) to the seed production of Brachiaria brizantha cv. MG5., which was the seed production at 75 x 75 cm spacing was 206.43 kg ha-1, 100 x 100 cm was 354.43 kg ha-1, and 150 x 150 cm was 128.87 kg ha-1. The highest seed production was resulted in planting space of 100 x 100 cm that was 354.43 kg ha-1.

Effects of Methionine-Cysteine Amino Acid Supplementations in the Aflatoxin B1 Contaminated Diet on Broiler Production Performance Listya Purnamasari; Ali Agus; Cuk Tri Noviandi
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i4.31150

This research aimed to observe the interaction of methionine-cysteine amino acid supplementation to decrease the effect of aflatoxin B1 (AFB1) on diet against production performance of broiler chicken. A number of 240 mixed sex broiler chickens were treated in 9 treatments by factorial design 3 x 3 with methionine-cysteine amino acid (M+C) (75,100, dan 125%) factors and AFB1 levels (0, 200, dan 400 ppb). Variables observed were: Weight gain, feed consumption, and feed conversion ratio (FCR). The results showed that increased AFB1 content in diet from 0 to 400 ppb increased chicken body weight (P <0.05) in each age group. The high body weight was balanced with high feed consumption along with increased nutrient needs, mainly sulfuric amino acid (M+C) as the precursor of glutathione to eliminate toxic through conjugation reactions. The interaction effect was firstly occurred between M + C and AFB1 treatment (P <0.05). Meanwhile increased supplementation of M + C from 75 to 125% caused decreased feed consumption in each age group of chickens, but increased AFB1 levels further increased feed consumption (P<0.05). The interaction effect between the level of M + C and AFB1 contamination in diets on feed consumption were seen in 21-day-old chickens (P<0.05). FCR was also increased (P <0.05) with the reduction of M + C content in diet at 7 days old. The effect of AFB1 on diet and interaction between M + C and AFB1 on chicken FCR in this study was not significant in all age groups. It can be concluded from the current study that supplying methionine-cystine amino acid with 75, 100 and 125% in AFB1 contaminated diet of 0, 200 and 400 ppb improves the performance of broiler chicken production.

Nutrient Quality and in Vitro Digestibility of Bio-Ammoniated Rice Straw Bales Cuk Tri Noviandi; Dibya Ratnopama; Ali Agus; Ristianto Utomo
Buletin Peternakan Vol 44, No 3 (2020): BULETIN PETERNAKAN VOL. 44 (3) AUGUST 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i3.53409

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.

Feed Evaluation Based on in Vitro Gas Production of Tropical Forages with Addition of Different Polyethylene Glycol (Peg) Level Widya Kenshiana Putri; Cuk Tri Noviandi; Kustantinah Adiwimarta
Buletin Peternakan Vol 45, No 1 (2021): BULETIN PETERNAKAN VOL. 45 (1) FEBRUARY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i1.58433

This study aimed to determine the chemical composition and gas production based on fermentation in the rumen of nine types of forage tropical feed commonly used in Indonesia. The forages used in this study were: mahogany leaves (Swietenia mahagoni L. Jacq.), tayuman leaves (Bauhinia purpurea), bamboo leaves (Bambusa arundinaceae), canary leaves (Canarium indicum L.), tea leaves (Camellia sinensis), ketapang leaves (Terminalia catapa L.), lamtoro leaves (Leucaena leucocephala), tehtehan leaves (Duranta repens), and turi leaves (Sesbania grandiflora). Measurements of in vitro gas production were carried out at 10 observation points (2, 4, 6, 8, 12, 16, 24, 36, 48, 72 hour). Tannin activity was measured for each sample at observation points using measurements of gas production divided into three groups with modified levels of polyethylene glycol (PEG), ie samples without PEG (P1); sample + PEG in the amount of 200 mgDM (P2); and PEG + samples of tannins contained in each forage based on literature studies (P3). Data were analyzed for a variance by following the factorial completely randomized design (CRD) pattern. Statistical analyzes were performed on all data by following the general linear procedure in PROC GLM from SAS. The data obtained were analyzed for variance at the 5% significance level. The results of gas production calculations showed that crude protein (CP) from each forage ranges from 5.75 - 22.37% where the highest CP was owned by turi leaves (S. grandiflora). The content of crude fiber (CF) ranged from 5.30 - 20.93%. The most optimal measurement of gas production was in the sample given PEG in the amount of 200mg/kg with a significant difference (P<0.05). The higher of the tannin content contained in the forage, the lower of gas produced. Measurement of tannin content showed that condensed tannin content varied from turi leaves by 0.20% to the highest in mahogany leaves by 8.60%. The addition of 200 mg/100mgDM of PEG optimizes the rate of forage gas production, especially for grass plants (gramineae).

Physiological Response on Broiler Chicken’s Liver Supplemented Amino Acid Metionine-Cystine in Feed Contaminated with Aflatoxin B1 Listya Purnamasari; Ali Agus; Cuk Tri Noviandi
Journal of Livestock Science and Production Vol 3, No 1 (2019): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v3i1.1375

The content of AFB1 in feed with low levels and spend a long time, will cause primary damage or primary liver carcinoma. This study aims to reduce the toxicity of AFB1 with amino acid methionine-cystine supplementation in broiler chicken feed. This study used a 3 × 3 factorial design with methionine-cystine amino acid levels (M + C: 75, 100 and 125%) and AFB1 levels (0, 200, and 400 ppb). The variables collected were liver physiology, liver histopathology, SGPT levels, and SGOT levels. Observations of liver physiology showed that feed containing aflatoxin without methionine-cystine amino acid had a paler yellowish color (T4, T7 and T8). Pathological examination resulted that aflatoxicosis will attack the liver. Transition amino acid cystines in chicken feed contaminated with AFB1 did not occur in blood SGPT levels. Blood SGOT levels were highest at 21 days of age, namely T2 (M + C 100%) and T3 (M + C 125%) at AFB1 0 ppb which showed excess liver damage. The administration of methionine-cystine amino acids of 75, 100 and 125% in chicken feed contaminated with AFB1 0, 200 and 400 ppb consumed by broilers carries a risk of physiological and pathological damage to chicken liver.

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