Article Per Year (5 Year)
p-Index From 2019 - 2024
1.259
P-Index
This Author published in this journals
All Journal Jurnal Biologi Udayana Jurnal Tumbuhan Obat Indonesia Chimica et Natura Acta Indonesian Journal of Chemistry Pharmacology and Clinical Pharmacy Research Acta Biochimica Indonesiana Nusantara Science and Technology Proceedings Jurnal Kesehatan Bakti Tunas Husada
Irma Mardiah
Department Of Biotechnology Pharmacy, Indonesian School Of Pharmacy, Bandung, 40266, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

Published : 9 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 9 Documents
Search

 1 
Potensi Bacillus cereus dalam produksi biosurfaktan Nur Asni Setiani; Nia Agustina; Irma Mardiah; Syarif Hamdani; Dewi Astriany
Jurnal Biologi Udayana Vol 24 No 2 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2020.v24.i02.p09

Abstract

Biosurfactants from microorganisms have stable physical and chemical properties, environment friendly, biodegradable, renewable, cheaper and low toxicity that can be used in the pharmaceutical industry as antibacterial. The aim of this study was to examine the emulsification and characterization of biosurfactant from Bacillus cereus. Hemolysis and spreading oil tests was performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. The supernatant was measured for biosurfactant activity through an emulsification index and characterized using thin layer chromatography and antibacterial qualitative testing. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44,83%, 48,28%, 51,72%, 46,43%, and 51,50%. The formation of red stains on TLC plates with ninhydrin appearance showed lipopeptide groups. Antibacterial testing using Escherichia coli and Staphylococcus aureus showed the formation of clear zones around the disk paper. Bacillus cereus can produce biosurfactants of the lipopeptide group with the largest emulsification index of 51.72% and has antibacterial activity against both gram positive and gram negative.
AKTIVITAS PENURUNAN TEKANAN INTRAOKULAR KOMBINASI EKSTRAK ETANOL BUAH DELIMA DAN LIDAH BUAYA Novi Irwan Fauzi; Irma Mardiah
Jurnal Tumbuhan Obat Indonesia Vol 14 No 2 (2021): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v14i2.4120

Abstract

ABSTRACT Glaucoma is the second leading cause of blindness in the world. One of many strategies for controlling intraocular pressure to prevent blindness due to glaucoma is by inhibiting the production and/or increasing drainage of fluid in the eyeball. One of the potential natural remedies for such control is pomegranate (Punica granatum L.) and aloe vera (Aloe vera L.). This study aimed to evaluate the activity of lowering intraocular pressure of pomegranate and Aloe vera combination ethanol extracts in rats with ocular hypertension. Pomegranate and aloe vera were extracted by maceration method using 70% ethanol. Thirty rats were randomly divided into six groups, namely normal control group, rats with ocular hypertension control, drug control (acetazolamide 25 mg/kg BW p.o), pomegranate extract 500 mg/kg BW p.o, Aloe vera extract 500 mg/kg BW p.o, and extract combination p.o (each extract 500 mg/kg BW). Induction of ocular hypertension was performed by administering twelvedrops of 1% prednisolone acetate to the right eye. Intraocular pressure was measured using the schiotz tonometer before induction, after induction, and one hour after treatment. The combination of pomegranate and aloe vera extract given to rats with ocular hypertension showed a better potential to reduce intraocular pressure than a single administration of each extract, the percentage of reduction in intraocular pressure was 33.6±9.1%, 28.2±13.8%, and 29.9±8.1%, respectively. However, the combination of the two extracts did not show additive or synergistic effects and the potential of reducing intraocular pressure was lower than the acetazolamide drug. Keywords: Intraocular pressure, pomegranate, Aloe vera ABSTRAK Glaukoma merupakan penyebab kebutaan terbanyak ke dua di dunia. Strategi pengendalian tekanan intraokular untuk mencegah kebutaan akibat glaukoma dapat dilakukan dengan cara menghambat produksi dan atau melancarkan drainase cairan dalam bola mata. Salah satu sumber bahan baku alami yang potensial untuk pengendalian tersebut adalah buah delima (Punica granatum L.) dan lidah buaya (Aloe vera L.). Penelitian ini bertujuan untuk mengkaji potensi efek penurunan tekanan intraokular kombinasi buah delima dan lidah buaya pada tikus yang mengalami hipertensi okular. Buah delima dan lidah buaya diekstraksi dengan metode maserasi menggunakan etanol 70%. Secara acak, 30 ekor tikus dibagi kedalam 6 kelompok yaitu kelompok kontrol normal, kontrol tikus yang mengalami hipertensi okular, kontrol obat (asetazolamid 25 mg/kgBB p.o), ekstrak buah delima 500 mg/kgBB p.o, ekstrak lidah buaya 500 mg/kgBB p.o., kombinasi ekstrak p.o (masing – masing ekstrak 500 mg/kgBB). Induksi hipertensi okular dilakukan dengan pemberian 12 tetes prednisolon asetat 1% pada mata kanan. Tekanan intraokular diukur menggunakan tonomoter schiotz sebelum induksi, setelah induksi dan satu jam setelah pemberian perlakuan. Kombinasi ekstrak buah delima dan lidah buaya yang diberikan pada tikus yang mengalami hipertensi okular menunjukkan potensi yang lebih baik dalam menurunkan tekanan intraokular dibandingkan pemberian tunggal masing–masing ekstrak, persentase penurunan tekanan intra okular berturut-turut 33,6±9,1%, 28,2±13,8% dan 29,9±8,1%. Namun, pemberian kombinasi kedua ekstrak tersebut tidak menunjukkan efek aditif ataupun sinergis serta potensi dalam menurunkan tekanan intraokular masih dibawah obat asetazolamid. Kata kunci: Tekanan intraokular, buah delima, lidah buaya
Studies on biosurfactant produced using Exiguobacterium profundum Nur Asni Setiani; Welly Octaviyani; Syarif Hamdani; Irma Mardiah
Acta Biochimica Indonesiana Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i2.37

Abstract

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties. Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum. Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing. Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper. Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.
Identification of Endophytic Bacterial Isolated from Oil Palm Plants with Anti-Fungal Acitvity against Ganoderma boninense Irma Mardiah
Pharmacology and Clinical Pharmacy Research Vol 3, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (843.277 KB) | DOI: 10.15416/pcpr.v3i2.20005

Abstract

Endophytic bacteria is an excellent candidates for the biological control of pathogenic fungi in plantations. The objectives of this study were to isolate and examine anti-fungal activity of endophytic bacteria from oil palm plantations in South Kalimantan against Ganoderma boninense. This research was performed in three phases, i.e., isolation, selection, and assessment of endophytic bacteria isolates against Ganoderma boninense. A total of 126 colonies of endophytic bacteria were isolated. The result of the anti-fungal activity test with dual culture method demostrated that BKA 10 isolate had the largest inhibition zone (62.22%). Molecular identification by DNA analysis using 16S rRNA primers showed that BKA 10 is most closely related to Bacillus cereus. In conclusion, isolate with the best anti-fungal acitivity against Ganoderma boninense has the closest kinship with Bacillus cereus. Keywords: antifungal, dual culture, Bacillus cereus.
Utilization of Waste Frying Oil as A Source of Carbon in The Production of Biosurfactant using Exiguobacterium profundum Irma Mardiah; Kartika Puspitaningrum; Syarif Hamdani; Nur Asni Setiani
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2104

Abstract

Biosurfactant is a secondary metabolite produced by microorganisms that can be used as an alternative to environmentally friendly surfactants. Exiguobacterium profundum is one of the biosurfactants producers that potentially to be used in the pharmaceutical field. The use of waste frying oil as a carbon source can be used as a solution in overcoming the high cost of producing biosurfactants. The purpose of this study was to obtain optimum conditions in the production of biosurfactants by utilizing waste frying oil as a carbon source. In this study, variations in the optimized production conditions included the concentration of waste frying oil, labeled 2%, 3%, 4%, and 5%, and the medium pH at 6, 7, and 8. The study was using Mineral Salt Medium as production medium, the amount of inoculum concentration was 10% v/v, agitation speed 160 rpm, and incubation at room temperature. The optimum conditions for biosurfactant production were determined based on the best emulsification index. The biosurfactant extraction was carried out using a combination of chloroform and methanol (2:1) solvents. The best concentrations of waste frying oil for Exiguobacterium profundum was 5%, and the best medium pH was 7. Biosurfactants produced from Exiguobacterium profundum amounted to 8,2 g/L with an emulsification index 63,2%.
Computational Design of Nanobody Binding to Cortisol to Improve Their Binding Affinity Using Molecular Docking and Molecular Dynamics Simulations Umi Baroroh; Nur Asni Setiani; Irma Mardiah; Dewi Astriany; Muhammad Yusuf
Indonesian Journal of Chemistry Vol 22, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.71480

Abstract

Currently, nanobody binding cortisol has been deposited in the database. Unfortunately, the affinity is still in micromolar order. Substituting hydrophobic residues in the binding pocket and utilizing CDR2 and CDR3 is the strategy to improve the affinity. A single and double substitution at positions 53 and 101 have been introduced to the nanobody structure through molecular modeling. The affinity toward cortisol was evaluated using molecular docking to get the binding pose. The highest binding energy pose was used as the initial coordinate to analyze further using 100 ns molecular dynamics simulations. The binding affinities calculated by MMGBSA showed that MT3, MT5, and MT6 have better binding affinity than WT. In contrast, the ligand movement through MD simulations reveals that MT1, MT3, and MT5 are relatively stable. Hence, docking and MD simulations showed that MT3 is the best mutant than others. This mutant is substituting the threonine to isoleucine at position 53. New hydrophobic interactions occurred and caused the increase of binding. Eventually, this study provides valuable structural information to improve the binding affinity of nanobody binding cortisol for further development of this molecule to antibody-based biosensor design. 
Optimasi produksi biosurfaktan dari bakteri Bacillus cereus menggunakan minyak jelantah Irma Mardiah; Ika Fatimah; Nur Asni Setiani; Syarif Hamdani; Dewi Astriany
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p09

Abstract

Biosurfactant is a microorganisms metabolite product that have antibacterial activities. The purpose of this study was to obtain optimal conditions in the production of biosurfactants from Bacillus cereus by utilizing waste frying oil as a carbon source. In this study, variations in the optimized production conditions included the concentration of waste frying oil, labelled 2%, 3%, 4%, and 5% and the medium pH at 6, 7, and 8. The study was using Mineral Salt Medium as production medium, the amount of inoculum concentration was 10% v/v (108 CFU/ml), agitation speed 160 rpm and incubation at room temperature. The optimal conditions for biosurfactant production were determined based on the best emulsification index. The biosurfactant extraction was carried out using a combination of chloroform and methanol (2: 1) solvents. The best concentrations of waste frying oil was 3%, and the best medium pH was 6. Biosurfactants produced from Bacillus cereus bacteria was 8,9 g/L with an emulsification index value 57,17%.
Desain Peta Plasmid Pengkode α-Amilase Saccharomycopsis fibuligera R64 Mutan dan Pemodelan Struktur Protein Umi Baroroh; Shinta Kusumawardhani; Mia Tria Novianti; Muhammad Yusuf; Irma Mardiah; Ridha Nurfadlillah Azhari
Chimica et Natura Acta Vol 10, No 3 (2022)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v10.n3.42053

Abstract

Enzim α-amilase merupakan enzim yang banyak digunakan pada industri berbasis pati, diantaranya industri pangan, farmasi, tekstil, dan detergen. Enzim ini bahkan semakin dikembangkan di bidang biomedis, bioanalitik, dan kesehatan. α-Amilase dari Saccharomycopsis fibuligera R64 (Sfamy R64) merupakan enzim dari isolat lokal yang memiliki aktivitas amilolitik yang terbaik dari isolat lainnya, meskipun kemampuan adsorptivitasnya masih rendah. α-Amilase dengan adsorptivitas yang baik sangat diperlukan dalam industri pengolah pati karena dapat mengurangi penggunaan energi dan biaya produksi melalui penghilangan proses gelatinisasi. Dengan menambahkan sisi pengikatan di permukaan enzim melalui pergantian asam amino serin menjadi tirosin dan triptofan sehingga bersifat lebih aromatik telah dilakukan secara in silico dan menunjukkan hasil yang positif, mutan Sfamy R64 (sfamy R64 mut11). Protein ini dirancang pada sistem ekspresi Pichia pastoris secara ekstraselular dengan alfa-faktor sebagai sinyal peptida, promotor AOX1, dah penanda poli glisin dan histidine. Penelitian ini menggunakan berbagai web server online untuk merancang konstruksi gen dan pemodelan protein. Hasil perancangan didapatkan dengan mensubstitusi K48Q untuk menghindari pengenalan sisi Kex2 dan urutan nukleotidanya menunjukkan kandungan basa GC 38%, CAI 0,94, dan enzim restriksi yang digunakan adalah EcoR1 dan Not1. Hasil pemodelan struktur menunjukkan bahwa penanda poli glisin dan histidine terpapar keluar struktur dengan nilai DOPE sebesar -57260. Hasil perancangan ini diharapkan mampu mengekspresikan α-amilase rekombinan secara optimal.
PEMANFAATAN MINYAK JELANTAH UNTUK OPTIMASI PRODUKSI BIOSURFAKTAN DARI Brevundimonas terrae Irma Mardiah; Ika Fatimah; Syarif hamdani; Nur Asni Setiani
Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi Vol 22, No 1 (2022)
Publisher : LPPM Universitas Bakti Tunas Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36465/jkbth.v22i1.896

Abstract

Co-Authors Dewi Astriany Dewi Astriany Dewi Astriany Hamdani, Syarif Ika Fatimah Ika Fatimah Kartika Puspitaningrum Mia Tria Novianti MUHAMMAD YUSUF Muhammad Yusuf Nia Agustina Novi Irwan Fauzi Nur Asni Setiani Nur Asni Setiani Nur Asni Setiani Nur Asni Setiani Nur Asni Setiani Ridha Nurfadlillah Azhari Shinta Kusumawardhani Syarif hamdani Umi Baroroh Welly Octaviyani