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All Journal Berkala Kedokteran Universa Medicina Journal Of Vocational Health Studies
Fitranto Arjadi
Jenderal Soedirman University
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Paradoxical sleep deprivation changes testicular malondialdehyde and caspase-3 expression in male rats Fitranto Arjadi; Ginus Partadireja; Lientje Setyawan Maurits; Mulyono Pangestu
Universa Medicina Vol. 34 No. 2 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.87-95

Abstract

BACKGROUNDSleep deprivation is a significant problem among adult men and is considered as a risk factor for several diseases. Paradoxical sleep deprivation (PSD) induces Leydig cell apoptosis through elevation of corticosterone, with testicular malondialdehyde (MDA) and Leydig cell caspase-3 expression as parameters. The aim of this study was to observe testicular MDA level and caspase-3 expression treated with paradoxical sleep deprivation (PSD), immobilization, and footshock stress and to determine the stress model with a significant effect in white male rats (Rattus norvegicus) . METHODSThis experimental randomized study of posttest only with control group design was conducted on 24 white male Wistar strain rats, randomly allocated into four treatment groups, i.e. control (K1) without any stress treatment, PSD (KII), immobilization (KIII), and footshock stress (KIV). Treatments were given for 25 days to produce chronic stress. Testicular MDA concentration was examined by the ELISA method while caspase-3 was examined by the TUNEL method.RESULTSMean testicular MDA concentration with one-way ANOVA test showed differences in means between the groups (p=0.000) and post hoc Tukey-HSD test showed significant results between PSD stress group versus control, immobilization and footshock stress groups. One-way ANOVA test showed a significant difference in caspase-3 expression in at least two treatment groups (p=0.008) and post-hoc Tuckey-LSD test showed significant differences between controls and all stress groups. CONCLUSIONSleep deprivation is a type of stress inducing changes in testicular MDA concentration and caspase-3 expression in male rat testes.
EFFECT OF DIFFERENT TYPES OF SLEEP DEPRIVATION AND SLEEP RECOVERY ON SALIVARY PH Fani Tuti Handayani; Pratiwi Nur Widyaningsih; Fitranto Arjadi
Journal of Vocational Health Studies Vol. 4 No. 3 (2021): March 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V4.I3.2021.95-99

Abstract

Background: Salivary pH can rise or fall influenced by intrinsic and extrinsic factors. Sleep deprivation is one example of intrinsic factors. Sleep deprivation causes a reduction in sleep time at a certain time. Purpose: Analyze the effect of different types of sleep deprivations and sleep recovery on salivary pH. Method: This study was experimental research with a post-test only with a control group design. Thirty white Wistar strain rats were randomly divided into 5 groups: healthy control group (KI), partial sleep deprivation (PSD/KII), total sleep deprivation (TSD/KIII), partial sleep deprivation, and continued sleep recovery (PSD+SR/KIV) and total sleep deprivation and continued sleep recovery (TSD+SR/KV). The treatment is carried out on a single platform method. Salivary pH was measured with the help of color-coded pH strips that were given grading after the completion of sleep deprivation induction. Result: The mean decrease in salivary pH was highest in the TSD group. One Way ANOVA test showed significant differences (p <0.05) in the control group with PSD and TSD, the PSD group with PSD+SR, TSD group with PSD+SR and TSD+SR. Conclusion: Sleep deprivation is proven to reduce the pH of Saliva. Total sleep deprivation is a chronic condition that has the most influence on decreasing salivary pH. The effect of decreasing salivary pH due to sleep deprivation is proven to be overcome by sleep recovery.
Total Sleep Deprivation Reduces Quality of Spermatozoa in Male Albino Rat (Rattus norvegicus) Fitranto Arjadi; Sindhu Wisesa; Vitasari Indriani
Berkala Kedokteran Vol 19, No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v19i1.15719

Abstract

Abstract: Sleep deprivation-induced stress decreases the quality of spermatozoa due to the increase of glucocorticoid levels through the hypothalamic-pituitary-adrenal axis. Sleep recovery (SR) restores the effect of stress due to sleep deprivation by decreasing lipid peroxides and other free radicals and increasing glutathione antioxidants. This study aims to determine the spermatozoa quality in male albino rats (Rattus norvegicus) after the stress induction by diverse sleep deprivation protocols. This study is experimental research with posttest-only control group design in 30 male albino rats divided into five groups consisting of group I for negative control (no induction of sleep deprivation), group II for paradoxical sleep deprivation (PSD), group III for total sleep deprivation (TSD), group IV for PSD followed by SR, and group V for TSD followed by SR. PSD and TSD were performed by depriving rats sleep for 20 and 24 hours/day, respectively, for five days. SR was done for the next five days after PSD or TSD. Shapiro-Wilk test was used for normality of data distribution, and Levene's test was used for variance homogeneity. Comparative hypothesis was then analyzed with One Way Analysis of Variance (ANOVA) test, and significance between groups was analyzed with Tukey's Post-Hoc test. Group III (TSD) demonstrated the lowest mean of spermatozoa count (41,53±2,29), spermatozoa morphology (13,00±2,91), spermatozoa viability (31,00±7,39), and percentage of motility rate (37±8,58%) among all groups. In conclusion, total sleep deprivation (TSD) decreases the quality of spermatozoa in male albino rats (Rattus norvegicus).
Co-Authors Fani Tuti Handayani Ginus Partadireja Lientje Setyawan Maurits Mulyono Pangestu Pratiwi Nur Widyaningsih Sindhu Wisesa Vitasari Indriani