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All Journal Majalah Kedokteran Gigi Indonesia Jurnal Kesehatan Gigi Dental Journal (Majalah Kedokteran Gigi) Journal Of Vocational Health Studies Jurnal Material Kedokteran Gigi JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Mandala of Health : A Scientific Journal Medical and Health Journal

Pratiwi Nur Widyaningsih

Jurusan Kedokteran Gigi Fakultas Kedokteran Unversitas Jenderal Soedirman

Author-ID : 6126699

Humanities Dentistry Education Engineering Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Effect of various inductions of sleep deprivation stress on proinflammatory cytokine levels in gingival crevicular fluids of white male Wistar strain rats (Rattus novergicus) Pratiwi Nur Widyaningsih; Fitranto Arjadi; Erlina Sih Mahanani
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 1 (2020): March 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i1.p24-29

Background: Stress that is induced by sleep deprivation can modulate the damage of periodontal tissue by elevating the levels of proinflammatory cytokines (i.e. IL-1β and TNF-α). The effects of sleep deprivation can be resolved with sleep recovery. Gingival crevicular fluid (GCF) is fluid in sulcular gingiva which acts as an oral biomarker for evaluating periodontal abnormalities. Purpose: The aim of this study was to determine the effect of various induction methods of sleep deprivation stress on cytokine levels in GCF of white male Wistar strain rats (Rattus novergicus). Methods: The study method was true experimental with a posttest-only control group design. Thirty male Wistar rats were randomly divided into five groups: paradoxical sleep deprivation (PSD), total sleep deprivation (TSD), partial sleep deprivation with sleep recovery for five days (PSD+SR), total sleep deprivation with sleep recovery for five days (TSD+SR) and a healthy control group. Data were analysed via one-way ANOVA to determine differences between groups. Result: The results showed the highest level of IL-1β and TNF-α was found in the PSD group. One-way ANOVA analysis showed significant differences (p<0,05) of IL-1β level between PSD and control groups, PSD and PSD+SR groups and PSD and TSD+SR groups; in contrast, the analysis of TNF-α levels showed significant differences (p<0,05) between PSD group to control group, PSD to PSD+SR group and TSD to TSD+SR group. Conclusions: There is an effect of various induction methods of sleep deprivation stress on proinflammatory cytokines (IL-1β and TNF-α).

Effectiveness of capsaicin nanoparticle gel of Capsicum frutescens L. on oral squamous cell carcinoma in Rattus norvegicus Fitri Aniowati; Cantika Nadrotan Naim; Nova Dwi Anggraeni; Pratiwi Nur Widyaningsih
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 4 (2021): December 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i4.p210-215

Background: Oral squamous cell carcinoma (OSCC) is an oral cancer with a low life expectancy, less than five years after diagnosis. The drug therapy often used for OSCC patients is cisplatin, but it is considered to cause tumour persistence, drug resistance, and high toxicity. Therefore, it is important to test the development of alternative drugs from natural ingredients. One potential ingredient is green chilli pepper (Capsicum frutescens L.). It contains capsaicin that functions as an anticancer agent by suppressing BCa tumorigenesis so that proliferation is inhibited, as well as increasing and preventing p53 antibody mutations that play a role in cancer cell apoptosis. Purpose: This study aimed to compare effectiveness using capsaicin nanoparticle gel from green chilli pepper extract levels of 1% and 3.3% to reduce OSCC nodules. Methods: This study used 20 Rattus norvegicus that were randomly divided into five groups; C- (rat without treatment), C+ (rat induced to 7,12-Dimethylbenz(a)anthracene (DMBA)), E1 (DMBA exposed and given cisplatin, E2 (rat induced to DMBA and capsaicin extract nanoparticle gel with a concentration of 1%), and E3 (rat induced to DMBA and capsaicin extract nanoparticle gel with a concentration of 3.3%). The data were analysed statistically with the one-way ANOVA and least significance difference (LSD) test. Results: The comparison of mean nodule volume between C+ (5.834 ± 2.77 mm3) with E1 (1.75 ± 0.37 mm3), E2 (1.747 ± 0.36 mm3), and E3 (1.812 ± 0.11 mm3) had a significant difference (p = 0.00, p ≤ 0.05). Conclusion: Capsaicin nanoparticle gel with green chilli pepper extract at levels of 1% (E2) reduces OSCC nodules by more than gel with green chilli pepper extract at 3.3% (E3) concentration.

EFFECT OF DIFFERENT TYPES OF SLEEP DEPRIVATION AND SLEEP RECOVERY ON SALIVARY PH Fani Tuti Handayani; Pratiwi Nur Widyaningsih; Fitranto Arjadi
Journal of Vocational Health Studies Vol. 4 No. 3 (2021): March 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V4.I3.2021.95-99

Background: Salivary pH can rise or fall influenced by intrinsic and extrinsic factors. Sleep deprivation is one example of intrinsic factors. Sleep deprivation causes a reduction in sleep time at a certain time. Purpose: Analyze the effect of different types of sleep deprivations and sleep recovery on salivary pH. Method: This study was experimental research with a post-test only with a control group design. Thirty white Wistar strain rats were randomly divided into 5 groups: healthy control group (KI), partial sleep deprivation (PSD/KII), total sleep deprivation (TSD/KIII), partial sleep deprivation, and continued sleep recovery (PSD+SR/KIV) and total sleep deprivation and continued sleep recovery (TSD+SR/KV). The treatment is carried out on a single platform method. Salivary pH was measured with the help of color-coded pH strips that were given grading after the completion of sleep deprivation induction. Result: The mean decrease in salivary pH was highest in the TSD group. One Way ANOVA test showed significant differences (p <0.05) in the control group with PSD and TSD, the PSD group with PSD+SR, TSD group with PSD+SR and TSD+SR. Conclusion: Sleep deprivation is proven to reduce the pH of Saliva. Total sleep deprivation is a chronic condition that has the most influence on decreasing salivary pH. The effect of decreasing salivary pH due to sleep deprivation is proven to be overcome by sleep recovery.

Peningkatan Peran Guru Sekolah Dasar Dalam Pengobatan Darurat untuk Menghilangkan Rasa Sakit Gigi dan Mulut Ryana Budi Purnama; Helmi Hirawan; Tirta Wardana; Mutia Rochmawati; Pratiwi Nur Widyaningsih; Dian Noviyanti; Desi Rachmawati; Rakhmawati Rakhmawati; Shahnaz Dwi Permata Putri; Minda Anita; Kunthi Isri Wulandari; Dani Intan Prabawati
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 5 No 1 (2021): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Perkumpulan Dosen Indonesia Semesta (DIS) Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v5i1.341

Abstract - Oral and dental health is a condition that needs to be considered and maintained to improve the quality of life. One of the efforts made to maintain dental and oral health in the school environment is by conducting UKGS (Usaha Kesehatan Gigi Sekolah). In UKGS activities, teachers have a role in providing emergency treatment to relieve pain. The emergency treatment administered by teachers in schools can perform in cases such as avulsions, choking, and abscesses. This community service aims to increase the role of elementary school teachers through UKGS to maintain, improve the oral health of all students at SDN in the Karangtengah area, Baturaden District, Purwokerto. The method of implementation was basely on optimizing the potential of UKGS teacher cadres, including identification of target schools and participants, pre-test implementation, maturation, coaching, enhancement of UKGS teacher cadres. Coaching and training have been carried out for UKGS supervisors for abscess, choking and avelse conditions. Post-test activities to gain insight into emergency medicine and relieve tooth and mouth pain. Results: p data analysis using the Wilcoxon asymp test.sig.(2-tailed) of 0.001 (<0.05), this value indicates that there is an influence on the level of knowledge about emergency medicine. The analysis performed with the Wilcoxon Test; there was a significant difference (sig = 0.001, p <0.05). There was an increase in the average pre-test value of 76.92 and post-test of 97.85.Conclusion: There is increasing knowledge about emergency medicine to relieve tooth and mouth pain in elementary school teachers.

Perbedaan Kebocoran Mikro komposit Nanohybrid Pasca Aplikasi Etsa Asam Tartarat Antara Konsentrasi 10%, 35%, dan 40 Luqman Mahadika Cahyono; Irfan Dwiandhono; Ryana Budi Purnama; Rinawati Satrio; Pratiwi Nur Widyaningsih
Jurnal Material Kedokteran Gigi Vol 10 No 2 (2021): JMKG Vol 10 No 2 September 2021
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v10i2.960

Composite resin is the material most often used as a restorative material. Acid etching is one of the procedures to help provide a place for penetration of bonding materials and composite resins. The acidic etching material that is often used is 37% phosphoric acid, but 37% phosphoric acid can cause inflammatory cell infiltration. Tartaric acid composed of weak acids that has potential to be an alternative acid etching. The aim of this study is to determine whether there are differences in microleakage of nanohybrid composites after application of tartaric acid etching between concentrations of 10%, 35%, and 40% in prevention of microleakage. This research is a laboratory experimental research with a posttest-only group design. Research samples are 24 maxillary first premolar were divided in 4 groups. Group I with 10% tartaric acid, group II with 35% tartaric acid, group III with 40% tartaric acid and group IV with 37% phosphoric acid etching. Based on the results of the LSD test it shows that There is microleakage of nanohybrid composites after etching application of tartaric acid concentrations of 10%, 35%, 40%, and 37% phosphate in class I cavities. Tartaric acid concentration of 40% can be used as an alternative to etching 37% phosphoric acid in preventing microleakage.

PREDICTION OF THE ROLE OF CURCUMINOIDS IN CURCUMA LONGA AS GSK-3 INHIBITORS ON TERTIER DENTIN GROWTH Naila Robbaniyya Fithriyya; Hanifah Nur Syafa; Dwi Cinthya Financia; Nadya Elsa Cahyaningrum; Husni Rama Wijayanti; Haidy Lailatun Nabila; Salma Kaltsum Kayana; Ghaida Humaira Susilo Putri; Nurul Hasanah Tanjung; Pratiwi Nur Widyaningsih; Ryana Budi Purnama; Tirta Wardhana
Medical and Health Journal Vol 1 No 1 (2021): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Caries is one of the most common oral diseases. Tertiary dentin regeneration has recently been developed as an alternative treatment for caries. Curcuminoids are known to have the ability to inhibit GSK-3β in the Wnt signalling pathway to accelerate the formation of reparative dentin. This study aimed to investigate curcuminoids as GSK-3β inhibitors in modulating tertiary dentin formation through the Wnt pathway. Insilico analysis has been carried out using an online database and software. Data collection is done through the Protein Data Bank https://www.rcsb.org/ dan PubChem https://pubchem.ncbi.nlm.here.gov/. The analysis of interactions between proteins and bioactive compounds through the STITCH website http://stitch.embl.de/ dan STRING https://string-db.org/.The results obtained that STK3 protein that binds to curcuminoids has a hippo signalling pathway that inhibits GSK-3β in the Wnt pathway. The study concludes that curcuminoids have the potential to accelerate the development of tertiary dentin.

POTENSI PROPOLIS TERADAP PENINGKATAN KADAR FIBRINOGEN PADA PROSES PENYEMBUHAN LUKA PASCA PENCABUTAN GIGI Nisa Aqila Zulfa; Nur Faizah Zahirah; Arina Manasikana Eska; Nadya Loverina G.P; Nabilah Annisa R; Artha Arum P; Haryani Devi R; Elisabeth Lorna Dona; Ayu Intan Nur A; Pratiwi Nur Widyaningsih; Ryana Budi Purnama; Tirta Wardana
Medical and Health Journal Vol 1 No 1 (2021): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Proses pencabutan gigi dilakukan untuk mengeluarkan gigi yang utuh atau sisa akar tanpa meninggalkan trauma rasa sakit. Pencabutan gigi termasuk dalam tindakan bedah minor yang melibatkan jaringan keras gigi. Penyembuhan luka sangat erat kaitaya dengan peran thrombin untuk mengubah fibrinogen menjadi fibrin untuk menghasilkan pembekuan darah sehingga menyebabkan pendarahan berhenti. Namun, Proses penyembuhan luka soket pasca pencabutan normalnya membutuhkan waktu yang lama sehingga dibutuhkan satu alternative terapi untuk mempercepat penyembuhan luka. Propolis dianggap sebagai substansi resin lebah yang mengandung berbagai senyawa organik berperan untuk meningkatkan penyembuhan luka melalui perubahan fibrinogen menjadi fibrin. Penelitian dilakukan sedangan studi in silico untuk mempelajar kandungan senyawa propolis yang dapat memengaruhi peningkatan pembentukan fibrin untuk mempercepat penyembuhan luka. Crystal struktur dari human fibrinogen didapatkan dari http://rscb.org/. senyawa asam sinamat sebagai agen koagulan dar propolis dilakukan analisis menggunakan http://pubchem.ncbi.nlm.nih.gov/. analisis mekanisme hubungan interaksi senyawa dengan protein yang berperan proses penyembuhan luka menggunakan http://stitch.embl.de/. pada penelitian ini kami menemukan 11 protein yang dipengaruhi ekspresinya oleh senyawa asam sinamat (Cinnamic acid). Protein F2, F9, SERPIND, SERPIND1, CDK8, MED1, MED6, MED7, MED10, MED 12, MED14, MED18, MED21. Analisis fungsi menujukan bahwa protein F2 dalam asam sinamat mengaktifkan reseptor trombin yang kemudian mengubah fibrinogen menjadi fibrin. Kesimpulan pada penelitian ini diketahui bahwa kandungan senyawa asam sinamat pada propolis dapat memengaruhi aktivasi reseptor thrombin untuk mempercepat penyembuhan luka.

POTENTIAL COMPOUND EXTRACT FROM POTENTIAL COMPOUND EXTRACT FROM Carica pubescens ON GINGIVA INCISION WOUND HEALING: Through Proliferation, Differentiation and Immunoresponse Mechanisms Salwa Az-Zahra; Sabrina Aulia Nurul Hidayah; Septina Nur Aini; Aprillya Yuniarni; Annisa Sukma Visanda; Asyyfa Devy; Amalia Putri Hesantera; Raja Ronaldo Siregar; Dicky Satria Ramadhan Basar; Pratiwi Nur Widyaningsih; Ryana Budi Purnama; Tirta Wardana
Medical and Health Journal Vol 1 No 1 (2021): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Luka menyebabkan gangguan struktur normal, sehingga penyembuhan luka dibutuhkan untuk perbaikan struktur sel dan jaringan. Beberapa sitokin dan growth factor yang memiliki peran penting pada proses penyembuhan luka diantaranya PDGF, FGF, TGF-β, VEGF, Angiopoetin, IL-1, IL-6, IL-10, TNF-α, IFN-γ, makrofag yang diproduksi oleh limfosit dan leukosit pada tahap sintetis kolagen. Daun papaya (Carica pubescens) diketahui memiliki kandungan flavonoid terdiri seperti apigenin, chyrsin, diosmetin, kaempferol, luteolin, naringenin dan quercetin yang berperan dalam meninkatkan ekspresi IL-10 untuk penyembuhan luka. Penelitian ini bertujuan untuk mengetahui kandungan senyawa dari Senyawa flavonoid Carica pubescens untuk meningkatkan kinerja IL-10. Penelitian ini dilakukan dengan menggunakan pendekatan bioinformatika menggunakan software web online server. Pencarian senyawa potensial didapatkan dari https://pubmed.ncbi.nlm.nih.gov/, senyawa kimia menggunakan http://www.pubchem.ncbi.nlm.nih.gov/ dengan struktur 3D. analisis prediksi bioaktivitas menggunakan http://stitch.embl.de/ dengan nilai indicator lebih dari 0.7 (Pa>0.7). Analisis ikatan kimia menggunakan http://stitch.embl.de/ dan http://string-db.org/. Analisis secara in silico menunjukkan bahwa senyawa kaemfrerol memiliki potensi meningkatkan kadar IL-10. Ditambah lagi, kami menemukan beberapa kandungan senyawa lainya yang berhubungan dengan penyembuhan luka seperti rutin, peroxynitrite, iloprost, quercetin. Pada penelitian ini, kami menyimpulkan bahwa senyawa kaempferol memiliki potensi sebagai alternative dalam pengembangan terapi untuk mempercepat penyembuhan luka.

POTENSI SENYAWA RUTIN DARI JERUK POTENSI SENYAWA RUTIN DARI JERUK NIPIS (Citrus aurantifolia) TERHADAP KADAR TNF-α UNTUK TERAPI PERIODONTITIS KRONIS Aldina G Aldina G; Setyah Sultrasari D; Shabrina Nur Zahra S; Adhela Salsabila H; Licha Permata S; Yunita K Yunita K; Nadya Hanan S; Diva Sascha P; Ridha Nur A; Pratiwi Nur Widyaningsih; Ryana Budi Purnama; Tirta Wardana
Medical and Health Journal Vol 1 No 1 (2021): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Periodontitis merupakan suatu penyakit infeksi pada jaringan pendukung gigi yang disebabkan oleh mikroorganisme dan terjadi kerusakan progresif pada jaringan periodontal. Periodontitis didominasi oleh bakteri Porphyromonas gingivalis, bakteri ini dapat merangsang sitokin proinflamasi salah satunya TNF-α yang merupakan bagian pathogenesis infeksi melalui mekanisme sel-sel fagosit yang akan berperan pada penyakit periodontitis. Perawatan periodontitis dapat dilakukan dengan scaling root planning dan pemberian obat obatan herbal salah satunya adalah jeruk nipis, tanaman ini bermanfaat sebagai antiinflamasi, antipiretik dan antibakteri. Jeruk nipis memiliki kandungan senyawa saponin dan flavonoid yaitu hesperidin, tangeretin, naringin, ericocitrin, eriocitroid. Tujuan: Tujuan dari penelitian ini adalah mengetahui hubungan flavonoid terhadap sintesis TNF-α pada periodontitis kronis yang dianalisis secara bioinformatika melalui tiga website antara lain pubchem, stitch, dan string. Metode: Metode yang digunakan dalam penelitian ini adalah metode in silico. Hasil dan kesimpulan: Kandungan flavonoid pada kulit jeruk nipis terbukti memiliki hubungan terhadap sintesis TNF-α yaitu rutin. Rutin termasuk kedalam kelompok flavonol yang terdapat pada gugus di posisi 3 pada cincin C. Gugus aromatik cincin B merupakan gugus yang bertanggung jawab atas aktivitas flavonol karena ikatan rangkap konjugasi pada nomor 2′ dan 3′ memiliki kemampuan untuk perpindahan elektron dari cincin B menuju radikal bebas dan memecah radikal bebas.

PENGARUH PEMBERIAN EKSTRAK CABAI RAWIT (Capsicum frustescens L.) TERHADAP GAMBARAN HISTOLOGI ORAL SQUAMOUS CELL CARCINOMA (STUDI IN VIVO) Pratiwi Nur Widyaningsih; Fitri Aniowati; Cantika Nadrotan Naim; Nova Dwi Anggraeni
Mandala Of Health Vol 13 No 2 (2021): Mandala Of Health
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2021.13.2.8906

Oral squamous cell carcinoma (OSCC) is a cancer of the oral cavity that is often associated with a low life expectancy. OSCC is a multifactorial disease, and its symptoms typically manifest as ulceration with exophytic and persistent edges. Cisplatin is a commonly used chemotherapeutic agent for treating OSCC, but it has high toxicity. Cayenne pepper (Capsicum frutescens L.) is a widely consumed plant that contains capsaicin, which can act as an anticancer agent by increasing p53 antibodies in the apoptosis process, thereby preventing cancer cell proliferation and degrading p53 gene mutations. This study aims to determine the effect of administering cayenne pepper extract (Capsicum frutescens L.) on the histological features of oral squamous cell carcinoma (in vivo study). Thirty male Wistar rats, aged 2 months and weighing 150-200 grams, were divided into five groups: negative control group (K-) without treatment, positive control group (K+) given DMBA exposure, treatment group with DMBA exposure and cisplatin induction, treatment group with DMBA exposure and induction of gel nanoparticles containing 1% capsaicin extract, and treatment group with DMBA exposure and induction of gel nanoparticles containing 3% capsaicin extract. The data were analyzed statistically using the One-Way ANOVA test followed by Post hoc LSD. The results showed significant differences (P<0.05) among all groups, except between KP1 and KP2, KP3 and K+, and KP1 and KP-. The administration of 1% capsaisen nanoparticle gel had a similar effect to cisplatin administration in terms of the number of mitoses and atypical cells under OSCC conditions.

Co-Authors Adhela Salsabila H Aldina G Aldina G Amalia Putri Hesantera Annisa Sukma Visanda Aprillya Yuniarni Arina Manasikana Eska Aris Aji Kurniawan Artha Arum P Asyyfa Devy Ayu Intan Nur A Cantika Nadrotan Naim Cantika Nadrotan Naim Dani Intan Prabawati Desi Rachmawati Dian Noviyanti Dicky Satria Ramadhan Basar Diva Sascha P Dwi Cinthya Financia Elisabeth Lorna Dona Erlina Sih Mahanani, Erlina Sih Fani Tuti Handayani Fitranto Arjadi Fitranto Arjadi Fitri Aniowati Fitri Aniowati Ghaida Humaira Susilo Putri Haidy Lailatun Nabila Hanifah Nur Syafa Haryani Devi R Helmi Hirawan Husni Rama Wijayanti Irfan Dwiandhono Irfan Dwiandhono Kunthi Isri Wulandari Licha Permata S Luqman Mahadika Cahyono Minda Anita Mutia Rochmawati Nabilah Annisa R Nadya Elsa Cahyaningrum Nadya Hanan S Nadya Loverina G.P Naila Robbaniyya Fithriyya Nisa Aqila Zulfa Nova Dwi Anggraeni Nova Dwi Anggraeni Nur Faizah Zahirah Nurul Hasanah Tanjung Raihan Zachary Ramadhan Raja Ronaldo Siregar Rakhmawati Rakhmawati Rakhmawati Rakhmawati Ridha Nur A Rinawati Satrio Riski Amalia Hidayah Ryana Budi Purnama Ryana Budi Purnama Ryana Budi Purnama Sabrina Aulia Nurul Hidayah Salma Kaltsum Kayana Salwa Az-Zahra Septina Nur Aini Setyah Sultrasari D Shabrina Nur Zahra S Shahnaz Dwi Permata Putri Tirta Wardana Tirta Wardana Tirta Wardana Tirta Wardhana Yunita K Yunita K

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