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All Journal Meditory : The Journal of Medical Laboratory Jurnal Dunia Farmasi Borneo Journal of Medical Laboratory Technology Prosiding Seminar Nasional Hasil-hasil Penelitian dan Pengabdian Pada Masyarakat
Kurniawan Kurniawan
Universitas Muhammadiyah Purwokerto
Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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Analisis Kadar Rhodamin B pada Terasi yang Diperjualbelikan di Pasar Belik Kabupaten Pemalang Oden Krisyan; Retno Sulistiyowati; Kurniawan Kurniawan
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2143

Abstract

Terasi is a cooking spice made from fermented shrimp and is often found in the market. It is relatively affordable and easy to find, so people flavor it as a flavor enhancer for their food. The abuse of Rhodamine B as a Food Additive (BTP) in terasi is often done by the producers. This study aimed to analyze the Rhodamine B content in the terasi sold at the Pasar Belik, Pemalang Regency. The study was conducted in a descriptive observational manner where the sample was analyzed qualitatively, and if the result were positive, it would be followed by quantitative. The study was conducted in December 2020. Rhodamine B content in terasi was analyzed by univariate test. Qualitative analysis was carried out by chemical methods using 10% NaOH, Petroleum Ether, and 10% HCl. The qualitative analysis of 7 samples of shrimp paste sold at the Belik Market in Pemalang Regency were all negative and did not contain Rhodamine B. Shrimp samples that were bought and sold at the Pasar Belik in Pemalang Regency showed that they were free from Rhodamine B.
Pengaruh Variasi Suhu dan Lama Penyimpanan terhadap Viabilitas Bakteri Asam Laktat (BAL) dan Nilai pH Yoghurt Aditya Dimas Pangestu; Kurniawan Kurniawan; Supriyadi Supriyadi
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2169

Abstract

Consuming yogurt indicates the increasing public awareness of healthy lifestyles has led many people to consume yogurt. Yogurt is a fermented milk product by adding pasteurized skim milk concentrate and adding lactic acid bacteria culture. L. casei is the bacterial culture commonly used in the production of yogurt, which has a role in breaking down lactose into lactic acid. To maintain its quality, yogurt is generally stored at a low temperature. However, there are still many business actors who only store it in a cool box or even at room temperature. The purpose of this study was to describe the viability of LAB and the effect of variations in temperature and storage time on the viability of LAB and pH value of yogurt. This study used an experimental method with a completely randomized design (CRD) with 3 treatments in the form of variations in storage temperature, namely 5°C, 15°C, and 25°C, each treatment temperature was stored for 1 week, 2 weeks, 3 weeks, and 4 weeks. This study was conducted in December 2020. The results showed that the highest viability value (289x106 CFU/mL) of lactic acid bacteria in yogurt was obtained at 5°C with two weeks of storage. Yogurt's pH value is influenced by temperature and storage time; the higher the temperature and storage time, the causes the yogurt's pH value decreases. The conclusion that can be drawn from this study is that the highest viability of LAB was obtained at 5°C with a shelf life of two weeks, while the best pH value of yogurt was achieved at 5°C for one week.
EFEKTIVITAS EKSTRAK KAPANG ENDOFIT ISOLAT BR-S1 (A) TERHADAP BAKTERI Methicillin-Resistant Staphylococcus aureus (MRSA) Kurniawan kurniawan kurniawan; Nuniek Ina Ratnaningtyas
Meditory : The Journal of Medical Laboratory Vol 6, No 2 (2018): Meditory, volume 6, nomer 2, Desember 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.83 KB) | DOI: 10.33992/m.v6i2.417

Abstract

Background Tea parasitic plant (Scurrula oortiana L.) is one of medicinal plants (herba medicina) containing several active compounds but its utilization is still constrained by some problems, so that there needs other approaches by utilizing potential of BR-S1 (A) isolate endophytic fungi which grows in these plants to control MRSA pathogenic bacteria. Objective This research aimed to examine the effectiveness of the four types of BR-S1 (A) endophytic fungi extract  from the origin of tea parasites to the growth of MRSA bacteria.Methods The researchers used laboratory experimental method by Kirby Bauer disk diffusion method and the data obtained were analyzed statistically. Result The research result showed that MRSA bacteria had various sensitivity on four types of BR-S1 (A) isolate endophytic fungi; with its highest sensitivity on extract of ethyl acetate and its lowest sensitivity was extract of n-hexane (no inhibition). The difference of sensitivity test results was suspected to be related to active compound content found in ethyl acetate extract in form of flavonoid and tannin class compounds which had been proven to inhibit growth of pathogenic bacteria.  Conclusion It can be concluded that  the four types of BR-S1 (A) endophytic fungus extract originating from tea parasites, only ethyl acetate extract was the most effective in inhibiting the growth of MRSA bacteria, this is due to the content of two active compounds from the flavonoid and tannin groups.
PENINGKATAN KEWASPADAAN DALAM PENCEGAHAN KEJADIAN DEMAM BERDARAH DENGUE DAN TOKSOPLASMOSIS PADA ANGGOTA RANTING AISYIYAH KARANG PUCUNG KECAMATAN PURWOKERTO SELATAN Dita Pratiwi Kusuma Wardani; Kurniawan Kurniawan
PROSIDING SEMINAR NASIONAL LPPM UMP Vol 1 (2019): PROSIDING SEMINAR NASIONAL LPPM UMP 2019
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kejadian demam berdarah dengue masih menjadi perhatian tidak hanya bagi pemerintah, namun menjadi perhatian masyarakat Indonesia. Demam berdarah dengue yang tidak segera mendapat penanganan dapat mengakibatkan kematian sedangkan prevalensi dari tahun ke tahun selalu mengalami kenaikan yang signifikan. Padahal sering dilakukan Pemberantasan Sarang Nyamuk (PSN) secara berkala. Begitu pula dengan kasus tokspolasmosis yang menyerang wanita usia subur sehingga perlu menjadi perhatian karena toksoplasmosisdapat menyebabkan keguguran bagi wanita hamil bahkan menyebabkan kecacatan pada janin. Ranting Aisyiyah Karang Pucung Kecamatan Purwokerto Selatan merupakan kumpulan ibu-ibu yang aktif melakukan kegiatan pengajian dan diskusi. Belum pernah dilakukan penyuluhan mengenai kewaspadaan pencegahan kejadian demam berdarah dengue dan toksoplasmosis sehingga timbul inisiatif untuk memberikan edukasi mengenai pencegahan kejadian demam berdarah dan toksoplasmosis. Kegiatan ini diharapkan dapat memberikan kontribusi positif anggota Aisyiah berperan penting dalam kepedulian terhadap lingkungan sekitar terutamadalam hal pencegahan kejadian demam berdarah dan toksoplasmosis.
Analisis GCMS dan Formulasi Gel Arang Tempurung Kelapa sebagai Kandidat Obat bagi Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA) Kurniawan Kurniawan
Jurnal Dunia Farmasi Vol 7, No 3 (2023): Edisi Agustus
Publisher : Program Studi Farmasi, Fakultas Farmasi dan Kesehatan, Institut Kesehatan Helvetia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33085/jdf.v7i3.5787

Abstract

Pendahuluan: Bakteri Methicillin-resistant Staphylococcus aureus (MRSA) merupakan bakteri S. aureus yang telah mengalami resistensi terhadap antibiotik β-laktam. Munculnya bakteri MRSA membuat pengobatan menjadi semakin sulit sehingga perlu adanya upaya pencarian senyawa aktif dari bahan alam, salah satunya adalah arang tempurung kelapa yang telah terbukti memiliki manfaat di bidang kesehatan. Tujuan: mengetahui jenis-jenis senyawa aktif yang terkandung pada ekstrak arang tempurung kelapa; mengetahui jenis-jenis senyawa aktif yang terkandung pada ekstrak arang tempurung kelapa yang berpotensi sebagai senyawa antibakteri dan mendapatkan formulasi gel arang tempurung kelapa yang optimal sebagai kandidat obat bagi bakteri MRSA. Metode: Penelitian ini termasuk penelitian eksperimental yang dilaksanakan secara blended learning (online/daring). Hasil: Dari penelitian ini diperoleh hasil penelitian berupa ekstrak arang tempurung kelapa sebanyak 11,23 g, 731 senyawa aktif dengan 3 senyawa dominan yaitu alpha-Santalol (CAS), Eicosane (CAS) dan Santalol (CAS). Hasil formulasi diperoleh 3 konsentrasi gel yaitu 3%, 6% dan 9% dengan hasil uji stabilitas yang baik. Kesimpulan: Terdapat 731 jenis senyawa aktif yang terkandung di dalam ekstrak arang tempurung kelapa dengan 3 senyawa aktif utama (dominan) yaitu alpha.-Santalol (CAS), Eicosane (CAS) dan Santalol (CAS). Eicosane (CAS) merupakan senyawa aktif pada ekstrak arang tempurung kelapa yang memiliki potensi sebagai senyawa antibakteri. Formulasi gel arang tempurung kelapa 9% paling optimal sebagai kandidat obat bagi bakteri MRSA.
Co-Authors Aditya Dimas Pangestu Dita Pratiwi Kusuma Wardani Nuniek Ina Ratnaningtyas Oden Krisyan Retno Sulistiyowati Supriyadi Supriyadi