Heri Wijaya
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PENETAPAN KADAR FLAVONOID EKSTRAK TERPURIFIKASI UMBI BAWANG DAYAK (Eleutherine palmifolia (L). Merr) SECARA SPEKTROFOTOMETRI Siti Jubaidah; Eka Siswanto; Heri Wijaya; Muhammad Putu Aditya
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 4 No 1 (2019): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.018 KB) | DOI: 10.36387/jiis.v4i1.225

Abstract

Eleutherine palmifolia (L) Merr. is one of the plant that has function as medicine. One of the compound which much contained in ethanol extract of dayak union is flavonoid, so that could separate with purification. The aim of this study is to determine flavonoid levels in purified extract of dayak union root with various drying treatments using UV-Vis spectrophotometry and to find out which drying treatment that obtaine the highest flavonoid levels.Drying treatment of dayak union root simplicia were with direct sunlight for 6 hours (P1), dried by oven with temperature 60ºC for 6 hours (P2), dried by covered with black clothes (P3). Dayak union root simplicia extracted by maceratiin method using ethanol 70%, and purified step by step using n-hexane solvent. Flavonoid levels test of purified extract of dayak union root carried outby UV-Vis spectrophotometry method.The results of UV-Vis spectrophotometry obtained flavonoid levels of purified extract of dayak union root in dried by direct sunlight (P1) was 0.79%, dried by oven with temperature 60ºC (P2) was 1.57%, and dried by coveredwith black clothes (P3) was 1.02%. LSD test shows (P<0.05) the significant differences between flavonoid levels with different drying treatment.
Pengaruh Logam Berat Kadmium (Cd) Terhadap Ekspresi GEN cGnRH-II Pada Ikan Nilem (Osteochilus hasselti C.V) Jantan Norman Arie Prayogo; Asrul Siregar; Heri Wijaya; Purnama Sukardi; Ren Fitriadi
Jurnal Akuakultur Sungai dan Danau Vol 8, No 2 (2023): Oktober
Publisher : Universitas Batangahari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/akuakultur.v8i2.168

Abstract

Logam berat kadmium di perairan pada umumnya mempunyai sifat toksik dan berbahaya bagi organisme hidup, walaupun beberapa diantaranya diperlukan dalam jumlah kecil. Cd merupakan logam berat yang sangat berbahaya karena tidak dapat dihancurkan (non-degradable) oleh organisme hidup dan dapat terakumulasi ke tubuh organisme melalui rantai makanan. Tujuan dari penelitian ini adalah mengetahui pengaruh Cd pada reproduksi ikan nilem (Osteochilus hasselti C.V) jantan. Prosedurnya hewan uji disimpan di empat bak fiber yang mengandung Cd dengan kadar (0 mg/L [kontrol]; 2 mg/L [rendah]; 4 mg/L [sedang]; 6 mg/L [tinggi]) selama 4 minggu. Dampak dari Cd terhadap reproduksi ikan nilem dievaluasi dengan tingkat ekspresi gen cGnRH-II. Hasil penelitian menunjukkan bahwa pengaruh perlakuan kadmium selama 4 minggu terhadap nilai ekspresi gen cGnRH-II memiliki kisaran rata-rata antara 0,0036 - 0,3470. Pengaruh perlakuan selama minggu ke-2 dan ke-4 tidak memberikan pengaruh yang signifikan (P>0,05) dalam menurunkan nilai ekspresi gen cGnRH-II pada ikan nilem jantan, seiring dengan meningkatnya konsentrasi kadmium serta lamanya waktu pemaparan.