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All Journal Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan
Wihasty Nur Istyqomah
Fakultas Farmasi, Universitas Ahmad Dahlan Yogyakarta
Medicine & Pharmacology

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VALIDASI METODE KROMATOGRAFI LAPIS TIPIS (KLT)-DENSITOMETRI UNTUK PENETAPAN KADAR β-KAROTEN DALAM TABLET KUNYAH EKSTRAK Spirulina platensis Siti Fatmawati Fatimah; Citra Ariani Edityaningrum; Wihasty Nur Istyqomah; Ibnu Gholib Gandjar; Laela Hayu Nurani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 1 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.6 KB) | DOI: 10.36387/jiis.v5i1.404

Abstract

Analytical Validation Method to determine β-carotene in the Spirulina platensis extract chewable tablets need to be done to ensure the quality. The purpose of this study is to prove the validity of the TLC-Densitometry method for determining the levels of β-carotene in the Spirulina platensis extract chewable tablets. Validation of the analysis method was carried out using standard β-carotene and spirulina chewable tablets in ethyl acetate solvents including specificity, linearity, repeatability precision, intermediate precision, limit of detection and limit of quantitation with stationary phase of silica gel 60 F254 and mobile phase of acetone: ethyl acetate (1: 1 v/v) using the TLC method, then quantitative analysis was carried out with a Densitometer at a wavelength of 453 nm. The results showed that the TLC-Densitometry method was linear with an r value of = 0.9938, having a repeatability precision with RSD 1.89%, intermediate precision with an RSD value of 2.401%, an LOD of 0.159 mg/mL and an LOQ of 0.531 mg/mL, and the specificity showed that this method is not specific to β-carotene compounds and the accuracy does not indicate a recovery value. Based on these results it can be concluded that the TLC-densitometry method is invalid for the determination of β-carotene levels in the Spirulina platensis extract chewable tablets.
Co-Authors Citra Ariani Edityaningrum Ibnu Gholib Gandjar Laela Hayu Nurani Siti Fatmawati Fatimah, Siti Fatmawati