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D. Purwantini
Faculty of Animal Science, University of Jenderal Soedirman, Jl. Dr. Soeparno No. 60, Purwokerto 53122
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Morphometrics and genetic diversity of Tegal, Magelang and their crossbred ducks based on Cytochrome b gene Henrik, H.; Purwantini, D.; Ismoyowati, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 1 (2018): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.1.9-18

Abstract

This experiment was conducted to study the morphometric and genetic diversity of Tegal, Magelang, and their crossbred ducks. Each ten female about twenty weeks old ducks from Tegal, Magelang, Gallang, and Maggal ducks, respectively, were used as the group materialss. The body weight, chest circumference, body length, shank length, neck length, and pubis width were recorded and tested by analysis of variance of one way classification as the morphometric characteristics. Polymorphism of cytochrome b (cyt b) gene on mitochondrial DNA (mtDNA) was analyzed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Genetic distance was analyzed based on value of heterozygosity, whereas the phylogeny tree was reconstructed using MEGA6 software. The results showed there were highly significant difference (P<0.01) on body weight, chest circumference, body length, and neck length between population, while shank and pubis width were not significant different. The genetic distance between Gallang and Maggal ducks (0.206) was higher than Tegal and Magelang ducks (0.169). It is concluded that the reciprocal crosses increased the morphometric and genetic diversity of Indonesian local duck population.
ESTIMATION OF SELECTION ACCURACY AND RESPONSES OF THE PRODUCTION CHARACTERISTICS USING DIFFERENT SELECTION INTENSITY IN MAGELANG DUCK Purwantini, D.; Ismoyowati, I.; Santoso, S. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 41, No 2 (2016): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.41.2.61-69

Abstract

This research was aimed to estimate selection response and accuracy of hatching weight, growth and egg production using different selection intensities in Magelang duck. A nested design was used in this study with experimental material was Magelang duck consisted of 8 male (treatments), 40 female (sub-treatments) and 360 offspring (replicates) and the observed parameter was productive characteristics. Nine DOD from each female were measured for hatching weight and growth up to 8 weeks old. The measured Hen Day Production (HDP) at initial laying for within days. Result showed that different selection intensities (25, 50 and 75%) led to response in 2.968; 1.870 and 0.982 g hatching weight; respectively; 0.00221, 0.00139 and 0.00073 g growth, respectively; and 1.728, 1.088 and 0.571% egg production, respectively. Selection accuracy for hatching weight, growth and egg production was 0.70, 0.76 and 0.51, respectively. Conclusively, the less preserved female proportion, the higher selection intensity value thus the higher selection response. Selection accuracy of production characteristics was based on its heritability value.
MORPHOLOGY AND GENETIC DIVERSITY OF MITOCHONDRIAL DNA D-LOOP REGION USING PCR-RFLP ANALYSIS IN MAGELANG DUCK AND OTHER NATIVE DUCK Purwantini, D.; Yuwanta, T.; Hartatik, T.; Ismoyowati, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.1-9

Abstract

The aim of this study was to investigate the different of plumage colors on morphological diversityof Magelang duck and genetic diversity using PCR-RFLP mtDNA D-loop region analysis of Magelangduck and four others native duck population (Tegal, Mojosari, Bali and Alabio duck) in Indonesia. Bloodsample was taken from 50 Magelang ducks and 20 of each native ducks. Morphological characteristicsof body measurement, production ability and egg quality of Magelang duck were analyzed usingCompletely Randomized Design with 11 plumage colors as treatments. PCR technique was administeredto amplify fragments in mtDNA D-loop region and PCR products were digested with endonucleaserestriction enzyme AluI and HaeIII. The result showed that morphology diversity of Magelang duck wasstatistically affected by different plumage colors. PCR-RFLP analysis using AluI and HaeIII restrictionenzyme resulted in six combinations of restriction fragment pattern shown in six haplotypes (A, B, C, D,E and F). Haplotype difference showed genetic diversity in the population of Magelang duck and theother native ducks. In conclusion, the different plumage colors affected morphology diversity ofMagelang duck. Genetic diversity of Indonesian native duck population could be identified by usingPCR-RFLP analysis on mtDNA D-loop region.
Sexual dimorphism and identification of single nucleotide polymorphism of growth hormone gene in muscovy duck Ismoyowati, I.; Tugiyanti, E.; Mufti, M.; Purwantini, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.167-174

Abstract

This research was aimed to investigate the different growth and to identify growth hormone gene polymorphism in Muscovy ducks. Two hundred Muscovy day-old ducks consisting of white-plumed male and female duck, black and white-plumed male and female ducks. Body weight was recorded weekly and the obtained data were subject to T test. Primer design used the Custal X Program based on a database from the GeneBank Cairina moschata GH gene, partial cds (AB158762). Primer base sequence of GH gene was forward/Sequence: 5’-CTGGGGTTGTTTAGCTTGGA-3’ and reverse/Sequence: 5’-TAAACCTTCCCTGGCACAAC-3’. The DNA sequences were aligned by using the BioEdit version 7.7 for identification of the single nucleotide polymorphism. The result showed that male Muscovy duck produced higher an average body weight gain and more relative growth than those of females. The highest body weight gain was at three weeks old, and then it started to decrease at four weeks old. The sequencing PCR product obtained nucleotide polymorphism. AA genotype was observed at 136 t of black female Muscovy duck, CC in black and white male Muscovy duck, and white female Muscovy duck. Conclusively, a body weight gain of 3-week-old male Muscovy ducks was higher than that of females and GH gene polymorphism was observed in Muscovy ducks.
Co-Authors E. Tugiyanti Henrik Henrik Ismoyowati Mufti, M. S. A. Santoso, S. A. T. Hartatik T. Yuwanta