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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Benny Rusli
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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POLA KETAHANAN (RESISTEN) DAN KEPEKAAN (SENSITIVITAS) KUMAN TERHADAP ANTIMIKROBA Y F Tallulembang; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.958

Abstract

In the developing countries including Indonesia, there still have problems with infectious diseases. Generally the hospitalized patientsin the Surgery section through have antimicrobial therapy. The treatment is intended to prevent nosocomial infection that usually foundin overnight treatment patients at the department of surgery. This study is carried out to know the pattern of micro-organism’s resistanceand sensitivity against various antimicrobial at department of surgery, Dr. Wahidin Sudirohusodo Hospital Makassar, January–June2008. A descriptive study of retrospectively collective data was carried out on 160 specimens by sensitivity test at the department ofsurgery, Dr. Wahidin Sudirohusodo Makassar Hospital, started from January up to June 2008. The five common bacterial found in thisstudy where Staphylococcus epidermidis, Klebsiella pneumonia, Enterobacter agglomerans, Staphylococcus saprophytic and Escherichiacoli. The sensitive antimicrobial drugs found where Vancomicin, Sulbactam/Cepoperazone, Cefepim, Gentamicin, Ceftazidim, Novobiocin,followed by the resistance antimicrobial were Methicillin, Tetracycline, Cefuroxim, Erythromycin, and Doxyciclin. The most bacterial typefound was Klebsiella pneumoniae, the most sensitive antimicrobial was Vancomicin and the most resistances were Methicillin.
RESISTENSI TERHADAP METHICILLIN (METHICILLIN RESISTANT) STAPHYLOCOCCUS AUREUS DI INSTALASI RAWAT INAP Wildana .; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1047

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. MRSA infection typically aggravatesthe patient condition. MRSA infection increases morbidity and mortality. The study was aimed to find out the MRSA occurrence inDr. Wahidin Sudirohusodo Hospital Makassar patients during July 2008–June 2009. A retrospective study was performed using datafrom the medical records including the results of culture and antimicrobial susceptibility test in Dr. Wahidin Sudirohusodo HospitalMakassar. Among 1082 results of the culture test, 5.2% were identified as Staphylococcus aureus, consist of 51.8% MSSA (MethicillinSensitive Staphylococcus aureus) and 48.2% MRSA. Most of the MRSA patients were treated in orthopaedic surgery (30%), internal(22%), and paediatric (19%) wards. Based on the clinical conditions, most of the patients were in post surgery care (44.4%), pneumonia(18.5%), and diabetic foot (7.5%). All of the MRSA isolates were multiresistant (resistant to three or more antimicrobials) but 96%remain sensitive to vancomycin. It was concluded that most of MRSA patients were staying in the orthopaedic surgery ward. Based onthis clinical condition, most of the patients were in the post surgery care. All of the MRSA isolates were multiresistant, but most of themremain sensitive to vancomycin.
BAKTERI AEROB DAN BAKTERI PENYEBAB PENYAKIT DI neonATAL InTenSIve CARe unIT Suriyanti Suriyanti; Irda Handayani; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.464

Abstract

Infectious diseases are still the main problem due to the potency of life threatening for neonates. Patients in the Neonatal Intensive Care Unit (NICU) are at risk for nosocomial infections resulting from the exposure to invasive procedures and medical equipments such as ventilator, catheter, Continuous Positive Airway Pressure (CPAP), incubator and antibiotics treatment. This study was aimed to know the related condition in NICU Dr. Wahidin Sudirohusodo Hospital Makassar by identification and determining the pathogenic bacteria colony counts of incubator and CPAP. The study design was a cross sectional method, by using incubator and CPAP as sample instruments, both equipments were sterilized at the NICU. The samples were collected and evaluated in July 2011. Samples were collected by usinga swab which was wiped to the instrument and put into Buffer Phosphate Saline (BPS); then diluted and put into Plate Count Agar (PCA) media for culture. The result of this study showed that there were no bacteria colony in the incubator, while in CPAP Acinetobacter calcoaceticus with bacteria colony count approximately 3.4×102 CFU/m3 were found. The researchers concluded that the incubator was sterile to bacteria, however in the CPAP bacteria from the strain of Acinetobacter calcoaceticus were found
KEPEKAAN ANTIMIKROBA KULTUR DARAH DI SEPSIS NEONATAL Tajuddin Noor; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.388

Abstract

Sepsis was one of the morbidity and mortality causes in neonatal. The diagnosis and treatment requires the bacterial identification and selection of sensitive antimicrobials. The aim of this study was to know the bacterial pattern and antimicrobial sensitivity of blood culture in the suspected neonatal sepsis patients who were treated at NICU in Dr.Wahidin Sudirohusodo Hospital Makassar. A retrospective study was conducted with secondary data from the culture and antimicrobial susceptibility test between the period of June 2010−July 2011. In this present study was found that from the total 91 blood culture isolates, bacteria Gram-negative group was 85.7% and Grampositive was 14.3% and the isolate encountered in order of frequency were Alkali genes faecalis 50.5%, Klebsiella pneumonia 25.3%, and Staphylococcus epidermidis 9.9%. In the Gram-negatives group, the isolate often encountered were Alkali genes faecalis 59.0%, Klebsiella pneumonia 29.4% and Enterobacter spp 6.4% while in the Gram-positive group were found Staphylococcus epidermidis 69.2% and Staphylococcus saprophytic 23.1%. The more sensitive antimicrobal that belong to Gram-negative group were Meropenem 94.4%, Levofloxacin 92.1%, and Ceftazidime 77.0% while the more resistant were Ampicillin 94.6%, Gentamycin 89.1% and Cefuroxime 82.7%. The more sensitive antimicrobal that belong to Gram-positive group were Vancomycin and Chloramphenicol 91.7% and Novobiosin 76.9% while the more resistant were Gentamycin and Ceftriaxone 100.0% and Amoxicillin 91.7%. Based on this study it can be concluded that Gram-negative aerobe bacteria was more common than the Gram-positive one. Meropenem, Levofloxacin and Ceftazidime antimicrobal were high sensitive to Gram-negative while Vancomycin, Chloramphenicol, and Novobiocin were high sensitive to Gram-positive. The resistance of Ampicillin and Gentamycin were found in both bacterially groups of sepsis suspected neonatal patients in NICU
EVALUASI AKTIVITAS TRANSAMINASE, DAN KADAR BILIRUBIN PADA PENDERITA VIRUS HEPATITIS B DAN C Yosepin .; Benny Rusli; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.996

Abstract

Hepatitis virus replicates only in hepatocyte. In hepatocyte injury, transaminase enzymes which normally intracellularly will transform to the blood circulation. And there are variation of increasing transaminase activities in HBV and HCV. To evaluatetransaminase activities, and bilirubin level in patients with HBV and HCV. Study design was cross sectional of 76 patients with HBVand HCV, data from medical record at Wahidin Sudirohusodo Hospital, Makassar, from January 2006 until July 2008 period. Datawas analyzed with Mann Whitney test using SPSS. windows versi on 12. of 76 patients with HBV and HCV were found 59 Men and 17women, age 15–72 years old. In there were no statistical significance between transaminase activites, bilirubin direct,indirect (p > 0.05),but there was significance difference of total bilirubin between HBV and HCV (p < 0.05). There were no statistical difference of AST,ALT, bilirubin direct and bilirubin indirect between HBV and HCV. Injury hepatocelluler because of HBV and HCV can not be identifiedjust by transaminase activities and bilirubin level, but there are other factor that influence.
ASPERGILLUS GLAUCUS GROUP DAN PENICILLIUM SP DI RUANG OPERASI BEDAH SARAF Nurul Hasanah; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1100

Abstract

Nosocomial infections occur widely in the world, most of them were in the poor and developing countries, because those infectiondiseases were still the mayor cause of high morbidity and mortality. All microorganisms including fungi may cause nosocomial infection.The fungal as opportunistic pathogens can threat immunocompromised patients such as neurosurgical patients and HIV/AIDS patients.The aim of this study was to identify the fungal species found in the neurosurgery and HIV/AIDS rooms at Dr. Wahidin SudirohusodoHospital Makassar. This study was a cross sectional study. The sample was the air in neurosurgery operating theater and HIV/AIDSward collected using Micro biology Air Sampler 100. The identification of fungal species using lacto phenol cotton blue stain were done inBalai Besar Laboratorium Kesehatan Makassar in the period of June up to July 2010. The amount of fungal colonies in the neurosurgeryroom was 36 CFU/m3 and the identified fungi were Aspergillus’s glaucus group and Penicillum sp. The amount range of fungal coloniesin HIV/AIDS ward were 102–158 CFU/m3 and the identified fungi were: Aspergillus’s Niger, Aspergillus’s glaucus group and Penicilliumsp. Based on this study it can be concluded that only Aspergillus’s glaucus and Penicillium sp were found in the neurosurgery operatingtheater and HIV/AIDS ward, while Aspergillus’s Niger was only found in the HIV/AIDS ward.
BAKTERI PATOGEN AEROB DAN UJI KEPEKAANNYA DI RUANGAN BEDAH PUSAT Agustini Agustini; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1073

Abstract

An operating room is potential in causing nosocomial infection in a hospital, especially due to surgical wound infections. Nosocomialinfection raises the length of day care and disease transmission risk. According to Permenkes No. 1204/Menkes/SK/X/2004, the operatingroom is a highly infection risk area. The aim of this study is to know the determination of the amount of bacterial colonies count, andthe sensitivity identification pattern of pathogenic bacteria in the central operating rooms at the Dr Wahidin Sudirohusodo Hospital.A cross sectional study was done by taking air samples using a Microbiology Air Sampler 100 in six central operating rooms in Dr.Wahidin Sudirohusodo Hospital. Counting bacterial colonies, identifying pathogenic bacteria and sensitivity test were done at the BalaiBesar Laboratorium Kesehatan Makassar (from May to June 2010). The result of the study shows that the amount of bacterial coloniesfound were approximately 148–440 CFU/m3 aerobic pathogenic bacteria consisting of Alcaligenes faecalis and Serratia liquefacienswhich were sensitive to sulbactam+ cefoperazone, gentamycin, levofloxacin but resistant to ampicillin, nitrofurantoin, clindamycin andmetronidazole. The researchers concluded that the amount of bacterial colonies found exceeded the Permenkes standard. The pathogenicbacteria were sensitive to sulbactam+ cefoperazone, gentamycin and levofloxacin however, resistant to ampicillin, nitrofurantoin,clindamycin, and metronidazole.
Co-Authors Agustini Agustini Hardjoeno . Irda Handayani Nurhayana Sennang Nurul Hasanah Suriyanti Suriyanti Tajuddin Noor Wildana . Y F Tallulembang Yosepin .