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PENGUMPULAN DAN BATAS PEMAKAIAN SAMPEL POPOK PADA PERBENIHAN URIN Rini Riyanti; Prihatini Prihatini; M.Y Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 2 (2005)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i2.847

Abstract

Urinary tract infection diagnosis is based on urine culture, taken from a midstream collection in the morning. Obtaining samplesin this manner is difficult in children less than 3 years. In children less than 3 years, urine is obtained by urine collectors. Using urinecollectors may cause discomfort, and the possibility that the urine collectors may not adhere resulting in contamination. (1) Developinga practical method for urine sample collection. (2) Comparing culture from diapers and urine collectors samples. (3) Knowing the limittime for using diapers acceptable for urine culture. Urine samples were obtained from 20 children less than 3 years, using urine collectorsand diapers used for 1 hour, 2 hours and 3 hours and then cultured. Majority of the urine culture from diapers used for 1 hour and 2hours showed the same result with the urine culture from urine collectors. Contamination was found in the urine culture result fromdiapers used for 3 hours. Urine samples from diapers used for 1 hour and 2 hours can be used as samples for urine culture. The techniqueis easy and can be done in children less than 3 years.
NILAI DIAGNOSTIK MALARIA ANTIGEN CASSETTE PENYAKIT MALARIA Binawati .; Prihatini .; M.Y Probohoesodo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.1031

Abstract

Malaria is an endemic disease in many countries. In 103 endemic countries with around 2.5 billion population, 1−3 million death cases were reported every year. Clinical criteria and blood smear established the diagnosis of malaria. ICT (imuno chromatographytest) is needed in peripheral areas where there are no experienced laboratory technicians. The procedure is simple, practical, easy, aswell as quicker than the conventional method, and no experienced technicians are needed this ICT advantages. The aim of this study isto know the diagnostic value of Malaria Antigen Cassette with microscopic examination as the gold standard examination of malaria.This research used observational cross-sectional method This study was done in Nusa Tenggara Barat during May−June 2008. Bloodsamples were taken by finger prick in patients with Malaria symptoms: fever, chill and sweating, followed by examining the blood smearby Malaria Antigen Cassette from Focus Diagnostic. The diagnostic value was then evaluated by calculating the sensitivity, specificity,positive predictive value as well as the negative predictive value. The diagnostic value of Malaria Antigen Cassette in patients withmalaria falciparum was found as follows: sensitivity 95.2%, specificity 100%, positive predictive value 100%, and negative predictivevalue 97.6%. The diagnostic value of Malaria Antigen Cassette in patients other types than malaria falciparum was as follows: sensitivity94.8%, specificity 100%, positive predictive value 100%, and negative predictive value 95.3%. The diagnostic value of Malaria AntigenCassette was very high in diagnosing malaria falciparum compared to other types than malaria falciparum.