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All Journal Journal of halal product and research (JHPR) Berkala Ilmiah Kimia Farmasi
Amirudin Prawita
Departemen Ilmu Kefarmasian, Fakultas Farmasi Universitas Airlangga, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Validation of UV Spectrophotometry Method for Determination of Lopinavir and Ritonavir Simultaneously Siti Umi Anisah; Asri Darmawati; Amirudin Prawita
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.225 KB) | DOI: 10.20473/bikfar.v8i2.31760

Abstract

Lopinavir and ritonavir are anti-viral compounds that have similar chemical structures and overlapping UV spectral profiles. The combination of these two compounds is being promoted as an anti-COVID19 drug. Determination of these two compounds simultaneously using UV spectrophotometry method requires special technique so that the result will be valid. The purpose of this study was to obtain a suitable analytical technique using UV spectrophotometry for the determination of lopinavir-ritonavir simultaneously that fulfill the method validation requirement. In this study, the simultaneous equation technique, absorptivity comparison factor, and first derivative technique were used to overcome the effect of lopinavir/ritonavir absorbance at selected wavelengths for determination of each compound simultaneously. The one-way ANOVA statistical test was used to compare the result of the three analytical techniques. The results showed that the three techniques fullfilled the AOAC requirements for selectivity and linearity. The accuracy and precision test result have not met the requirements of the AOAC method validation. Statistically. the one-way ANOVA analysis showed there was a significant difference between the mean recovery of lopinavir using the absorptivity factor and first derivative technique. Whereas, there was no significant differences among the mean of ritonavir recoveries that were determined using those three techniques. As conclusion, that the UV spectrophotometric method using the simultaneous equation technique, the absorptivity factor technique, and the derivative technique for assaying the lopinavir and ritonavir simultaneously met the requirements for selectivity and linearity parameters. However, the accuracy and precision have not met the requirements. The first derivative technique is suitable for further developed for ritonavir and lopinavir determination simultaneously
Validation gas chromatography-fid method for analysis of ethanol content in vinegar Indah Noviyanti Ruhmana Pulungan; Sugijanto Kartosentono; Amirudin Prawita
Journal of halal product and research (JPHR) Vol. 1 No. 2 (2018): Journal of Halal Product and Research (JHPR)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1097.727 KB) | DOI: 10.20473/jhpr.vol.1-issue.2.22-31

Abstract

Production process of vinegar could be synthesized by chemical or microbiological. In Islam, vinegar must not be consumed if contains ethanol ≥ 1%, because it is categorized into khamr. The maximum concentration of vinegar fermentation is 1,0 %. This research was to analyze the alcohol compund that consist in vinegar. The validation method that used in this study are: selectivity, linearity, range, precision, accuracy, LOD and LOQ. The analysis instrument was gas chromatography Agilent 6890 Series GC System, with a Flame Ionization Detector (FID) and a column HP-5 (5% Phenyl 95% Methyl Siloxane), and optimal conditions were obtained by using temperature of inlet and detector of 250°C whilst the temperature of oven programed initially at 40°C, hold for 5 minutes, and raised to 5°C/min to 200°C. were used in this study. For split ratio was achieved at 50:1 and the injection volume was 1.0µl. The methods were proven selective and demonstrated linearity, precision and accuracy with r = 0.9995; 5,63% and 101,25%, respectively. On the other hand, the methods were proven that the sample contains alcohol. Finally, the result of quantitative ethanol contents analysis in vinegar from Saudi Arabia is 2,28.10-2  % v/v; and vinegar brand “x” from market in Surabaya is 1,17.10-2 v/v.
Co-Authors Asri Darmawati Indah Noviyanti Ruhmana Pulungan Siti Umi Anisah Sugijanto Kartosentono