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Multiplex PCR for the detection of Salmonella spp. in Indonesian traditional shrimp paste (Terasi) Affan Gaffar; Yoga Dwi Jatmiko; Asep Awaludin Prihanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20227

Abstract

Terasi is a food condiment originating from Indonesia which is processed by fermenting shrimp, fish, or a mixture of both. The processing of terasi in Indonesia is still found traditionally so that it will affect the low quality of terasi which is characterized by being contaminated by pathogenic bacteria such as Salmonella. Salmonella is a bacterium that causes foodborne diseases. The purpose of this study was to evaluate the presence of Salmonella in shrimp paste using conventional method and then confirmed with molecular approach. This study used 10 samples consisting modern terasi and five traditional terasi. The isolation of Salmonella spp. carried out by using selective media, and then the isolates were characterized further using biochemical test (TSIA and LIA). The species identification of Salmonella spp. was carried out by multiplex PCR (mPCR). The isolates that were not detected by mPCR were further identified using 16s rDNA sequencing. The isolation results showed that Salmonella isolates were only detected in traditional terasi with a density range of 2.4 x 1052.9 x 108 CFU/g. A total of 20 isolates were characterized biochemically as Salmonella, and out of 17 isolates consistently identified as Salmonella enterica serovar Typhimurium by using mPCR. The rest three isolates were further identified as Citrobacter freundii based on 16s rDNA sequencing with similarity level of 99%. The presence of Salmonella in the shrimp paste indicates that the processing of traditional shrimp paste (terasi) should be evaluated in accordance with good manufacturing process.
IMPLEMENTASI PEMBELAJARAN MATEMATIKA REALISTIK UNTUK MENINGKATKAN KEMAMPUAN BERPIKIR KOMPUTASIONAL SISWA M. Gunawan Supiarmo; Nur Wiji Sholikin; Sri Harmonika; Affan Gaffar
Jurnal Numeracy Vol 9 No 1 (2022)
Publisher : Program Studi Pendidikan Matematika, STKIP Bina Bangsa Getsempena

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46244/numeracy.v9i1.1750

Abstract

Computational thinking is a type of problem-solving ability using logical thinking that students do with regular steps. This cognitive ability is one of the important skills in supporting students with mathematical concepts. However, the advantages of computational thinking do not seem to be paid much attention to by education, especially in Indonesia. This is because the learning approach does not emphasize the positive aspects that can improve students' computational thinking. As a result, the average computational thinking ability of students is low. This type of research uses an experimental method of pretest-posttest control group design. The population involved was class XII students at MA Daruttauhid Malang, which consisted of 22 students in the experimental class, and 24 students in the control class. The research data is in the form of pre-test scores before being given realistic mathematics learning treatment, and post-test score data. The results obtained showed that the computational thinking ability of students in the experimental class was higher than in the control class. To be clear, this fact is measured by calculating the N-Gain scores of students in the experimental class with a value of 0.7 (high category), and the N-Gain scores of control class students with a value of 0.5 (medium category). Abstrak Berpikir komputasional adalah jenis kemampuan pemecahan masalah menggunakan logika berpikir yang dilakukan siswa dengan langkah yang teratur. Kemampuan kognitif tersebut menjadi salah satu keterampilan penting dalam mendukung siswa terhadap konsep matematika. Namun keunggulan dari pemikiran komputasional, nampaknya tidak terlalu diperhatikan oleh Pendidikan, khususnya di Indonesia. Hal ini karena pendekatan pembelajaran kurang menekankan pada aspek positif yang dapat memberikan peningkatan siswa dalam berpikir secara komputasional. Akibatnya secara rata-rata kemampuan berpikir komputasional siswa menjadi rendah. Jenis penelitian ini menggunakan metode eksperimen jenis pretest-posttest control group design. Populasi yang terlibat ialah siswa kelas XII MA Daruttauhid Malang yang terdiri atas sebanyak 22 siswa pada kelas eksperimen, dan 24 siswa kelas kontrol. Data penelitian berupa skor pretest sebelum diberikan perlakuan pembelajaran matematika realistik, dan data skor posttest. Hasil penelitian yang diperoleh, menunjukkan bahwa kemampuan berpikir komputasional siswa pada kelas eksperimen lebih tinggi dibandingkan kelas kontrol. Untuk lebih jelasnya, fakta ini diukur dengan menghitung skor N-Gain siswa pada kelas eksperimen dengan nilai 0,7 (kategori tinggi), dan skor N-Gain siswa kelas kontrol bernilai 0,5 (kategori sedang).