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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) BioEksakta : Jurnal Ilmiah Biologi Unsoed
Dyah Fitri Kusharyati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Public Health Veterinary

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PEMERIKSAAN BAKTERI LEPTOSPIRA PADA SAMPEL DARAH MANUSIA SUSPECT LEPTOSPIROSIS MENGGUNAKAN METODE PCR (POLYMERASE CHAIN REACTION) Sefrita Tri Utami; Dyah Fitri Kusharyati; Hendro Pramono
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 9 Nomor 2 Desember 2013
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (797.897 KB) | DOI: 10.22435/blb.v9i2.819

Abstract

PEMERIKSAAN BAKTERI LEPTOSPIRA PADA SAMPEL DARAH MANUSIA SUSPECT LEPTOSPIROSIS MENGGUNAKAN METODE PCR (POLYMERASE CHAIN REACTION) Abstract ABSTRACTLeptospirosis is a zoonotic disease, which is caused by leptospira. Leptospirosis cases often show no specificclinical symptoms and is difficult to diagnose without testing samples in the laboratory. Testing using PCR(Polymerase Chain Reaction) is considered more accurate than the other methods. Components required in theexamination Leptospira bacteria in human blood samples using PCR method is DNA template, DNA polymeraseenzyme, forward primer (PU1 and SU1) and reverse primer (Lep R1), nuclease free water, Mg 2 +, and dNTPs.Examination of Leptospira bacteria in human blood samples include sampling, DNA isolation, examination byPCR, and electrophoresis running.Key words: leptospirosis, Leptospira, PCR methods ABSTRAKLeptospirosis adalah penyakit zoonosis yang disebabkan oleh bakteri Leptospira. Kasus leptospirosis seringtidak menunjukkan gejala klinis yang spesifik dan sulit didiagnosis tanpa pengujian sampel di laboratorium.Pengujian dengan menggunakan metode PCR (Polymerase Chain Reaction) dinilai lebih akurat dibandingkandengan metode yang lain. Komponen-komponen yang dibutuhkan dalam pemeriksaan bakteri Leptospira padasampel darah manusia menggunakan metode PCR adalah DNA template, enzim polymerase, Primer PU 1 danPrimer SU 1, Primer Lep R1, air, Mg2+ , dan dNTP. Pemeriksaan bakteri Leptospira pada sampel darah manusiameliputi pengambilan sampel, isolasi DNA, pemeriksaan dengan metode PCR, dan running elektroforesis.Kata kunci: leptospirosis, Leptospira, metode PCR
Penentuan Kualitas Air Waduk Cacaban, Tegal, Jawa Tengah Berdasarkan Parameter Mikrobiologi Fitri Amaliah; Diana Retna Utarini Suci Rahayu; Dyah Fitri Kusharyati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 4 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3496

Abstract

Waduk Cacaban terletak di Kecamatan Kedungbanteng Kabupaten Tegal, Jawa Tengah. Waduk Cacaban merupakan tempat yang banyak kegiatan manusia seperti kegiatan domestik, industri, dan kegiatan lainnya yang berdampak negatif terhadap sumberdaya air dan dapat menurunkan kualitas air. Salah satu cara untuk mengetahui kualitas perairan adalah berdasarkan analisis bakteri coliform. Tujuan dari penelitian ini adalah untuk menentukan kualitas air Waduk Cacaban berdasarkan parameter mikrobiologi, dan menentukan status perairan Waduk Cacaban berdasarkan Indeks Pencemaran. Metode yang digunakan pada penelitian ini adalah metode survei, sedangkan metode pengambilan sampel yaitu purposive sampling pada 3 stasiun (dermaga, inlet, dan keramba) dengan 2 kali pengambilan setiap stasiunnya. Parameter yang diamati yaitu bakteri coliform dan fecal coli dengan parameter pendukung yaitu pH, temperatur, dan Total Dissolved Solid (TDS). Analisis dilakukan berdasarkan parameter mikrobiologi sesuai standar dari SNI 06-4158-1996 dan status Waduk Cacaban berdasarkan Indeks Pencemaran sesuai KepMen LH Nomor 115 Tahun 2003 tentang Pedoman Penentuan Status Mutu Air. Perairan Waduk Cacaban memiliki temperatur berkisar antara 29,2 – 32,6˚C, TDS 137 - 156 mg/L. pH 6, total Coliform 565 – 2850 CFU/100 mL, dan fecal coli 0 – 425 CFU/100 mL. Status pencemaran pada dermaga Waduk Cacaban (inlet, dermaga, dan keramba) dalam kondisi baik dan memenuhi baku mutu.
Kualitas Yoghurt dengan Penambahan Bifidobacterium sp. Bb2E Yolandina Salsabila Putri; Dyah Fitri Kusharyati; Hendro Pramono
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.579 KB) | DOI: 10.20884/1.bioe.2020.2.1.1802

Abstract

Yoghurt merupakan produk pangan fungsional hasil fermentasi dari susu yang melibatkan peran bakteri asam laktat (BAL) Lactobacillus bulgaricus dan Streptococcus thermophillus. Menurut beberapa penelitian terdapat BAL yang memiliki ketahanan lebih baik dalam saluran pencernaan manusia, yaitu Bifidobacterium sp.. Selain itu, Bifidobacterium sp. memiliki manfaat diantaranya adalah meningkatkan fungsi pencernaan, menurunkan kolestrol, sebagai antioksidan dan anti inflamasi, mengurangi gejala alergi, serta dianggap sebagai salah satu bakteri probiotik yang penting dalam tubuh manusia. Tujuan dari penelitian adalah mengetahui pengaruh penambahan Bifidobacterium sp. Bb2E terhadap kualitas yoghurt dan mengetahui konsentrasi Bifidobacterium sp. Bb2E yang terbaik dari segi organoleptik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan, sehingga terdapat 12 unit percobaan. Perlakuan yang diterapkan adalah perbandingan konsentrasi starter yoghurt yang berbeda dari BAL L. bulgaricus, S. thermophillus dan substitusi Bifidobacterium sp. Bb2E dengan perbandingan 2:2:0 (Y0), 2:2:1 (Y1), 2:2:2 (Y2), dan 2:2:3 (Y3). Variabel dalam penelitian ini, variabel bebas, yaitu konsentrasi Bifidobacterium sp. Bb2E dan variabel terikat, yaitu kualitas yoghurt. Parameter utama adalah kadar asam laktat. Parameter pendukung, yaitu total BAL pada yoghurt, nilai pH, dan sifat organoleptik pada yoghurt. Data diolah dengan Analysis of Variance (ANOVA) dengan taraf kesalahan 5%. Penambahan Bifidobacterium sp. Bb2E tidak memberi pengaruh nyata terhadap kualitas yoghurt dari segi kadar asam laktat, pH, dan total BAL dan yoghurt dengan starter Lactobacillus bulgaricus : Streptococcus thermophillus : Bifidobacterium sp. Bb2E dengan perbandingan konsentrasi 2:2:3 merupakan yoghurt yang disukai konsumen dengan nilai rata-rata tertinggi pada uji organoleptik.
Pengaruh Penambahan Prebiotik Inulin dan Fruktooligosakarida (FOS) terhadap Pertumbuhan Probiotik Bifidobacterium sp. Bb2E Azma Nurizqi Isnasari; Dyah Fitri Kusharyati; Oedjijono Oedjijono
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3385

Abstract

Human health is very closely related to the condition of the human digestive tract since the beginning of life. The diversity of microorganisms found in the human digestive tract is very diverse, consisting of 300-500 different species of bacteria to increase the working power of nutrition. Prebiotics such as inulin and FOS and probiotics such as Bifidobacteria are aspects that can be added for the increase of nutrition. Optimal bacterial growth can be seen in the bacterial growth curve. The research problems were how the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, the amount of incubation time needed to support the growth of Bifidobacterium sp. Bb2E, and how the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. The purposes of this study were to study the effect of prebiotic inulin and FOS on the growth of Bifidobacterium sp. Bb2E, to know the optimal incubation time of Bifidobacterium sp. Bb2E, and to know the interaction between prebiotic types and incubation times on the growth of Bifidobacterium sp. BB2E. This research was conducted using a completely randomized design (CRD) with factorial patterns. The main parameter measured was the population of Bifidobacterium sp. Bb2E, and the additional parameters measured were the pH level and the value of lactic acid titrated. The independent variable discussed in this study is prebiotic estimation on the medium, while the dependent variable considered is the population of Bifidobacterium sp. BB2E. The results of this study showed that the addition of inulin and fructooligosaccharide prebiotic at different incubation times had a significant effect on the growth of Bifidobacterium sp. Bb2E. The best treatment was a combination of inulin + FOS at incubation time of 18 hours with an optical density value was 1,794 and a total population density was 2,44x1010 CFU/mL.
Metode Alternatif Penyimpanan Udang Segar dengan Nisin dari Lactococcus lactis sebagai Pengawet Alami Dyah Fitri Kusharyati; P Maria Hendrati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.284

Abstract

Nisin is a Lactococus lactis metabolite, one of natural preservatives against microbes. The antimicrobial inhibition is influenced by its concentration and contact time with the targeted microbes. The aim of the study was to know the concentration of L. lactis extract and optimal time of soaking the growing shrimp bacterial in refrigerator temperature for nine days. This study was conducted experimentally applying factorial design. The first factor was soaking duration, i.e. 0 minute (P0), 30 minutes (P1), 60 minutes (P2), and 90 minutes (P3), while the second factor was concentration levels of metabolite of L. lactis extract. The results showed that the metabolite has an inhibitory effect on shrimp bacterioal in dosed dependent way. The best performance of inhibition was detected at 60 minute-soaking duration.
Karakterisasi dan Optimasi Aktivitas Bakteriosin Isolat Bakteri Asam Laktat LG-90 Asal Sedimen Mangrove Pantai Logending Fiqita Mayliani; Dyah Fitri Kusharyati; Dini Ryandini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4575

Abstract

Lactic acid bacteria (LAB) are a group of Gram-positive bacteria that produce various active metabolites, including lactic acid, ethanol, hydroperoxides, and bacteriocins. Bacteriocin is a peptide that has a broad spectrum to inhibit the growth of pathogenic microbes. The ability of bacteriocins to inhibit microbial growth is influenced by various factors, including the concentration of antimicrobial substances, temperature, storage time, pH, and microbial properties. LG-90 isolated from mangrove sediments at Logending Beach located in Ayah Village, Ayah District, Kebumen Regency, is known to be capable of producing bacteriocins. This research aimed to determine the characteristics and identity of LG-90, the optimum time of bacteriocin production, and the optimum pH and temperature for bacteriocin activity of LG-90 as antimicrobial agents. This research used a survey method. The independent variable in this research was LAB LG-90 isolates and the dependent variable were the ability to produce bacteriocins and their antibacterial power. The main parameter observed was diameter of the inhibition zone and the supporting parameters were morphological, physiological, and biochemical properties of bacteria. Descriptive data analysis and characterization of bacterial isolates refers to Bergey’s Manual of Systematic Bacteriology. The results showed that LG-90 from the mangrove sediments of Logending Beach had the similar phenetic characters as the genus Lactobacillus. Optimum bacteriocin production of LG-90 at an incubation time of 16 hours. Optimum antimicrobial activity of LG-90 bacteriocin at pH 6 and heating temperature of 40oC.
Co-Authors Azma Nurizqi Isnasari Diana Retna Utarini Suci Rahayu, Diana Retna Utarini Suci Dini Ryandini Fiqita Mayliani Fitri Amaliah Hendro Pramono Oedjijono Oedjijono P Maria Hendrati Sefrita Tri Utami Yolandina Salsabila Putri