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Ogen Sea
Faculty of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Anthelmintic Activity of Basil Leaves (Ocimum sanctum Linn.) Infusion Against Ascaris suum In Vitro Ogen Sea; Mas'ud Hariadi; Setiawan Koesdarto; Kusnoto Kusnoto; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.215 KB) | DOI: 10.20473/jops.v1i2.16285

Abstract

Ascaris suum is a parasite nematode that causes infection in swines with high prevalence rates in host populations and usually associated with liver damages called “milk spots” caused by larvae migration, resulting in organ condemnation. Basil leaves (Ocimum sanctum) phytochemical constituents contains flavonoid, phenol and tannin. Tannins and phenolics are known to interfere with the energy generation in helminth parasites by uncoupling oxidative phosphorylation and also bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite and leading to death. This study was aimed to determine the activity of basil leaves (Ocimum sanctum) infusion in several concentrations against A. suum in vitro. This research used six treatments and four replications. This research used 10 A. suum in each treatment with four replication. The observations were done at 12, 18, 24, 30 and 36 hours in an incubator at 37oC. Based on the data analysis, basil leaves (Ocimum sanctum) infusion has anthelmintic activity against A. suum in vitro. The greater of the concentration and the longer of time of immersion, will make the death percentage of A. suum become higher. Concentration of basil leaves (Ocimum sanctum) infusion 15% is the effective concentration that can kill 100% of the A.suum during 36 hours of immersion.
Co-Authors Kusnoto Kusnoto Mas’ud Hariadi Ngakan Made Rai Widjaja Setiawan Koesdarto