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Latief Mooduto
Faculty of Dental Medicine Universitas Airlangga
Dentistry

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Effect of Epigallocatechin-3-Gallate (EGCG) on The Number of Lymphocyte Cells in Inflammation of Pulp with Mechanical Injury Kun Ismiyatin; Latief Mooduto; Putri Dea Amani Faadhilah
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (692.317 KB) | DOI: 10.20473/cdj.v10i1.2020.9-13

Abstract

Background: Pulpitis is an inflammatory pulp that can caused by pulp perforation by mechanical injury. Emergency treatment of pulpitis is using Eugenol. High concentrations of Eugenol have a cytotoxic effect that causes local necrosis and inhibits the healing process. Because of negative effects from eugenol, then it’s necessary to consider a new ingredient with minimal side effects, and it’s epigallocatechin-3-gallate (EGCG) in green tea. As a polyphenol, it has good antioxidant effect and plays a role in shortening the duration of inflammation by radical scavenging against Nitric Oxide so that NO levels rapidly decrease which causes migration of neutrophil cells to the area of injury will decrease and the inflammatory process faster so that the healing process become faster. Lymphocyte is plays a role in tissue repair. Purpose: to explain the effect of EGCG hydrogel on the number of lymphocyte cells in pulp inflammation with mechanical injury. Methods: The study used 24 Wistar rats divided into four groups, namely control (C), and 3 groups of treatment (T1, T2, T3). Each group consisted of 6 rats prepared, then EGCG hydrogel 60 ppm was named T1, EGCG hydrogel 90 ppm was named T2, EGCG hydrogel 120 ppm was named P3. On the 3rd day, Wistar rats were decapitated for HPA preparations. Results: This study showed a significant difference in each group (p < 0.05) using One-Way Anova analysis. Conclusion: EGCG hydrogel 90 ppm is effective in increasing the number of lymphocyte cells in inflammation of the pulp with mechanical injury. 
Hemisection of a severely decayed mandibular molar: Case report Cendranata Wibawa Ongkowijoyo; Latief Mooduto; Deavita Dinari; Riski Setyo Avianti
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2064.912 KB) | DOI: 10.20473/cdj.v10i1.2020.23-26

Abstract

Introduction: Dental implants gained popularity as the treatment to replace grossly decayed teeth. However, with the increasing evidence and the difficulty to manage complications associated with dental implants, clinicians are pushed to opt for a more conservative approach. Case report: A male patient with a chief complaint of frequent food impaction in a severely decayed mandibular molar wished to retain his tooth. Upon thorough examination, the carious lesion extended to furcal area that rendered the distal root unsalvageable. However the mesial root can be retained; thus, hemisection was proposed. Management: Root canal treatment was carried out in the mesial canals. Then, the tooth was split mesio-distally.The distal root was extracted, and the mesial root was retained. Subsequently, the tooth was restored with PFM crown.Conclusion: Hemisection with subsequent prosthetic rehabilitation can be a viable alternative to retain severely decayed mandibular molar.
Antibacterial potency of mangosteen pericarp extracts (Garcinia mangostana L.) against Fusobacterium nucleatum Nia Pramais Octaviani; Latief Mooduto; Achmad Sudirman
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.44-47

Abstract

Background: Fusobacterium nucleatum is a common bacterial in root canal with pulp necrosis and periradicular lesion. A way to eliminate these bacteria from root canal is by root canal irrigation. Root canal irrigation materials that are widely used nowadays has many shortcomings. The pericarp of mangosteen (Garcinia mangostana L) has antibacterial potency. Therefore mangosteen pericarp can be an alternative material which could inhibit and bactericidal function to Fusobacterium nucleatum. Purpose: The aim of this study was to determine the antibacterial potency of mangosteen pericarp extract (Garcinia Mangostana L.) against Fusobacterium nucleatum. Methods: This study was laboratory experimental with pos-test only control group design. A microdilution method was used to determine minimum inhibitory concentration and minimum bactericidal concentration by colony counting bacteriae in Tryptone Soya Agar (TSA) media with drop plate technique. Growth of bacterial colonies in TSA is calculated manually in colony forming unit (CFU/ml). Results: Bacterial colonies growth at concentration 0.78% was 90% less than positive control group and there were no bacterial colonies growth at concentration 0.975%. Conclusion: The Minimum Inhibitory Concentration (MIC) of mangosteen pericarp against Fusobacterium nucleatum was at 0,78% concentration and the Minimum Bactericidal Concentration (MBC) was at 0.975% concentration.
Co-Authors Achmad Sudirman Cendranata Wibawa Ongkowijoyo Deavita Dinari Kun Ismiyatin Nia Pramais Octaviani Putri Dea Amani Faadhilah Riski Setyo Avianti