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Tiara Meilena
Faculty of Dental Medicine Universitas Airlangga
Dentistry

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Toxicity Test on Taro Leaf Extract (Colocasia Esculenta L. Schoot) as Mouthwash to BHK-21 Fibroblast Cell Culture in Denture Users Tiara Meilena; Januar Cidie Fabiansyah; Eha Djulaeha; Hanoem Eka Hidayati
Indonesian Journal of Dental Medicine Vol. 1 No. 1 (2018): January - June
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.923 KB) | DOI: 10.20473/ijdm.v1i1.2018.35-39

Abstract

Background: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that must be conducted is in vitro tests, one of which is MTT Assay. Samples used in this research were fibroblast cells (cultured BHK-21 cells) since fibroblasts are the most important and largest components in pulp, periodontal ligament, and gingiva. Purpose: This research aimed to determine toxicity effect of taro leaf extracts (Colocasia esculenta L. Schoot) on BHK-21 fibroblast cells. Methods: This research is an experimental laboratory research using Post Test Only Control Group design.  The research treatment are the administration of Taro leaf extract (Colocasia esculenta L. Schoot)  with 100%, 80%, 60%, and 40% concentration on BHK-21 fibroblast cell. Results: The fibroblast cell life percentage in 100%, 80%, 60%, and 40% concentration respectively are 106,3%, 50,9%, 36,2%, and 30,1%. The toxicity results are obtained using MTT assay technique after 24 hours. The optical density absorbency values are read by ELISA reader and represent life cell viability. Conclusion: Taro leaf extract (Colocasia esculenta L. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cells
Co-Authors Eha Djulaeha Hanoem Eka Hidayati Januar Cidie Fabiansyah