Ning Rintiswati
Department of Microbiology, Faculty of Medicine, Universitas Gajdah Mada, Yogyakarta, Indonesia

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Effect of Oxidative Stress on AhpC Activity and Virulence in katG Ser315 Thr Mycobacterium tuberculosis Mutant Rintiswati, Ning; Wibawa, Tri; Asmara, Widya; Soebono, Hardyanto
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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AbstractMycobacterium tuberculosis strains resistance to INH is mainly caused by the alteration in several genesencoding the molecular targets. Mutation of katG at codon 315 especially Ser315Thr are responsible forINH resistance in a large proportion of TB cases. The aim of this study is to evaluate the influence of stressoxidative on AhpC activity of katG Ser315Thr of M.tuberculosis, and to find out the relation of AhpC and thevirulence of this mutant. The study design was laboratoric experimental, subjects of study were M.tuberculosisINH resistance strains, and the treatment were serial dose of H2O2. Eighty five M.tuberculosis INH resistantclinical strain were screened for mutation of katGSer315Thr by PCR/RFLP and characterized on the basis ofphenotypic properties (catalase activity and AhpC activity). AhpC activity of katG Ser315Thr M.tuberculosisstrains in response to oxidative stress condition was evaluated by culturing the strains on liquid culturemedium containing 1mM H2O2. To ascertain role of AhpC in the virulence of katGSer315Thr mutant strains, themutants were infected into human macrophages culture, and several indicator of virulence were observed (i.e:replication competence, and apoptosis induction on human macrophages). The results showed that katG Ser315Thr were identified in 23 (27,05%) of 85 INH resistance strains, all mutant strains had decrease of catalaseactivity. AhpC activity of katG Ser315Thr of M.tuberculosis increased significantly with increase of hydrogenperoxide dose. In addition , it has been shown that increased AhpC activity related to replication ability ofmutant, and reduction of apoptosis macrophages induction significantly. We conclude that the productionof AhpC of katG Ser315Thr M.tuberculosis induced by oxidative stress. There was a role of AhpC in virulenceof the M.tuberculosis katG Ser315Thr strains by replication capability and macrophages apoptosis.Keywords : katG Ser315Thr Mycobacterium tuberculosis- oxidative stress - AhpC - virulence
Faktor-Faktor Yang Berpengaruh Terhadap Kejadian Tuberkulosis Pada Wanita di Kabupaten Cilacap Provinsi Jawa Tengah Sumarna, Nana; Rintiswati, Ning; Lazuardi, Lutfan; Lazuardi, Lutfan
Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Vol 4 No 2 (2015): Jurnal Ilmu Kesehatan Bhakti Husada
Publisher : Sekolah Tinggi Ilmu Kesehatan Kuningan

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Background : Tuberculosis (TB) is disease a second major cause of deaths worldwide among infectious diseases, killing nearly 2 million people each year. The variety of problems will arise if the woman was suffering TB especially who are married, pregnant, and have children. Her role as a housewife who had to carry out physical or mental care of children while taking care of her husband will be disturbed. The prevalence of smear positive pulmonary Tuberculosis (TB) in 6 region health center in Cilacap District at 2012, women higher than men.Objective : To know probability of pregnancy, marital status, parity, physical activity, level of education, level of knowledge, the kitchen smoke pollution, history contact with TB patient, residential density, ventilation against TB incidence in women.Method : The type of research was observational analytical case-control design. Sample is 102 people consisting of as many as 51 cases and 51 control. Sampling is done with proportional random sampling techniques. Data were analyzed with the univariabel, bivariabel analysis, and multivariable. Results : Pregnancy (OR 1.2 95% CI 2.4-23.7 p0.04), parity (OR 3.5, 95% CI 1.3-9.7 p 0.01), history of contacts (OR 3.8 95% CI 1.4-10.4 p 0.01) ventilation (OR 2.4 95% CI 7,5-23.7 p 0.00) as a risk factor for the occurrence of TB in women. Conclusion : The incidence of TB in women in Cilacap District a more probable or risk greater in women who were pregnant, high parity, had a history of contacts, and stay at home with the bad ventilation.
Pengaruh Pelatihan Mikroskopis Tuberkulosis Terhadap Kualitas Sediaan dan Slide Positivity Rate di Wilayah Kerja Dinas Kesehatan Kabupaten Purbalingga Kurniawan, Dede; Rintiswati, Ning; Pramono, Dibyo
Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Vol 4 No 2 (2015): Jurnal Ilmu Kesehatan Bhakti Husada
Publisher : Lembaga Penelitian Sekolah Tinggi Ilmu Kesehatan Kuningan

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Introduction: Microscopic examination of sputum is a key component of the diagnosis of tuberculosis. In Purbalingga district, was conducted in 24 peripheral laboratories. Five technicians were trained in 2011-2012 and 12 technicians in 2003-2009 by National Tuberculosis Programs. Seven technicians have not trained. In 2011 slide positivity rate an average of 9.1%, 73.4% poor smear quality and 11.5% poor staining, 1.4% error rate at 34.7% laboratoriy. In 2012 slide positivity rate an average of 8.9, evenness was the highest of poor smear quality (77.7%), and 4.6% error rate at 66,7% laboratory. Therefore, need refresher training and initial training. Objective: To determine impact of training to smear quality and slide positivity rate. Methods: The research design was analytic quasi experimental with 5 days microscopic training. The research subject were 18 technician microscopic. They were divided into experimental and control group each 9 technicians with purposive sampling. The differences of smear quality and slide positivity rate were analyzed by independent t-test and Mann-Whitney test with 95% CI. Results: Training has increased the average of knowledge test score 40.7 points and average of test score the good smear quality : specimen quality increased 90 points, staining 84.4, cleanness 85.6, thickness 91.1, smear size 88.9, evenness 87.8 and increase the average of smear readings score test 22 points. The mean of smear quality and slide positivity rate of the experimental group were higher than control group. Statistical test of smear quality between eksperimental group with contol group were: specimen quality p=0.03, staining quality p=0.03, cleanness p=0.02, thickness p<0.001, size p<0.001, good evenness p<0.001, and slide positivity rate p=0.02 Conclusion: The mean of smear quality and slide positivity rate of the experimental group were higher than control group, there were significant differences of smear quality and slide positivity rate between experimental group with control group at 3 months after training.