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All Journal Jurnal Surya Masyarakat Jurnal Entomologi Indonesia
Ign. Joko Suyono
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Cenderawasih
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Other

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The effect of DMSO on ITS2 amplification in the molecular identification of Anopheles farauti Laveran (Diptera: Culicidae), from a colony established in the laboratory Ign. Joko Suyono; Jesmandt Situmorang; R.C. Hidayat Soesilohadi; Rarastoeti Pratiwi
Jurnal Entomologi Indonesia Vol 10 No 2 (2013): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.779 KB) | DOI: 10.5994/jei.10.2.93

Abstract

Sibling species identification is very important in the understanding of malaria epidemiology. Morphological criteria are usually used in the identification of anopheline species, but this fails when sibling or cryptic species occur. Analysis by PCR-RFLP of rDNA ITS2 is currently the most reliable and sensitive method for distinguishing between members of the Anopheles punctulatus group. The objective of this study was to investigate the effect of DMSO concentration on ITS2 amplification of An. farauti from the colony maintained at BATAN Jakarta using PCR-RFLP based on the rDNA ITS2. The results showed that the addition of 6 % and 7 % DMSO produced ITS2 amplification products in the size 750 bp. DMSO could be used in PCR to relieve secondary structures when amplifying high GC templates. Molecular identification of An. farauti is found to be Anopheles farauti sensu stricto.
E-Commerce Papua Tani sebagai Marketplace Hasil Pertanian Distrik Skanto Kabupaten Keerom Papua Mingsep Rante Sampebua; Ign Joko Suyono
Jurnal Surya Masyarakat Vol 4, No 2 (2022): Mei 2022
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jsm.4.2.2022.169-174

Abstract

The purpose of the service activity is to implement an online business model, namely e-commerce Papua tani as a marketplace or agricultural products in Keerom Regency. E-commerce Papua tani is a sustainable business model because e-commerce Papua tani can be developed for various business services which include online marketing services, agricultural fertilizer and pharmaceutical services, online marketing agents, business capital for farmers through cooperatives, and extension services, agriculture in partnership with the Keerom Regency Government. The implementation of community service activities is using the lecture method, discussion, question and answer, demonstration, and practice of using the e-commerce Papua tani application. The result of the service activity is the web-based application as a marketplace or agricultural products in the Skanto Disacetrict, Keerom Regency. The e-commerce Papua tani application is used as a medium for the promotion and marketing of farmers' products in the Skanto District which can be marketed online directly to consumers at the right price without going through middlemen. This can increase the income and welfare of farmers in the Skanto District, Keerom District. The implementation of the Papua Tani e-commerce application using tokopedia.com business platform makes it easy for people to sell agricultural products in the Skanto district, Keerom Regency.
Co-Authors Aditya K. Karim Jesmandt Situmorang Mingsep Rante Sampebua R.C. Hidayat Soesilohadi Rarastoeti Pratiwi