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Yora FARAMITHA
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Agriculture, Biological Sciences & Forestry

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Molecular identification and phylogenetic analysis of Chlorella isolates from Indonesia using rbcL gene Fauziatul FITRIYAH; Yora FARAMITHA; Dini Astika SARI; Irma KRESNAWATY; Tri PANJI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 89, No 1 (2021): April, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.408

Abstract

Identifying the newly isolated species is crucial to establishing a reliable algal database with successful commercial applications for different biotechnological applications. Morphological identification does not give sufficient description, especially for tiny unicellular microalgae. The rbcL gene encodes the large unit of ribulose-1, 5-bisphosphate carboxylase /oxygenase (Rubisco) has been widely known for barcoding in plants and developed for microalgae molecular identification. In this study, we examined the local strains of green microalgae from Indonesia using the rbcL partial gene sequence to identify the strains. Green microalgae isolates originated from Yogyakarta, Serayu, Gondol, Ancol, Cilegon, and Teluk Jakarta were cultured in f/2 media and harvested for DNA extraction. The DNA extracted was proceeded to PCR using 1AB_rbcL primer pair to amplify the sequences of rbcL gene with target band located at 582 bp, followed by the sequencing of the PCR product was conducted. Molecular identification of local green microalgae isolates was successfully carried out using primers 1AB_rbcL with a genetic similarity of 99% toward identified species in the NCBI database. Among six isolates, TJ, G, S, C, and A isolates were identified as C. pyrenoidosa. Only CP isolate from Yogyakarta identified as C. sorokiniana. Nannochloropsis gaditana rbcL sequence was selected as an outgroup. The phylogenetic analysis indicated that the five isolates of Chlorella belong to one clade and clearly distinguished from C. sorokiniana isolate from Yogyakarta.
Sintesis gliserol ester berbasis asam oleat sawit dan karakteristik sifat fisika kimia Firda DIMAWARNITA; Erliza HAMBALI; Tri PANJI; . MUSLICH; Yora FARAMITHA
E-Journal Menara Perkebunan Vol 89, No 2 (2021): Oktober, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i2.464

Abstract

Peningkatan kapasitas produksi biodiesel menyebabkan meningkatnya produksi gliserol. Salah satu cara alternatif untuk meningkatkan nilai ekonomi gliserol adalah melalui esterifikasi gliserol menjadi surfaktan, yaitu gliserol ester (GE). Penelitian ini bertujuan melakukan sintesis GE berbasis asam oleat sawit dengan waktu reaksi 180 menit menggunakan katalis p-toluenesulfonic acid (PTSA). Suhu sintesis yang digunakan, yaitu:140, 160, 180, dan 240°C. Hasil uji ANOVA dan Duncan (α = 0,05) menunjukkan bahwa suhu sintesis berpengaruh nyata pada rendemen, angka asam, dan viskositas. Proses sintesis GE optimum diperoleh pada suhu sintesis 160°C dengan nilai rendemen GE sebesar 95,66%, pH 7, nilai angka asam 11,28 mg KOH/g sampel, viskositas kinematis 75,76 cst, dan densitas 0,944 g cm-3. Suhu sintesis 160°C, 180°C, dan 240°C menghasilkan rendemen yang tinggi, berkisar 95,66-97,07% dan tidak berbeda nyata antara ketiganya. Analisis menggunakan metode Analytical Hierarchy Process(AHP) juga menghasilkan suhu terbaik untuk sintesis GE adalah 160°C. Produk GE yang terbentuk mengandung gugus ester, asam karboksilat, dan alkohol yang muncul pada 1240,41-1739,62 cm-1.[Kata kunci: biodiesel, esterifikasi, katalis PTSA, surfaktan]  
Co-Authors . MUSLICH Dini Astika SARI Djoko Santoso Erliza Hambali Fauziatul FITRIYAH Firda DIMAWARNITA Irma KRESNAWATY Tri Panji