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All Journal JPTK: Jurnal Pendidikan Teknologi dan Kejuruan Neo-Bis Jurnal Dinamika Sosial Budaya Jurnal Riset Ekonomi dan Bisnis Menara Perkebunan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) SOLUSI Jurnal Tanah dan Iklim JIKAP (Jurnal Informasi Dan Komunikasi Administrasi Perkantoran) Unisia Sustainable Business Journal

Djoko Santoso

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Author-ID : 255068

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Medicine & Pharmacology Neuroscience Social Sciences Other

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PENGARUH PERSON-ORGANIZATION FIT(P-O FIT) TERHADAP ORGANIZATION CITIZENSHIP BEHAVIOUR (OCB) DENGAN KEPUASAN KERJA DAN KOMITMEN ORGANISASI SEBAGAI VARIABEL INTERVENING (Studi pada KPPBC TMP Tanjung Emas Semarang) Djoko Santoso; Isnu Irwantoro
Neo-Bis Vol 8, No 1 (2014): JUNI
Publisher : Trunojoyo University of Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/nbs.v8i1.476

Penelitian ini bertujuan untuk menganalisis Organization Citizenship Behaviour ( OCB ) dari petugas KPPBC TMP di Tanjung Emas . Penelitian ini menggunakan variabel Person - Organization Fit ( P-O Fit ) untuk menganalisis pengaruh Organization Citizenship Behaviour ( OCB ) dengan menggunakan kepuasan kerja dan komitmen organisasi sebagai variabel intervening . Populasi dalam penelitian ini adalah seluruh petugas KPPBC TMP di Tanjung Emas Semarang sebanyak 246 petugas dengan tingkat pendidikan mereka di sekolah tinggi setidaknya antara usia 20 sampai 55 tahun dan memiliki kemampuan di bidang masing-masing pekerjaan dan dalam pandangan peneliti memiliki jenjang karir yang baik. Berdasarkan populasi sebanyak 246 petugas ditentukan sampel sebanyak 170 petugas dengan menggunakan metode pengambilan sampel secara stratified random sampling, yang tersebar di seluruh unit kerja di KPPBC TMP Tanjung Emas Semarang . Data penelitian diperoleh dengan menggunakan teknik wawancara dan kuesioner sensus , dengan memberikan kuesioner dalam pertanyaan terbuka dan wawancara dengan responden secara langsung . Teknik analisis dalam penelitian ini adalah analisis SEM ( Structure Equation Modelling ) yang dioperasikan dengan menggunakan AMOS 21 . Hasil penelitian ini menunjukkan bahwa variabel Person - Organisasi Fit ( P-O Fit ) memiliki dampak yang signifikan dan positif terhadap kepuasan kerja dan variabel komitmen organisasi tetapi dampaknya tidak signifikan dan negatif terhadap variabel Organization Citizienship Behavior ( OCB ) . Variabel kepuasan kerja berpengaruh signifikan dan positif terhadap variabel komitmen organisasi . Selanjutnya , kepuasan kerja dan variabel komitmen organisasi memiliki dampak yang signifikan dan positif terhadap variabel Organization Citizienship Behavior ( OCB ) .

KAJIAN POLA PEMBIAYAAN USAHA PADA UMKM EKONOMI KREATIF KOTA SEMARANG Indarto Indarto; Djoko Santoso; Aprih Santoso
Jurnal Dinamika Sosial Budaya Vol 19, No 2 (2017): Desember
Publisher : Universitas Semarang

UMKM adalah pelaku penting dalam industri ekonomi kreatif di Indonesia. Potensi UMKM dalam ekonomi kreatif di Indonesia sangat besar mengingat Indonesia sangat kaya dengan keragaman kesenian dan kebudayaan.Demikian juga dengan potensi UMKM ekonomi kreatif di Kota Semarang. Harus diakui bahwa perkembangan UMKM ekonomi kreatif masih relatif tertinggal dari dibandingkan dengan perkembangan UMKM ekonomi kreatif di kota-kota seperti; Bandung, Yogyakarta malang dan lain-lain. Penelitian ini bermaksud mengkaji apakah kinerja usaha UMKM ekonomi kreatif di Kota Semarang yang masih tertinggal dibanding kota-kota lain berkaitan dengan pola pembiayaan UMKM itu sendiri. Penelitian ini menggunakan metode kualitatif dengan pendekatan fenomenologis. Informan dalam penelitian adalah 12 pelaku UMKM Ekonomi Kreatif di Kota Semarang. Pengumpulan data dengan metode wawancara, observasi dan dokumentasi. Hasil penelitian menunjukkan bahwa dengan pendekatan fenomenologis ditemukan 2 pola pembiayaan usaha yang diterapkan oleh pelaku UMKM Ekonomi Kreatif yaitu pola pembiayaan usaha yang cenderung menghindari risiko dan pola pembiayaan pembiayaan yang berani mengambil risiko.Pola pembiayaan usaha yang berani mengambil risiko menunjukkan perkembangan usaha yang lebih pesat daripada pola pembiayaan usaha yang menghindari risiko.

PERAN TEAMWORK DAN TIME PRESSURE TERHADAP KINERJA DENGAN AFFECTIVE COMMITMENT SEBAGAI VARIABEL INTERVENING Candra Auromiqo; Indarto Indarto; Djoko Santoso
Jurnal Riset Ekonomi dan Bisnis Vol 12, No 3 (2019): DESEMBER
Publisher : Universitas Semarang

Tujuan penelitian untuk mengetahui pengaruh teamwork dan time pressure terhadap affective commitment yang berdampak pada kinerja auditor BPK Perwakilan Provinsi Jawa Tengah. Variabel yang digunakan adalah teamwork dan time pressure sebagai variabel independen, kinerja sebagai variabel dependen serta affective commitment sebagai variabel intervening. Sampel yang digunakan adalah auditor BPK Perwakilan Provinsi Jawa Tengah yang berjumlah 100 responden, pengambilan sampel menggunakan purposive sampling. Metode pengumpulan data yang digunakan adalah kuesioner (angket). Pengolahan data menggunakan PLS. Analisis yang digunakan meliputi outer model, uji validitas, uji reliabilitas, inner model, dan uji hipotesis. Berdasarkan hasil analisis dapat diketahui bahwa teamwork dan time pressure berpengaruh terhadap affective commitment, time pressure berpengaruh terhadap kinerja tetapi teamwork tidak berpengaruh terhadap kinerja, dan affective commitment bukan sebagai variabel intervening hubungan antara variabel teamwork dan time pressure terhadap kinerja auditor BPK Perwakilan Provinsi Jawa Tengah.The aim of the study was to determine the effect of teamwork and time pressure on affective commitment which had an impact on the performance of the auditors in BPK Representative Office of Central Java. Variables used are teamwork and time pressure as independent variables, performance as dependent variable and affective commitment as intervening variables. The sample used was the auditor in BPK Representative Office of Central Java, amounting to 100 respondents, sampling using purposive sampling. Data collection method used is questionnaire. The analysis used includes outer model, validity test, reliability test, inner model, and hypothesis test. Based on the results of the analysis it can be seen that teamwork and time pressure have an influence on affective commitment, time pressure have an influence on performance but teamwork has no effect on their performance, and affective commitment is not an intervening variable of the relationship between teamwork and time pressure variable on the performance of the BPK Representative Office of Central Java Auditors.

APOPTOSIS INDEX BETWEEN FEMALES AND MALES IN REGULAR HEMODIALYSIS Djoko Santoso
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.410

Many reports have documented apoptosis index in hemodialysis patients, but to date, no single study has directly compared the apoptosis index of males to females. Data on mortality rate among hemodialytic patients in the hemodialysis center at the Department of Internal Medicine Dr Soetomo General Hospital, Surabaya, Indonesia show a high number predominated by female patients. Therefore, to answer the question of whether there is a gender difference in apoptosis index, the researcher studied leukocyte responses in male and female hemodialysis patients. The apoptosis index of the sample was measured by indirect immunoassay method. Cell lyses, followed by immunochemical determination of histone-complexed DNA fragments in a microtiter plate wells. The apoptosis quantization was obtained by determining the amount of colored product spectrophotometrically. One hundred and four non-diabetic subjects who received hemodialysis (HD), and 24 normal controls (NC), were evaluated. The apoptosis index in ESRD patients group and control group showed no significant difference (0.6172 vs 0.4008, p=0.114), neither did it vary in both sexes and age groups. When the sex factor was analyzed (after exclusion from the diabetic ESRD patients), females apoptosis index was significantly higher than that of the males (0.7325 vs 0.55175, p<0.05). In conclusion, apoptosis index in females among non-diabetic patients undergoing hemodialysis is higher than that occur in males and controls.

Nutrient Balance and Economic Analyses of P Fertilization on an Acid Upland Soil in Sumatra DJOKO SANTOSO; I GEDE PUTU WIGENA; R.D.B LEFROY
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) No 19 (2001): Desember 2001
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v0n19.2001.%p

Soil and crop responses to P fertilization were assessed for seven seasons, from rainy season 1997/1998 until rainy season 2000/2001, in a field experiment at Pauh Menang village of Jambi province, Indonesia. An incomplete factorial combination of six levels of inorganic P (0, 19, 38, 57, 76 and 95 kg P ha-1 as SP-36), two sources organic matters (FYM and stylo), and lime, was laid out in a randomized complete block design with four replicates. The soil in this site was very P deficient and application of 38 kg P ha-1 increased corn yields significantly from less than 1.0 to about 3.5 t ha-1 for the first four crops. The higher rates of applied P didn’t increase further corn yields, indicating that external P requirement was fulfilled. A build-up and maintenance rate of banded application of SP-36,that required in this soil was 38 and 19 kg P ha-1, respectively. Broadcast application for the entire topsoil layer in a plot or farmland would require considerably larger amount of P fertilizer for building up of P level than the banded application. The balances of P treatments were all positive and substantiated by the increasing Colwell P content of the soil with increasing rate of applied P. Although no apparent symptom of Zn deficiency was observed, the Zn content in plant leaf decreased with increasing rate of applied P. By assuming all labour costs as cash costs in conducting economic analyses to calculate the B/C ratios, in general only the SP-36 treatments gave beneficial returns. Although many of the B/C ratios were less than 1.0, the amount of total benefits that could be earned by farmers were considerably high because the total production costs included all labour costs.

Penggunaan biostimulan Orgamin untuk efisiensi pemupukan dan peningkatan produktivitas kelapa sawit di dataran tinggi Application of Orgamin biostimulan to enhance fertilizer efficiency and productivity of oil palm grown in highland Happy WIDIASTUTI; Djoko SANTOSO; Soekarno Mismana PUTRA; Memed WIRAMIHARDJA; Aida FARIDA; B. MARAHIMIN MARAHIMIN; K. PANJAITAN PANJAITAN; Jisman SINAGA
E-Journal Menara Perkebunan Vol 81, No 2: Desember 2013
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractThe extension of oil palm area has been expanded tomarginal land such as the highland regions. However, theproductivity of the oil palm became the main demand for theplanters. Increasing of oil palm productivity can be done byapplication of growth regulators. Growth regulators aresmall molecules in a relatively very small amount that affectthe growth and development of plant. This study wasconducted to asses the efectiveness of plant growth regu-lators (Orgamin and Orgamin plus) in improving fertilizerefficiency and productivity of mature oil palm (TM 7). Theexperiments were conducted at Marjandi oil palm plantationat an altitude of 700 m above sea level in a total area of 16 ha. Six treatments tested were 1). 100% inorganicfertilizer (control), 2). 50% inorganic fertilizer + Orgamin(50K+O), 3). 75% inorganic fertilizer + Orgamin (75K+O),4). 50% inorganic fertilizer + Orgamin plus (50K+OP), 5).75% inorganic fertilizer + Orgamin plus (75K+OP), and 6).100% inorganic fertilizer + Orgamin plus (100K+OP)arranged in a randomized block design (RBD) with threereplications. Orgamin (O) and Orgamin plus (OP) wereapplied in the hole around the oil palm along with inorganicfertilizers. The results showed that application of O and OPimproved the efficiency of inorganic fertilizers by 50% basedon vegetative variables and increased the concentration ofN, P, and K of leaf and soil compared to those of 100%inorganic fertilizer. In addition to the height and leaf numberof plant parameters, the leaf of the plant treated with O andOP showed more greenish compared to those of control.There is an indication that the O application increased thepercentage of female flowers. In addition the application ofOrgamin also produced the highest oil content in oil palmfruit particularly in the treatment of 75% of inorganicfertilizer + orgamin harvested in October compared to thosein March. Moreover, application of OP increased both thetotal weight and weight per bunch of FFB.AbstrakPengembangan kelapa sawit mengharuskan pengguna-an lahan suboptimal seperti daerah dataran tinggi. Produk-tivitas kelapa sawit menjadi tuntutan utama bagi pekebun.Peningkatan produktivitas kelapa sawit di dataran tinggididuga dapat dilakukan dengan aplikasi zat pengatur tumbuh.Zat pengatur tumbuh merupakan molekul “kecil” (small molecules) yang dalam jumlah relatif sangat sedikit mem-pengaruhi pertumbuhan/perkembangan tanaman. Penelitiandilakukan untuk menguji formula zat pengatur tumbuh(Orgamin dan Orgamin plus) dalam meningkatkan efisiensipemupukan dan produktivitas kelapa sawit TM 7. Percobaandilakukan di kebun Marjandi dengan ketinggian 700 dpl padaareal seluas 16 ha. Enam perlakuan yang diuji adalah 1).pupuk anorganik 100% (100K), 2). pupuk anorganik 50% +Orgamin (50K+O), 3). pupuk anorganik 75% + Orgamin(75K+O), 4). pupuk anorganik 50% + Orgamin plus (50K+OP), 5). pupuk anorganik 75% + Orgamin plus (75K+OP),dan 6). pupuk anorganik 100% + Orgamin plus (100K+OP)yang disusun dalam rancangan acak kelompok (RAK)dengan tiga ulangan. Orgamin (O) dan Orgamin plus (OP)diberikan dalam lubang di piringan pokok bersamaan denganpupuk anorganik. Hasil pengamatan menunjukkan bahwapemberian O dan OP dapat meningkatkan efisiensi pemupuk-an anorganik hingga 50% dilihat dari beberapa peubahvegetatif dan menghasilkan kadar N, P, dan K daun dantanah lebih tinggi dibandingkan dengan pemberian pupukanorganik 100%. Selain pada parameter tinggi tanaman danjumlah daun, peningkatan juga terlihat pada tingkatkehijauan daun. Terdapat indikasi bahwa pemberian Orgaminmeningkatkan persentase jumlah bunga betina. PemberianOrgamin juga menghasilkan kadar minyak tertinggi khusus-nya pada pemberian Orgamin + pupuk anorganik 75% padabuah yang dipanen bulan Oktober dibandingkan dengan buahyang dipanen bulan Maret. Baik data bobot per tandanmaupun bobot TBS menunjukkan bahwa pemberian OPdapat meningkatkan kedua peubah tersebut.

Aktivitas ACCase mesokarp kelapa sawit dan kloning fragmen gen penyandi ACCase subunit biotin karboksilase ACCase activity of oil palm mesocarp and cloning of gene fragment encoding biotin carboxylase subunit of ACCase Asmini BUDIANI; Djoko SANTOSO; Hajrial ASWIDINNOOR; Antonius SUWANTO
E-Journal Menara Perkebunan Vol 74, No 1: Juni 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Genetic engineering to produce high yielding oil palm might be done by over expressing gene encoding key enzyme for oil biosynthesis in the oil palm mesocarp, one of which is ACCase. The objective of this research was to analyze ACCase activity of mesocarp from several developmental stages of fruit and to clone conserved region cDNA of gene encoding biotin carboxylase subunit of ACCase (BC-htACCase) from oil palm mesocarp. Activity of ACCase was analyzed by HPLC. Amplification of cDNA was done by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate heterologous primer on several annealing temperature and MgCl2 concentration. The cDNA fragment of RT-PCR product was cloned, sequenced and analyzed to confirm that the cloned cDNA was conserved region of BC-htACCase. The result showed that ACCase activity increased from the 14 week to the 20 week-old fruit, and then decreased. Using heterologous degenerate primers, cDNA fragments of BC-htACCase conserved region (469 bp) can be specifically amplified at 60 oC annealing temperature with 2 mM MgCl2 concentration.The result of BlastX analysis showed that the sequence of cloned cDNA fragment was highly homologous with the conserved region of BC-htACCase from Glycine max, Arabidopsis thaliana, Nicotiana tabacum, and Brassica napus with 243, 237, 236, 231 bit score, and E. value 2e-63, 1e-61, 2e-61 and 5e-60, respectively. Ringkasan Rekayasa genetika untuk menghasilkan bibit kelapa sawit berdaya hasil tinggi dapat ditempuh dengan meningkatkan ekspresi gen penyandi enzim kunci biosintesis minyak pada kelapa sawit, salah satunya adalah ACCase. Tujuan penelitian ini adalah menguji aktivitas ACCase mesokarp beberapa tahap perkem-bangan buah sawit dan mengklon fragmen cDNA daerah konservatif gen penyandi ACCase heteromerik subunit biotin karbok-silase (BC-htACCase) dari mesokarp buah sawit. Aktivitas ACCase dianalisis dengan HPLC. Amplifikasi cDNA dilakukan dengan teknik RT-PCR menggunakan primer degene-rate heterologus pada berbagai suhu penempelan dan konsentrasi MgCl2. Fragmen cDNA hasil RT-PCR diklon, disekuen dan dianalisis untuk mengkonfirmasi bahwa cDNA terklon adalah daerah konservatif BC-htACCase. Hasil penelitian menunjukkan bahwa aktivitas ACCase meningkat dari buah berumur 14 minggu hingga buah berumur 20 minggu, kemudian menurun kembali Dengan primer degenerate heterologus, fragmen cDNA daerah konservatif BC-htACCase (469 pb) dapat diamplifikasi secara spesifik pada suhu penempelan 60 oC dan konsentrasi MgCl2 2 mM. Hasil analisis BlastX dari sekuen DNA fragmen terklon menunjuk-kan bahwa sekuen tersebut mempunyai homologi tinggi antara lain dengan gen penyandi BC-htACCase dari Glycine max, Arabidopsis thaliana, Nicotiana tabacum dan Brassica napus, masing-masing dengan skor 243, 237, 236, 231 bit, dan E. value 2e-63, 1e-61, 2e-61 dan 5e-60.

Molecular identification and phylogenetic analysis of Chlorella isolates from Indonesia using rbcL gene Fauziatul FITRIYAH; Yora FARAMITHA; Dini Astika SARI; Irma KRESNAWATY; Tri PANJI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 89, No 1 (2021): April, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.408

Identifying the newly isolated species is crucial to establishing a reliable algal database with successful commercial applications for different biotechnological applications. Morphological identification does not give sufficient description, especially for tiny unicellular microalgae. The rbcL gene encodes the large unit of ribulose-1, 5-bisphosphate carboxylase /oxygenase (Rubisco) has been widely known for barcoding in plants and developed for microalgae molecular identification. In this study, we examined the local strains of green microalgae from Indonesia using the rbcL partial gene sequence to identify the strains. Green microalgae isolates originated from Yogyakarta, Serayu, Gondol, Ancol, Cilegon, and Teluk Jakarta were cultured in f/2 media and harvested for DNA extraction. The DNA extracted was proceeded to PCR using 1AB_rbcL primer pair to amplify the sequences of rbcL gene with target band located at 582 bp, followed by the sequencing of the PCR product was conducted. Molecular identification of local green microalgae isolates was successfully carried out using primers 1AB_rbcL with a genetic similarity of 99% toward identified species in the NCBI database. Among six isolates, TJ, G, S, C, and A isolates were identified as C. pyrenoidosa. Only CP isolate from Yogyakarta identified as C. sorokiniana. Nannochloropsis gaditana rbcL sequence was selected as an outgroup. The phylogenetic analysis indicated that the five isolates of Chlorella belong to one clade and clearly distinguished from C. sorokiniana isolate from Yogyakarta.

Kloning cDNA lengkap penyandi ACCase subunit biotin carboxylase dari mesokarp kelapa sawit (Elaeis guineensis Jacq.) Cloning of full length cDNA encoding ACCase subunit biotin carboxylase from mesocarp of oil palm (Elaeis guineensis Jacq.) Asmini BUDIANI; Antonius SUWANTO; Hajrial ASWIDINNOO; Djoko SANTOSO; Basil J NIKOLAU
E-Journal Menara Perkebunan Vol 81, No 2: Desember 2013
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractAcetyl-CoA Carboxylase (ACCase) is considered to beone of the key enzymes in palm oil biosynthesis. Availabilityof genes encoding this enzyme would give some advantagesin the molecular breeding of oil palm. Over expression ofthe genes in the oil palm mesocarp might increase the oilproduction in this tissue. On the other hand, downregulating of ACCase could divert the central metaboliteAcetyl-CoA to other product such as PHB (Polyhydroxy-butyrate), one of the known biodegradable plastic. Thispaper reported the work of cloning of the full length codingsequence of biotin carboxylase (BC), one subunit of theACCase. Based on the DNA sequence of the BC conservedregion that had cloned previously, primers pairs weredesigned to amplify 5’- and 3’- cDNA ends of BC usingRACE-PCR. The RACE products of 5’- and 3’- cDNA endsof BC were cloned into E.coli, and the DNAs weresequenced and analysed. The full cDNA of BC was obtainedby reisolation of the cloned 5’- and 3’- cDNA ends followedby digestion using KpnI, ligation into pGEM-T vector andcloning into E.coli. Colony PCR was carried out to confirmthat the target gene has been cloned. The recombinantplasmid containing full cDNA of BC was then isolated forDNA sequencing. The results showed that the 5’-BC (1367bp), 3’- BC (1032 bp), and the full length cDNA encodingBC (2182 bp) had been successfully cloned, and the DNAsequence had been confirmed as gene encoding ACCasesubunit biotin carboxylase.AbstrakAcetyl-CoA Carboxylase (ACCase) merupakan salahsatu enzim kunci dalam biosintesis minyak sawit. Keter-sediaan gen penyandi enzim ini sangat berguna dalampemuliaan kelapa sawit secara molekuler. Over-ekspresi genpenyandi ACCase pada mesokarp dapat meningkatkan pro-duksi minyak pada jaringan tersebut. Sebaliknya ekspresiACCase dapat ditekan melalui mekanisme down regulation sehingga metabolit central Acetyl-CoA dapat diarahkanuntuk menghasilkan produk lain seperti PHB (polyhydro-xybutyrate), salah satu jenis biodegradable plastik yangtelah banyak dikenal. Penelitian ini bertujuan untukmengklon cDNA lengkap penyandi ACCase subunit biotincarboxylase (BC) dari mesokarp kelapa sawit. Berdasarkansekuen DNA daerah konservatif BC yang telah diklon darimesokarp kelapa sawit pada penelitian sebelumnya, duapasang primer dirancang untuk mengamplifikasi daerahujung 5’- dan 3’- cDNA BC dengan RACE-PCR. Produk5’-RACE dan 3’-RACE diklon dan disekuen. cDNAlengkap penyandi BC diperoleh dengan jalan mengisolasikembali fragmen 5’- dan 3’- cDNA terklon, dilanjutkandengan digesti menggunakan enzim restriksi KpnI, ligasikedua fragmen ke vektor kloning pGEM-T, dan introduksike dalam E. coli. Setelah dilakukan PCR koloni untukmenguji keberhasilan kloning, plasmid rekombinan yangmengandung cDNA lengkap dari BC diisolasi untuk analisissekuen DNA. Dari penelitian ini fragmen cDNA 5’-BC(1367 pb) dan 3’- BC (1032 pb), serta cDNA lengkappenyandi BC berukuran 2182 pb telah diperoleh dan diklondalam E. coli. Analisis sekuen DNA mengkonfirmasi bahwacDNA terklon adalah benar gen penyandi ACCase subunitbiotin carboxylase.

Deteksi dan analisis sekuen gen inhibitor proteinase pada beberapa klon kakao harapan tahan penggerek buah kakao dari Sulawesi Selatan Detection and sequence analysis of proteinase inhibitor gene in cacao clones putatively cacao pod borer-tolerant from South Sulawesi Abdul Mollah S. JAYA; Hajrial ASWIDINNOOR; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 72, No 1: Juni 2004
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Cacao is socially and economically an important commodity for Indonesia, in which the cacao plantations have been challenged with a threatening pest, cacao pod borer (CPB). This research aimed to identify and clone PIN (proteinase inhibitor), a gene carrying resistance of plant to some chewing pests like CPB. The methodology included several experiments. Detection of PIN in cacao was done by PCR using PIN-specific heterologous primers and cacao genomic DNA as templates. Cloning vector pGEM-T was utilized to clone the PCR products. Sequence analysis was conducted with BlastX and Blast Special programs from NCBI. Alignment analysis to determine genetic similarity was performed with ClustalW from EBI. Thirteen of the 18 clones tested were detected to have PIN homologs. Two DNA fragments from cacao clones putatively tolerant to CPB, MJ-1 and LW-1, were sequenced. One of them, MJ-1 was cloned. Sequence analyses of the fragments of both cacao clones, indicated that they have PIN homologs and a very closed genetic relation with 96% level of similarity. Ringkasan Kakao adalah komoditas yang secara sosial maupun ekonomi penting bagi Indonesia, dimana perkebunan kakao menghadapi masalah serius hamapenggerek buah kakao (PBK). Penelitian ini bertujuan mengidentifikasi dan mengklon PIN (inhibitor proteinase), gen yang membawa sifat ketahanan tanaman terhadap hama ulat seperti PBK. Metodologinya terdiri dari beberapa percobaan. Deteksi PIN di dalam kakao dikerjakan dengan PCR menggunakan primer heterologous yang spesifik terhadap PIN dan DNA genomik kakao sebagai templetnya. Vektor kloning pGEM-T digunakan untuk mengklon produk PCR. Analisis sekuen dilakukan dengan program BlastX dan Blast spesial dari NCBI. Analisis penjajaran (alignment) untuk menentukan kemiripan genetik menggunakan program ClustalW dari EBI. Tiga belas dari 18 klon kakao yang diuji menunjukkan adanya homolog PIN. Dua DNA fragmen dari klon harapan tahan, MJ-1 dan LW-1, telah ditentukan sekuen nukleotidanya. Satu diantara-nya, MJ-1 berhasil diklon. Analisis sekuen kedua klon tersebut menunjukkan identitas sebagai homolog PIN dan keduanya memiliki kemiripan genetik yang tinggi.

Co-Authors . Mansyurdin . SAMANHUDI . SYAFARUDDIN A. H. SARAGIH SARAGIH A.R. PURBA PURBA Abdul Mollah S. JAYA Agustina A. HANDAYAN Aida Farida Ajeng Nuzulia H Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Anwar, Aswaldi Aprih Santoso Asmini Budiani Auzar SYARIEF B. MARAHIMIN MARAHIMIN Bambang S PURWOKO Basil J NIKOLAU Candra Auromiqo Christantius Dwiatmadja CITRA ANDRIANI KUSUMAWAI Deden Dewantara ERIS DEDI SOLEH EFFENDI Dini Astika SARI Efendi, Darda F NURILMALA Fauziatul FITRIYAH GALUH WENING PERMATASARI Hajrial ASWIDINNOO HAJRIAL ASWIDINNOOR Hanny Hafiar Happy Widiastuti HAPPY WIDIASTUTI Hayati MINARSIH Hayati Minarsih I GEDE PUTU WIGENA Imron Riyadi Indarto Indarto Indarto SE Irma KRESNAWATY Isnu Irwantoro Jisman SINAGA K. PANJAITAN PANJAITAN M. A. GHONI GHONI Mayta Novaliza Isda Memed WIRAMIHARDJA Muhammad Jusuf Muhammad Munir Musliar Kasim Niyyah FITRANTI Niyyah FITRANTY Oktaviany Ferry TRIASTANTO Patni Ninghardjanti R.D.B LEFROY Rita Hayati Riza A PUTRANTO Riza A. PUTRANTO ROEDHY POERWANTO SATRIYAS ILYAS Soekarno Mismana Putra Sukarti Moeljopawiro Tetty CHAIDAMSARI Tri Panji Umi Rochayati VS Ariyanto Yora FARAMITHA Yuniarti Anis Pramukawati

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