Claim Missing Document
Check
Articles

Found 28 Documents
Search

Application of organic fungicide in controlling basal stem rot disease for mature oil palm Happy WIDIASTUTI; Hayati MINARSIH; Djoko SANTOSO; Deden Dewantara ERIS; Galuh Wening PERMATASARI
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.537 KB) | DOI: 10.22302/iribb.jur.mp.v88i1.368

Abstract

Ganoderma is a major pathogen in oil palm crops. Some efforts related to control the growth of Ganoderma have been conducted but still have not found an effective method. This study aims to develop an organic fungicide that has been tested in vitro, which effective in controlling the growth of Ganoderma. The optimization carried out includes the determination of the dose and time interval for application in 13-year-old mature oil palm. This organic fungicide application was the continuation of application during the previous year especially for the two best treatment which is application organic fungicide every week (1w) and every two weeks (2w). In this study, the treatments tested were three levels dose of organic fungicide (0, 1x and 2x) and two types of frequency application, i.e. every week (1w) and every other week (2w). The results showed that the best application of organic fungicides was every week application with twice doses (1w.2x), based on the parameters of the inhibition of Ganoderma’s fruiting body formation, primary and secondary root formation, the opening of spear leaves, and harvesting parameters. The application of organic fungicide able to recover the oil palm infected Ganoderma sp., with increasing the fresh fruit bunch and its weight around 70% and 78%, respectively.
PENGARUH PERSON-ORGANIZATION FIT(P-O FIT) TERHADAP ORGANIZATION CITIZENSHIP BEHAVIOUR (OCB) DENGAN KEPUASAN KERJA DAN KOMITMEN ORGANISASI SEBAGAI VARIABEL INTERVENING (Studi pada KPPBC TMP Tanjung Emas Semarang) Djoko Santoso; Isnu Irwantoro
Neo-Bis Vol 8, No 1 (2014): JUNI
Publisher : Trunojoyo University of Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/nbs.v8i1.476

Abstract

Penelitian ini bertujuan untuk menganalisis Organization Citizenship Behaviour ( OCB ) dari petugas KPPBC TMP di Tanjung Emas . Penelitian ini menggunakan variabel Person - Organization Fit ( P-O Fit ) untuk menganalisis pengaruh Organization Citizenship Behaviour ( OCB ) dengan menggunakan kepuasan kerja dan komitmen organisasi sebagai variabel intervening . Populasi dalam penelitian ini adalah seluruh petugas KPPBC TMP di Tanjung Emas Semarang sebanyak 246 petugas dengan tingkat pendidikan mereka di sekolah tinggi setidaknya antara usia 20 sampai 55 tahun dan memiliki kemampuan di bidang masing-masing pekerjaan dan dalam pandangan peneliti memiliki jenjang karir yang baik. Berdasarkan populasi sebanyak 246 petugas ditentukan sampel sebanyak 170 petugas dengan menggunakan metode pengambilan sampel secara stratified random sampling,  yang tersebar di seluruh unit kerja di KPPBC TMP Tanjung Emas Semarang . Data penelitian diperoleh dengan menggunakan teknik wawancara dan kuesioner sensus , dengan memberikan kuesioner dalam pertanyaan terbuka dan wawancara dengan responden secara langsung . Teknik analisis dalam penelitian ini adalah analisis SEM ( Structure Equation Modelling ) yang dioperasikan dengan menggunakan AMOS 21 . Hasil penelitian ini menunjukkan bahwa variabel Person - Organisasi Fit ( P-O Fit ) memiliki dampak yang signifikan dan positif terhadap kepuasan kerja dan variabel komitmen organisasi  tetapi dampaknya tidak signifikan dan negatif terhadap variabel Organization Citizienship Behavior ( OCB ) . Variabel kepuasan kerja berpengaruh signifikan dan positif terhadap variabel komitmen organisasi . Selanjutnya , kepuasan kerja dan variabel komitmen organisasi memiliki dampak yang signifikan dan positif terhadap variabel Organization Citizienship Behavior ( OCB ) .
Kloning cDNA lengkap penyandi ACCase subunit biotin carboxylase dari mesokarp kelapa sawit (Elaeis guineensis Jacq.) Cloning of full length cDNA encoding ACCase subunit biotin carboxylase from mesocarp of oil palm (Elaeis guineensis Jacq.) Asmini BUDIANI; Antonius SUWANTO; Hajrial ASWIDINNOO; Djoko SANTOSO; Basil J NIKOLAU
E-Journal Menara Perkebunan Vol 81, No 2: Desember 2013
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.363 KB) | DOI: 10.22302/iribb.jur.mp.v81i2.43

Abstract

AbstractAcetyl-CoA Carboxylase (ACCase) is considered to beone of the key enzymes in palm oil biosynthesis. Availabilityof genes encoding this enzyme would give some advantagesin the molecular breeding of oil palm. Over expression ofthe genes in the oil palm mesocarp might increase the oilproduction in this tissue. On the other hand, downregulating of ACCase could divert the central metaboliteAcetyl-CoA to other product such as PHB (Polyhydroxy-butyrate), one of the known biodegradable plastic. Thispaper reported the work of cloning of the full length codingsequence of biotin carboxylase (BC), one subunit of theACCase. Based on the DNA sequence of the BC conservedregion that had cloned previously, primers pairs weredesigned to amplify 5’- and 3’- cDNA ends of BC usingRACE-PCR. The RACE products of 5’- and 3’- cDNA endsof BC were cloned into E.coli, and the DNAs weresequenced and analysed. The full cDNA of BC was obtainedby reisolation of the cloned 5’- and 3’- cDNA ends followedby digestion using KpnI, ligation into pGEM-T vector andcloning into E.coli. Colony PCR was carried out to confirmthat the target gene has been cloned. The recombinantplasmid containing full cDNA of BC was then isolated forDNA sequencing. The results showed that the 5’-BC (1367bp), 3’- BC (1032 bp), and the full length cDNA encodingBC (2182 bp) had been successfully cloned, and the DNAsequence had been confirmed as gene encoding ACCasesubunit biotin carboxylase.AbstrakAcetyl-CoA Carboxylase (ACCase) merupakan salahsatu enzim kunci dalam biosintesis minyak sawit. Keter-sediaan gen penyandi enzim ini sangat berguna dalampemuliaan kelapa sawit secara molekuler. Over-ekspresi genpenyandi ACCase pada mesokarp dapat meningkatkan pro-duksi minyak pada jaringan tersebut. Sebaliknya ekspresiACCase dapat ditekan melalui mekanisme down regulation sehingga metabolit central Acetyl-CoA dapat diarahkanuntuk menghasilkan produk lain seperti PHB (polyhydro-xybutyrate), salah satu jenis biodegradable plastik yangtelah banyak dikenal. Penelitian ini bertujuan untukmengklon cDNA lengkap penyandi ACCase subunit biotincarboxylase (BC) dari mesokarp kelapa sawit. Berdasarkansekuen DNA daerah konservatif BC yang telah diklon darimesokarp kelapa sawit pada penelitian sebelumnya, duapasang primer dirancang untuk mengamplifikasi daerahujung 5’- dan 3’- cDNA BC dengan RACE-PCR. Produk5’-RACE dan 3’-RACE diklon dan disekuen. cDNAlengkap penyandi BC diperoleh dengan jalan mengisolasikembali fragmen 5’- dan 3’- cDNA terklon, dilanjutkandengan digesti menggunakan enzim restriksi KpnI, ligasikedua fragmen ke vektor kloning pGEM-T, dan introduksike dalam E. coli. Setelah dilakukan PCR koloni untukmenguji keberhasilan kloning, plasmid rekombinan yangmengandung cDNA lengkap dari BC diisolasi untuk analisissekuen DNA. Dari penelitian ini fragmen cDNA 5’-BC(1367 pb) dan 3’- BC (1032 pb), serta cDNA lengkappenyandi BC berukuran 2182 pb telah diperoleh dan diklondalam E. coli. Analisis sekuen DNA mengkonfirmasi bahwacDNA terklon adalah benar gen penyandi ACCase subunitbiotin carboxylase.
Respons awal pemberian biostimulan Orgamin pada kelapa sawit (Elaeis guineensis Jacq.) di Kebun Marjandi PTPN IV Early response of Orgamin biostimulan application in oil palm (Elaeis guineensis Jacq.) at PTPN IV Marjandi plantation Soekarno Mismana PUTRA; Djoko SANTOSO; Happy WIDIASTUTI; A. H. SARAGIH SARAGIH; M. A. GHONI GHONI; B. MARAHIMIN MARAHIMIN; K. PANJAITAN PANJAITAN
E-Journal Menara Perkebunan Vol 81, No 1: Juni 2013
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.51 KB) | DOI: 10.22302/iribb.jur.mp.v81i1.51

Abstract

AbstractEffort to increase the production of oil palm can beconducted through application of plant growth regulator(PGR). Orgamin biostimulan is a natural PGR formulathat has been tested to improve the vegetative growths ofcorn and oil palm in the glass house. Assessment ofOrgamin and Orgamin plus (Orgamin + micro nutrient)applications at commercial scale was carried out inMarjandi oil palm plantation of PTPN IV usingrandomized block design with three treatments, i.e. K =100% recommended dose of inorganic fertilizer(control), O= Orgamin (1.5 kg/tree) + 50% dose ofinorganic fertilizer, OP = Orgamin plus (1.5 kg/tree)without inorganic fertilizer. The parameters ofobservation at 2.5 months after the treatments were soiland leaf nutrient contents (N, P, K, Mg), percentage offemale flower, mesocarp oil content, and harvested freshfruit bunches (FFB). The observation showed that therewas an increased in oil yield, weight of FFB and leafnutrient content, while the percentage of female flowerand nutrient content of soil were not significantlydifferent compared to the control.AbstrakUpaya untuk meningkatkan produksi kelapa sawitdapat dilakukan antara lain melalui pemberian zatpengatur tumbuh (ZPT). Biostimulan Orgamin merupa-kan formula ZPT alami yang telah diuji di rumah kacapada tanaman jagung dan bibit kelapa sawit. Uji cobaaplikasi Orgamin dan Orgamin plus (Orgamin yangdiperkaya hara mikro) pada skala lapang dilakukan dikebun kelapa sawit Marjandi PTPN IV denganmenggunakan Rancangan Acak Kelompok (RAK) untukmenguji tiga perlakuan, yaitu 1) K (kontrol) = 100%dosis anjuran pupuk kimia (APK = kontrol), 2) O = 50%dosis APK + Orgamin (1,5 kg/pohon), 3) OP = Orgaminplus (1,5 kg/pohon) tanpa pupuk kimia. Peubah yangdiamati pada 2,5 bulan setelah perlakuan adalah kan-dungan hara tanah dan daun (N, P, K, Mg), persentasebunga betina, rendemen minyak mesokarp, dan produksitandan buah segar (TBS). Hasil yang diperoleh menunjukkan terdapat peningkatan rendemen minyak, bobotTBS dan kandungan hara daun, sedangkan persentasebunga betina dan kandungan hara tanah tidak menunjuk-kan perbedaan yang nyata antara perlakuan dan kontrol.
Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods) Imron Riyadi; Darda EFENDI; Bambang S PURWOKO; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.376 KB) | DOI: 10.22302/iribb.jur.mp.v1i1.258

Abstract

AbstractA right combination of cytokinin is able to support the process of callus differentiation to somatic embryo formation in plant somatic embryogenesis. Liquid culture application could increase the efficiency of in vitro culture process on plants. This research aimed to determine the best concentration of TDZ combined with kinetin for callus differentiation to  somatic embryo of sago palm on three culture methods. Plant material used was embryogenic callus derived from tips meristem culture from sucker of Alitir sago palm. Callus was cultured on modified MS media added with: 0.0, 0.1, 0.5 and 1.0 mg/L TDZ combined with 0.5 mg/L kinetin for 12 weeks with subcultures every 6 weeks. Three culture methods used were suspension, temporary immersion system (TIS), and solid media. There were 12 treatments with 4 replicates. The results showed that the highest number of somatic embryos was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in 6 weeks (167.3 embryos/flask) and 12 weeks (389.2 embryos/flask) with its fresh weight of 18.4 g and 29.1 g, respectively. The highset survival rate in final culture (12 weeks) was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin (100%). The shortest time for somatic embryos expression was achieved on TIS culture with 1.0 mg/L TDZ and 0.5 mg/L kinetin in two weeks after culture. Histological analysis of early-stage somatic embryos showed the presence of dense and compact cellular arrangements which formed growth spot axis for shoot or SAM (shoot apical meristem) and root or RAM (root apical meristem) that connected each other. [Key words: culture method, embryogenic callus, Metroxylon sagu Rottb., kinetin, sago palm, TDZ]   AbstrakAplikasi kombinasi sitokinin yang tepat dapat mendorong proses diferensiasi kalus membentuk embrio somatik pada proses embriogenesis somatik tanaman. Penggunaan metode kultur cair dapat meningkatkan efisiensi proses kultur in vitro tanaman. Penelitian ini bertujuan untuk menentukan konsentrasi TDZ terbaik dikombinasikan dengan kinetin dalam proses diferensiasi kalus membentuk embrio somatik tanaman sagu pada tiga metode kultur. Bahan tanam penelitian  berupa kalus embriogenik tanaman sagu asal kultur meristem pucuk dari anakan sagu jenis Alitir. Kalus dikulturkan pada media modifikasi dengan penambahan  TDZ dengan konsentrasi 0,1; 0,5; dan 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L selama 12 minggu yang disubkultur pada umur 6 minggu. Metode kultur yang digunakan terdiri atas tiga macam yaitu: kultur suspensi, sistem perendaman sesaat (SPS) dan media padat. Perlakuan terdiri atas 12 kombinasi perlakuan dengan empat ulangan. Hasil penelitian menunjukkan bahwa rerata jumlah embrio somatik tertinggi dicapai pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L baik pada umur kultur 6 minggu (167,3 buah) maupun umur 12 minggu (389,2 buah). Rerata bobot segar tertinggi juga diperoleh pada perlakuan metode kultur SPS dengan TDZ 1,0 mg/L  pada umur kultur 6 minggu (18,4 g) dan  12 minggu (29,1 g). Rerata daya hidup kultur akhir (12 minggu) tertinggi  sebesar 100% diperoleh pada perlakuan SPS. Induksi embrio somatik  tercepat yakni setelah  dua minggu diperoleh pada  metode kultur SPS dengan TDZ 1,0 mg/L dikombinasikan dengan kinetin 0,5 mg/L. Analisis histologi embrio somatik stadium awal  menunjukkan adanya susunan sel yang rapat dan kompak yang menyusun semacam poros atau berkas titik tumbuh tunas atau SAM (shoot apical meristem) maupun akar atau RAM (root apical mersitem) yang saling terhubung.[Kata kunci: kalus embriogenik, metode kultur, kinetin, TDZ, sagu, Metroxylon sagu]
Keragaman sekuen DNA fragmen gen penyandi ACCase subunit BCCP dari tiga tipe kelapa sawit Variability of DNA sequence of gene fragment encoding BCCP subunit of ACCase from three types of oil palm Asmini BUDIANI; Djoko SANTOSO; A.R. PURBA PURBA
E-Journal Menara Perkebunan Vol 75, No 1: Juni 2007
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.108 KB) | DOI: 10.22302/iribb.jur.mp.v75i1.149

Abstract

SummaryHeteromeric acetyl-CoA carboxylase (ht-ACCase) is one of key enzymes in palm oilbiosynthesis. Isolation and characterization ofthe gene is an important step in metabolicengineering to increase palm oil content andquality. The objective of this research was toisolate DNA fragment of gene encoding biotincarboxyl carrier protein (BCCP) subunit of ht-ACCase from three different oil palm types(Simalungun, Hibrida and Backcross) andinvestigate the variation of its DNA sequence.Total RNA was isolated from the mesocarp ofoil palm. DNA fragment encoding BCCP wasamplified by means of Reverse TranscriptasePolymerase Chain Reaction (RT-PCR) usingspecific primers with total RNA as a template.The products of RT-PCR were then purifiedfrom the gel, cloned and sequenced. The DNAsequences were analyzed for their homologiesto BCCP gene using BlastN and aligned todetect the sequence variability using ClustalWprogram from BioEdit. The results show thatone of the two RT-PCR products at about 300bp was highly homologous with the geneencoding BCCP from Glycine max, Brassicanapus and Arabidopsis thaliana. Nucleotidesequences of that BCCP fragments from thethree types of oil palm displayed some degreesof variability. Further investigation is neededto analyze the variability of the DNA sequencesof the full-length gene in relation with oilcontent or other characterRingkasanAsetil-CoA karboksilase heteromerik (ht-ACCase) merupakan salah satu enzim kuncidalam biosintesis minyak sawit. Isolasi dankarakterisasi gen tersebut merupakan langkahpenting dalam upaya rekayasa metabolismeuntuk peningkatan rendemen dan kualitasminyak sawit. Penelitian ini bertujuan untukmengisolasi fragmen DNA penyandi subunitbiotin carboxyl carrier protein (BCCP) dari ht-ACCase dari tiga tipe kelapa sawit yang ber-beda (Simalungun, Hibrida dan Backcross)dan mempelajari keragaman susunan nukleo-tidanya. RNA total diisolasi dari mesokarpbuah sawit. Fragmen gen penyandi BCCPdiamplifikasi dengan Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) meng-gunakan primer spesifik dan templat RNA total.Fragmen hasil RT-PCR dimurnikan dari gel,diklon kemudian disekuen. Sekuen DNA yangdiperoleh dianalisis homologinya dengan genBCCP menggunakan BlastN dan disejajarkanuntuk mengetahui keragamannya mengguna-kan program ClustalW dari BioEdit. Hasilnyamenunjukkan bahwa satu dari dua fragmenhasil RT-PCR yang berukuran sekitar 300 pbmemiliki homologi yang tinggi denganfragmen gen penyandi BCCP dari Glycine max,Brassica napus dan Arabidopsis thaliana.Urutan nukleotida fragmen BCCP dari ketigatipe kelapa sawit menunjukkan keragaman.Perlu analisis lebih lanjut mengenai keragamansekuen DNA dari gen lengkapnya dan dikajihubungannya dengan akumulasi minyak ataukarakter lain
Molecular identification and phylogenetic analysis of Chlorella isolates from Indonesia using rbcL gene Fauziatul FITRIYAH; Yora FARAMITHA; Dini Astika SARI; Irma KRESNAWATY; Tri PANJI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 89, No 1 (2021): April, 2021
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.408

Abstract

Identifying the newly isolated species is crucial to establishing a reliable algal database with successful commercial applications for different biotechnological applications. Morphological identification does not give sufficient description, especially for tiny unicellular microalgae. The rbcL gene encodes the large unit of ribulose-1, 5-bisphosphate carboxylase /oxygenase (Rubisco) has been widely known for barcoding in plants and developed for microalgae molecular identification. In this study, we examined the local strains of green microalgae from Indonesia using the rbcL partial gene sequence to identify the strains. Green microalgae isolates originated from Yogyakarta, Serayu, Gondol, Ancol, Cilegon, and Teluk Jakarta were cultured in f/2 media and harvested for DNA extraction. The DNA extracted was proceeded to PCR using 1AB_rbcL primer pair to amplify the sequences of rbcL gene with target band located at 582 bp, followed by the sequencing of the PCR product was conducted. Molecular identification of local green microalgae isolates was successfully carried out using primers 1AB_rbcL with a genetic similarity of 99% toward identified species in the NCBI database. Among six isolates, TJ, G, S, C, and A isolates were identified as C. pyrenoidosa. Only CP isolate from Yogyakarta identified as C. sorokiniana. Nannochloropsis gaditana rbcL sequence was selected as an outgroup. The phylogenetic analysis indicated that the five isolates of Chlorella belong to one clade and clearly distinguished from C. sorokiniana isolate from Yogyakarta.
Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.457 KB) | DOI: 10.22302/iribb.jur.mp.v76i2.84

Abstract

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN  of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen  membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet.  Hasil analisis BlastX dari sekuen klon cDNA menunjukkan  bahwa gen berukuran  ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.
The effects of seaweed fertilizer on the growth and productivity of upland rice, maize and oil palm grown in green house Pengaruh pupuk rumput laut terhadap pertumbuhan dan produktivitas padi gogo, jagung dan kelapa sawit di rumah kaca Djoko SANTOSO; Tetty CHAIDAMSARI; . SYAFARUDDIN; Dedi Soleh EFFENDI
E-Journal Menara Perkebunan Vol 79, No 2: Desember 2011
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.14 KB) | DOI: 10.22302/iribb.jur.mp.v79i2.61

Abstract

AbstrakSebagai negara kepulauan di daerah tropis, Indonesiakaya akan sumberdaya alam untuk swasembada pangan.Berjuta-juta hektar lahan di Indonesia ditanami tanamanperkebunan, tanaman tahunan yang memiliki masa juvenilyang relatif lama, terutama tanaman kelapa sawit dan karet.Sementara itu, upaya untuk meningkatkan produksi panganterkendala oleh terbatasnya lahan subur. Penelitian yangmengeksplorasi bioregulator alami mampu meningkatkanproduktivitas tanaman, menemukan bahwa Sargasum sp.,rumput laut tipe liar yang di sepanjang pantai beberapawilayah Indonesia, menunjukkan kemampuannya meningkat-kan pertumbuhan dan produktivitas tanaman seperti padi,jagung, tomat dan pertumbuhan kelapa sawit tanpapenambahan pupuk kimia. Percobaan pada padi gogovarietas Batutegi yang ditanam di rumah kaca, menunjuk-kan bahwa bioregulator alami tersebut meningkatkanproduktivitasnya 50% lebih tinggi daripada kontrolnya.Percobaan menggunakan jagung var. Arjuna, tanaman yangtelah diperlakukan dengan bioregulator tersebut mem-produksi dua hingga tiga tongkol, sementara pada tanamankontrol hanya satu tongkol. Percobaan pada tanamankelapa sawit di rumahkaca memperlihatkan bahwa bio-regulator tersebut menginduksi pertumbuhan vegetatifnyasecara signifikan, lebih baik daripada kontrol dengan atautanpa pupuk kimia. Intercropping tanaman kelapa sawitTBM dengan tanaman pangan seperti padi gogo ataujagung, diharapkan lebih menguntungkan bagi usahaperkebunan.AbstractBeing a tropical archipelago, Indonesia is rich withnatural resources enabling more production for food.Millions hectares of Indonesian lands is now planted withestate crops, perennial crops with relatively lengthenjuvenile phase mainly oil palm and rubber. Meanwhile,attempts to increase national food production have beenlimited by availability of fertile lands. Our researchexploring natural bioregulator capable of improving cropproductivity, found that Sargasum sp., a wild sea weedgrown mostly along the coast line in Indonesia, indicated itsability to improve the growth and productivity of crops likerice, maize, tomato and oil palm even though with nochemical fertilizers added. The experiment on upland rice oflocal variety Batutegi planted in greenhouse, demonstratedthe natural bioregulator has increased the rice productivityby at least 50% over the control. The experiment usingmaize var. Arjuna, the bioregulator treated plants has madetwo to three corncobs instead of only one corncob on thecontrol plants. The experiment on the oil palm grown in thenursery showed that the bioregulator has significantlyinduced vegetative growth better than the control with orwithout chemical fertilizers. Intercropping the food crops,rice or maize in the juvenile phase of the oil palmplantations, should be beneficial to the productivity of theplantation.
Aktivitas ACCase mesokarp kelapa sawit dan kloning fragmen gen penyandi ACCase subunit biotin karboksilase ACCase activity of oil palm mesocarp and cloning of gene fragment encoding biotin carboxylase subunit of ACCase Asmini BUDIANI; Djoko SANTOSO; Hajrial ASWIDINNOOR; Antonius SUWANTO
E-Journal Menara Perkebunan Vol 74, No 1: Juni 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.725 KB) | DOI: 10.22302/iribb.jur.mp.v74i1.119

Abstract

Summary Genetic engineering to produce high yielding oil palm might be done by over expressing gene encoding key enzyme for oil biosynthesis in the oil palm mesocarp, one of which is ACCase. The objective of this research was to analyze ACCase activity of mesocarp from several developmental stages of fruit and to clone conserved region cDNA of gene encoding biotin carboxylase subunit of ACCase (BC-htACCase) from oil palm mesocarp. Activity of ACCase was analyzed by HPLC. Amplification of cDNA was done by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate heterologous primer on several annealing temperature and MgCl2 concentration. The cDNA fragment of RT-PCR product was cloned, sequenced and analyzed to confirm that the cloned cDNA was conserved region of BC-htACCase. The result showed that ACCase activity increased from the 14 week to the 20 week-old fruit, and then decreased. Using heterologous degenerate primers, cDNA fragments of BC-htACCase conserved region (469 bp) can be specifically amplified at 60 oC annealing temperature with 2 mM MgCl2 concentration.The result of BlastX analysis showed that the sequence of cloned cDNA fragment was highly homologous with the conserved region of BC-htACCase from Glycine max, Arabidopsis thaliana, Nicotiana tabacum,  and Brassica napus with 243, 237, 236, 231 bit score, and E. value 2e-63, 1e-61, 2e-61 and 5e-60, respectively. Ringkasan Rekayasa genetika untuk menghasilkan bibit kelapa sawit berdaya hasil tinggi dapat ditempuh dengan meningkatkan ekspresi gen penyandi enzim kunci biosintesis minyak pada kelapa sawit, salah satunya adalah ACCase. Tujuan penelitian ini adalah menguji aktivitas ACCase mesokarp beberapa tahap perkem-bangan buah sawit dan mengklon fragmen cDNA daerah konservatif gen penyandi ACCase heteromerik subunit biotin karbok-silase (BC-htACCase) dari mesokarp buah sawit. Aktivitas ACCase dianalisis dengan HPLC. Amplifikasi cDNA dilakukan dengan teknik RT-PCR menggunakan primer degene-rate heterologus pada berbagai suhu penempelan dan konsentrasi MgCl2. Fragmen cDNA hasil RT-PCR diklon, disekuen dan dianalisis untuk mengkonfirmasi bahwa cDNA terklon adalah daerah konservatif BC-htACCase. Hasil penelitian menunjukkan bahwa aktivitas ACCase meningkat dari buah berumur 14 minggu hingga buah berumur  20 minggu, kemudian menurun kembali Dengan primer degenerate heterologus, fragmen cDNA daerah konservatif BC-htACCase  (469 pb) dapat diamplifikasi secara spesifik pada suhu penempelan 60 oC dan konsentrasi MgCl2 2 mM. Hasil analisis BlastX dari sekuen DNA fragmen terklon menunjuk-kan bahwa sekuen tersebut mempunyai homologi tinggi antara lain dengan gen penyandi BC-htACCase dari Glycine max, Arabidopsis thaliana, Nicotiana tabacum dan Brassica napus, masing-masing dengan skor 243, 237, 236, 231 bit, dan E. value 2e-63, 1e-61, 2e-61 dan 5e-60.