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All Journal Jurnal Kedokteran Brawijaya MANDALA of Health Jurnal NERS Universa Medicina
Lantip Rujito
Faculty of Medicine and Health Sciences Jenderal Soedirman University Jl Gumbreg no 1, Purwokerto 53146
Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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HUBUNGAN ANTARA INDEKS MASSA TUBUH (IMT) DENGAN TES FUNGSI PARU Rujito, Lantip; Ristianingrum, Ika; Rahmawati, Indah
MANDALA of Health Vol 4, No 2 (2010): Mandala Of Health
Publisher : Jurusan Kedokteran FK Unsoed

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Abstract

Body mass index (BMI) is a tool of measuring the nutritional status of individu. One of the effects ofobesity is the mechanics disturbance led to abnormality on pulmonary function tests. This study was aimed todetermine the relationship between body mass index with pulmonary function tests (PFTs) on medical studentJenderal Soedirman University. Analityc observational study with cross sectional design was used in thisinvestigation. The sampling technique used proportional random sampling with 82 samples. Pulmonaryfunction tests was classified into vital capacity (VC), tidal volume (TV), inspiration reserve volume (IRV),expiratory reserve volume (ERV), inspiration capacity (IC), forced vital capacity (FVC) dan FEV1.Univariate analysis, which was using table of frequency to see chategorichal variables and central tendencymeasurement to see numerical variables, and bivariate analysis, which was using Pearson and Spearmancorrelation to see the relationship between BMI with PFTs; unpaired t tests and Mann Whitney to know thedifferences of PFTs results between men and women, were used in this research. From the results weconcluded that there are significant relationship between between BMI with VC (p = 0,015), IRV (p = 0,026),IC (p = 0,016), FVC (p = 0,004) and FEV1 (p = 0,021) with low power relationship and was no relationshipBMI with TV (p = 0,489) and ERV (p = 0,231). In the unpaired t test and Mann Whitney we found that thereare differences all PFTs in a group of men and women.
GFR and Blood Lead Levels in Gas Station Workers Based on δ-Alad Gene Polymorphisms Rujito, Lantip; Hanief, Muhammad Nur; Gozali, Paulus; Mulyanto, Joko
Jurnal NERS Vol 10, No 1 (2015): Vol. 10 Nomor 1 April 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.002 KB) | DOI: 10.20473/%oj.Ners101%y74-79

Abstract

Introduction: Lead is a well-known toxic agent that makes an organ’s failure. Lead serum itself is infl uenced by δ-ALAD gene polymorphisms (Amino Levulinic Acid Dehydratase). δ-ALAD gene encodes an ALAD enzyme used for heme synthesis. The Characteristic of gene polymorphism may result in Glomerulo Filtration Rate (GFR) value as mark of renal failure. The goal of this study was to fi nd correlations between blood lead levels with GFR in terms of δ ALAD gene polymorphisms. Method: A cross-sectional design was used to perform this research. Thirty-eight gas stations workers in Banyumas were recruited in this study. δ-ALAD gene polymorphisms were characterized using PCR-RFLP method, while lead serum levels were quantifi ed by Atomic Absorption Spectrophotometer (AAS). In addition, Creatinin serum was done with a spectrophotometer and GFR value was formulated by means of the Schwartz method. Result: The studyshowed that the proportion of ALAD genotype for ALAD 1-1, 1-2 and 2-2 were 94.7%, 5.3%, and 0% respectively. The mean of serum levels in homozygous 1-1 was 15.94 ppb and heterozygote 1-2 was 1.15 ppb. GFR of participants ranged from 71.11 mL/min to 185.20 mL/min with a mean of 117.34mL/min. There was no correlation between serum Pb and GFR (p = 0.19). Study also could not determine the correlation between GFR and ALAD gene Polymorphism. Discussion: Study then concluded that there was no correlation between blood lead levels in the GFR on each δ-ALAD genotypes.Keywords: Lead intoxication, GFR, δ-ALAD, gas station workers
Hair root FMRP expression for screening of fragile X full mutation females Rujito, Lantip; Kustiani, Dwi; Severijnen, Lies Anne; Hanzon, Peter; Faradz, Sultana MH; Willemsen, Rob
Universa Medicina Vol 30, No 1 (2011)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2011.v30.11-21

Abstract

The fragile X syndrome is the most common form of inherited mental retardation in humans, caused by an expansion of the cytosine-guanine-guanine (CGG) repeat in the fragile X mental retardation 1 (FMR1) gene located on the X chromosome. Antibody tests have been developed to identify fragile X patients, based on the presence or absence of fragile mental retardation protein (FMRP) in both lymphocytes and hair roots. The objective of this study was to compare correlations of hair root and lymphocyte FMRP expression with cognitive functioning in female rural area probands carrying the full mutation. Thirty females (normal, premutation, or full mutation) were selected from Indonesian fragile X families and were tested for FMRP expression in lymphocytes and hair roots using the FMRP antibody test. Subject genotype was determined by Southern blot analysis, and IQ equivalent by Raven’s Standard Progressive Matrices. Statistical analysis was by Pearson correlation. FMRP expression in blood lymphocytes was relatively higher than that in hair roots, but hair root FMRP expression was strongly correlated with cognitive functioning in female full mutation carriers (r=0.64, p=0.015), whereas no significant correlation between lymphocyte FMRP and cognitive functioning was found (r=0.31, p= 0.281). Around 14% of subjects had a normal and 7% a borderline IQ level, while 79% had mild mental impairment. In conclusion, hair root FMRP expression may be a useful marker for identification of fragile X full mutation females.
GLUTATHIONE S TRANSFERASE AND CATALASE GENE POLYMORPHISMS DID NOT TEND TO INFLUENCE THE SEVERITY OF HEMOGLOBIN E/β-THALASSEMIA Rujito, Lantip; Widodo, Yundandhika Rizki; Sakina, Ghaida; Santosa, Qodri; Hapsari, Ariadne Tiara
Universa Medicina Vol 39, No 1 (2020)
Publisher : Faculty of Medicine, Trisakti University

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Abstract

BackgroundThalassemia, a monogenic genetic disease of red blood cells, is spread widely throughout the world. Glutathione S transferase (GST) enzymes have an antioxidant role in detoxification processes of toxic substances This study aimed to determine the role of the genetic modifier genes GSTT1 and GSTM1, and the catalase (CAT) gene in clinical degrees of hemoglobin (Hb)E/? thalassemia. MethodsSixty HbE/? Thalassemia patients were examined to determine their clinical pictures. Clinical score was based on age when thalassemia symptoms appeared, time of diagnosis, time of first blood transfusion, pre-transfusion hemoglobin concentration, frequency of transfusions, and enlargement of spleen. Ferritin concentration was also obtained from medical records. Gene polymorphisms of GSTT1, GSTM1, and CAT were measured using PCR and PCR-RFLP methods. Clinical scores were categorized into mild (0-3.5), moderate (4-7), and severe (7.5-10) degrees, while ferritin level was expressed in mg/dL. One way Anova was used to analyze the data. ResultsThe clinical appearance showed that severe, moderate, and mild degrees accounted for 42%, 45%, and 13%, respectively. The majority had a high ferritin level of more than 5000 mg/dL (67%). GSTT1 null, GSTM1 null, and CAT minor allele genotypes were 21.7%, 33.3%, and 12.1%, respectively. GSTT1, GSTM1, and CAT genotypes had no impact on the severity of thalassemia patients (p=0.091, p=0.082, and p=0.141, respectively).ConclusionGSTT1, GSTM1, CAT gene polymorphisms tend to be a minor aspect of severity of clinical outcome for HbE/â thalassemia patients and should be not considered a routine laboratory check.
Uji Diagnostik Indeks Darah dan Identifikasi Molekuler Karier Talasemia β pada Pendonor Darah di Banyumas Hapsari, Ariadne Tiara; Rujito, Lantip
Jurnal Kedokteran Brawijaya Vol 28, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (866.366 KB) | DOI: 10.21776/ub.jkb.2015.028.03.13

Abstract

Talasemia menempati kelainan genetik yang paling umum di seluruh dunia dengan prevalensi karier talasemia di Indonesia adalah sekitar 3-10%. Banyumas merupakan salah satu wilayah yang menyumbang angka prevalensi penderita talasemia yang cukup besar. Salah satu perangkat dalam program pencegahan terpadu adalah memastikan diagnosa molekuler pada tahap skrining sesuai dengan mutasi lokal. Tujuan dari penelitian adalah untuk mengetahui nilai uji diagnostik indeks darah dan karakterisitik mutasi talasemia β pada pendonor darah yang dicurigai karier talasemia. Subjek penelitian adalah 183 pendonor darah rutin pada PMI Banyumas. Skrining awal menggunakan indeks darah MCV, MCH, dan Hemoglobin elektroforesis. Karakteristik molekuler dilakukan dengan teknik PCR-RFLP dan teknik ARMS.  Hasil penelitian menunjukkan bahwa MCV memiliki nilai sensitivitas 81,3%, dan spesifisitas 95,8% sedangkan MCH menunjukkan sensitivitas sebesar 80% dengan spesifisitas 97,5%. Mutasi IVS-1 nt 5 (G>C) merupakan mutasi tersering disusul dengan HBE (codon 26) dan IVS1 nt 1(G>T).Kata Kunci: Identifikasi molekuler, indeks darah, karier talasemia
Co-Authors Ariadne Tiara Hapsari Dwi Kustiani, Dwi Ika Ristianingrum, Ika Indah Rahmawati Joko Mulyanto, Joko Lies Anne Severijnen, Lies Anne Muhammad Nur Hanief, Muhammad Nur Paulus Gozali, Paulus Peter Hanzon, Peter Qodri Santosa, Qodri Rob Willemsen, Rob Sakina, Ghaida Sultana MH Faradz Widodo, Yundandhika Rizki